##################################
# NO CUT ENZYMES (  178 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       BalI        BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BbvI        BcgI        BclI        BglI        BglII       
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      Cac8I       CelII       Cfr10I      ClaI        
CpoI        DraI        DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        Fnu4HI      FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        ItaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MspA1I      MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        TseI        Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AfaI          109  GTAC                 GT^AC
AflII         244  CTTAAG               C^TTAAG
AluI           52  AGCT                 AG^CT
AlwNI         179  CAGGTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvrII         601  CCTAGG               C^CTAGG
BanII         336  GGGCTC               G[GA]GC[CT]^C
BfrI          244  CTTAAG               C^TTAAG
BlnI          601  CCTAGG               C^CTAGG
BmyI          336  GGGCTC               G[AGT]GC[ACT]^C
BpmI          629  CTCCAG               CTGGAG(16/14)
BsaWI         683  TCCGGT               [AT]^CCGG[AT]
Bsp1286I      336  GGGCTC               G[AGT]GC[ACT]^C
BsrI          332  ACTGG                ACTGG(1/-1)
BstYI         272  GGATCT               [GA]^GATC[CT]
CfoI          531  GCGC                 GCG^C
DdeI          185  CTGAG                C^T[ACGT]AG
DraIII        197  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI           16  CTCTTC               CTCTTC(1/4)
EcoT14I       601  CCTAGG               C^C[AT][AT]GG
HaeII         530  AGCGCC               [GA]GCGC^[CT]
HhaI          531  GCGC                 GCG^C
HinP1I        531  GCGC                 G^CGC
Ksp632I        16  CTCTTC               CTCTTC(1/4)
MflI          272  GGATCT               [GA]^GATC[CT]
MseI          245  TTAA                 T^TAA
MslI          486  CATCTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PpuMI         180  AGGTCCT              [GA]G^G[AT]CC[CT]
RsaI          109  GTAC                 GT^AC
SexAI         198  ACCTGGT              A^CC[AT]GGT
SmlI          244  CTTAAG               C^T[CT][GA]AG
StyI          601  CCTAGG               C^C[AT][AT]GG
Tru9I         245  TTAA                 T^TAA
TspRI         549  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoII         272  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AlwI          610  GATCC                GGATC(4/5)
              272  GGATC               
BciVI          71  GGATAC               GTATCC(6/5)
              249  GTATCC              
BseRI          65  GAGGAG               GAGGAG(10/8)
              526  GAGGAG              
DraII         142  GGGGCCT              [GA]G^G[ACGT]CC[CT]
              180  AGGTCCT             
EcoO109I      142  GGGGCCT              [GA]G^G[ACGT]CC[CT]
              180  AGGTCCT             
MaeII          75  ACGT                 A^CGT
              675  ACGT                
MboII          17  TCTTC                GAAGA(8/7)
              128  TCTTC               
MwoI           26  GCTTTTTCGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              125  GCATCTTCAGC         
PleI          235  GACTC                GAGTC(4/5)
              187  GAGTC               
SfcI          454  CTACAG               C^T[GA][CT]AG
              569  CTACAG              
Tsp45I        260  GTCAC                ^GT[GC]AC
              493  GTGAC               
XcmI          322  CCAGCACCAGACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              432  CCAGGAGGGTCTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AvaII       BcnI        BfaI        BsaJI       BsiYI       
BslI        BsmAI       BsmFI       BssKI       BstF5I      
BstNI       Cfr13I      DpnII       EcoRII      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
MaeI        MaeIII      MboI        MnlI        MspI        
MvaI        NciI        NdeII       NlaIV       Sau3AI      
Sau96I      ScrFI       SfaNI       TfiI