Restriction map for
pF1KB7326
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Aor51HI ApaI
AscI Asp718I AviII AvrII BbeI
BcgI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PflMI
PinAI PmeI PpuMI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SspBI StuI
SwaI Van91I XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccI 19 GTCTAC GT^[AC][GT]AC
Alw44I 1801 GTGCAC G^TGCAC
AocI 2765 CCTCAGG CC^T[ACGT]AGG
ApaLI 1801 GTGCAC G^TGCAC
AspI 2019 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 125 GGACC G^G[AT]CC
BamHI 993 GGATCC G^GATCC
BanII 1097 GAGCTC G[GA]GC[CT]^C
BbrPI 307 CACGTG CAC^GTG
BbsI 3224 GAAGAC GAAGAC(2/6)
BclI 1215 TGATCA T^GATCA
BcnI 2873 CCGGG CC^[GC]GG
BpuAI 3224 GAAGAC GAAGAC(2/6)
BsaAI 307 CACGTG [CT]AC^GT[GA]
BspHI 2919 TCATGA T^CATGA
BsrBI 1189 CCGCTC CCGCTC(-3/-3)
Bsu36I 2765 CCTCAGG CC^T[ACGT]AGG
Eco81I 2765 CCTCAGG CC^T[ACGT]AGG
FbaI 1215 TGATCA T^GATCA
HapII 2873 CCGG C^CGG
HpaII 2873 CCGG C^CGG
MspI 2873 CCGG C^CGG
NciI 2873 CCGGG CC^[GC]GG
NcoI 1806 CCATGG C^CATGG
PaeR7I 212 CTCGAG C^TCGAG
PflFI 2019 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 307 CACGTG CAC^GTG
PmlI 307 CACGTG CAC^GTG
RcaI 2919 TCATGA T^CATGA
SacI 1097 GAGCTC GAGCT^C
SpeI 3247 ACTAGT A^CTAGT
SphI 3306 GCATGC GCATG^C
Tth111I 2019 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 212 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 35 enzymes) #
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AflIII 1142 ACATGT A^C[GA][CT]GT
2250 ACATGT
AhdI 1030 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1141 GACATGTGGTC
AlwNI 1826 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
2041 CAGATGCTG
AseI 2428 ATTAAT AT^TAAT
3060 ATTAAT
AsnI 2428 ATTAAT AT^TAAT
3060 ATTAAT
Asp700I 787 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1171 GAATTATTTC
AspEI 1030 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1141 GACATGTGGTC
AvaI 212 CTCGAG C^[CT]CG[GA]G
2586 CCCGAG
BalI 624 TGGCCA TGG^CCA
917 TGGCCA
BbvCI 2224 CCTCAGC CCTCAGC(-5/-2)
2263 CCTCAGC
BciVI 2938 GTATCC GTATCC(6/5)
3633 GTATCC
BglI 2052 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3310 GCCCACCTGGC
BsaI 1552 GAGACC GGTCTC(1/5)
2796 GAGACC
BseRI 2763 CTCCTC GAGGAG(10/8)
394 GAGGAG
BsiYI 166 CCCACTAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
319 CCTCAGATTGG
BslI 166 CCCACTAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
319 CCTCAGATTGG
BsmI 787 GAATGC GAATGC(1/-1)
3139 GCATTC
BsoBI 212 CTCGAG C^[CT]CG[GA]G
2586 CCCGAG
BspLU11I 1142 ACATGT A^CATGT
2250 ACATGT
BspMI 3588 ACCTGC ACCTGC(4/8)
265 GCAGGT
DraII 360 AGGCCCC [GA]G^G[ACGT]CC[CT]
1970 AGGCCCC
DsaI 121 CCACGG C^C[GA][CT]GG
1806 CCATGG
Eam1105I 1030 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1141 GACATGTGGTC
EcoO109I 360 AGGCCCC [GA]G^G[ACGT]CC[CT]
1970 AGGCCCC
EcoT14I 1205 CCTTGG C^C[AT][AT]GG
1806 CCATGG
HindIII 2690 AAGCTT A^AGCTT
3233 AAGCTT
MluNI 624 TGGCCA TGG^CCA
917 TGGCCA
MscI 624 TGGCCA TGG^CCA
917 TGGCCA
NdeI 29 CATATG CA^TATG
3028 CATATG
PshBI 2428 ATTAAT AT^TAAT
3060 ATTAAT
SapI 1348 GAAGAGC GCTCTTC(1/4)
2818 GAAGAGC
SspI 979 AATATT AAT^ATT
2710 AATATT
StyI 1205 CCTTGG C^C[AT][AT]GG
1806 CCATGG
XbaI 1590 TCTAGA T^CTAGA
2679 TCTAGA
XmnI 787 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1171 GAATTATTTC
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BanI BbvI BfaI
BmyI BpmI BsaJI BsgI BsiHKAI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EarI EcoRII Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII