Restriction map for
pF1KE0226
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApaI
ApoI AseI AsnI Asp700I Asp718I
AspEI AspI AvaII AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII Hin1I HincII HindII
HindIII HinfI HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MseI MslI MunI NarI
NciI NcoI NdeI NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tru9I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Alw44I 91 GTGCAC G^TGCAC
AlwI 186 GATCC GGATC(4/5)
ApaLI 91 GTGCAC G^TGCAC
AscI 139 GGCGCGCC GG^CGCGCC
AvaI 59 CTCGGG C^[CT]CG[GA]G
BalI 257 TGGCCA TGG^CCA
BanII 277 GAGCTC G[GA]GC[CT]^C
BseRI 274 GAGGAG GAGGAG(10/8)
BsoBI 59 CTCGGG C^[CT]CG[GA]G
BsrDI 203 GCAATG GCAATG(2/0)
BsrFI 136 GCCGGC [GA]^CCGG[CT]
BssHII 140 GCGCGC G^CGCGC
BstAPI 93 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 185 AGATCC [GA]^GATC[CT]
Cfr10I 136 GCCGGC [GA]^CCGG[CT]
DpnII 186 GATC ^GATC
DsaI 80 CCGTGG C^C[GA][CT]GG
EaeI 257 TGGCCA [CT]^GGCC[GA]
EarI 54 CTCTTC CTCTTC(1/4)
EcoT14I 38 CCAAGG C^C[AT][AT]GG
HapII 137 CCGG C^CGG
HgaI 243 GCGTC GACGC(5/10)
HpaII 137 CCGG C^CGG
Ksp632I 54 CTCTTC CTCTTC(1/4)
MaeII 9 ACGT A^CGT
MboI 186 GATC ^GATC
MflI 185 AGATCC [GA]^GATC[CT]
MluNI 257 TGGCCA TGG^CCA
MscI 257 TGGCCA TGG^CCA
MspA1I 179 CCGCTG C[AC]G^C[GT]G
MspI 137 CCGG C^CGG
NaeI 136 GCCGGC GCC^GGC
NdeII 186 GATC ^GATC
NgoMIV 136 GCCGGC G^CCGGC
NlaIV 133 GGAGCC GG[ACGT]^[ACGT]CC
SacI 277 GAGCTC GAGCT^C
SapI 53 GCTCTTC GCTCTTC(1/4)
Sau3AI 186 GATC ^GATC
StyI 38 CCAAGG C^C[AT][AT]GG
Tsp509I 115 AATT ^AATT
TspRI 146 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 185 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AfaI 150 GTAC GT^AC
171 GTAC
AspHI 91 GTGCAC G[AT]GC[AT]^C
277 GAGCTC
BbsI 225 GAAGAC GAAGAC(2/6)
357 GAAGAC
BmyI 91 GTGCAC G[AGT]GC[ACT]^C
277 GAGCTC
BpmI 166 CTGGAG CTGGAG(16/14)
190 CTGGAG
BpuAI 225 GAAGAC GAAGAC(2/6)
357 GAAGAC
BsaJI 38 CCAAGG C^C[ACGT][ACGT]GG
80 CCGTGG
BsiHKAI 91 GTGCAC G[AT]GC[AT]^C
277 GAGCTC
Bsp1286I 91 GTGCAC G[AGT]GC[ACT]^C
277 GAGCTC
BsrBI 51 CCGCTC CCGCTC(-3/-3)
124 GAGCGG
BsrI 255 ACTGG ACTGG(1/-1)
145 CCAGT
BstF5I 237 CATCC GGATG(2/0)
261 CATCC
Cfr13I 84 GGGCC G^G[ACGT]CC
210 GGCCC
FokI 237 CATCC GGATG(9/13)
261 CATCC
HphI 246 TCACC GGTGA(8/7)
302 TCACC
MaeIII 245 GTCAC ^GT[ACGT]AC
301 GTCAC
MnlI 249 CCTC CCTC(7/6)
274 GAGG
RsaI 150 GTAC GT^AC
171 GTAC
Sau96I 84 GGGCC G^G[ACGT]CC
210 GGCCC
Tsp45I 245 GTCAC ^GT[GC]AC
301 GTCAC
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AccII AciI AluI BbvI BsiYI
BslI BssKI BstNI BstUI Cac8I
CfoI DdeI EcoRII FauI Fnu4HI
HaeIII HhaI HinP1I ItaI MboII
MvaI MvnI MwoI ScrFI TseI