Restriction map for
pF1KE0106
##################################
# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MluI MroI
MseI MslI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 347 CGCG CG^CG
AcsI 715 GAATTC [GA]^AATT[CT]
AfaI 825 GTAC GT^AC
ApaI 625 GGGCCC GGGCC^C
ApoI 715 GAATTC [GA]^AATT[CT]
BbsI 450 GAAGAC GAAGAC(2/6)
BbvCI 602 CCTCAGC CCTCAGC(-5/-2)
BcnI 410 CCCGG CC^[GC]GG
BpuAI 450 GAAGAC GAAGAC(2/6)
BseRI 134 CTCCTC GAGGAG(10/8)
BsgI 274 CTGCAC GTGCAG(16/14)
BsiEI 287 CGGCCG CG[GA][CT]^CG
BspMI 293 ACCTGC ACCTGC(4/8)
BsrFI 248 GCCGGC [GA]^CCGG[CT]
BsrI 267 CCAGT ACTGG(1/-1)
BstUI 347 CGCG CG^CG
BstXI 828 CCAAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 548 GGATCT [GA]^GATC[CT]
Cfr10I 248 GCCGGC [GA]^CCGG[CT]
DraIII 772 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 28 CCGTGG C^C[GA][CT]GG
EagI 287 CGGCCG C^GGCCG
EarI 476 CTCTTC CTCTTC(1/4)
EclXI 287 CGGCCG C^GGCCG
Eco52I 287 CGGCCG C^GGCCG
EcoRI 715 GAATTC G^AATTC
EcoT14I 332 CCAAGG C^C[AT][AT]GG
FauI 300 CCCGC CCCGC(4/6)
HaeII 245 AGCGCC [GA]GCGC^[CT]
HphI 102 GGTGA GGTGA(8/7)
Ksp632I 476 CTCTTC CTCTTC(1/4)
MaeIII 103 GTGAC ^GT[ACGT]AC
MflI 548 GGATCT [GA]^GATC[CT]
MvnI 347 CGCG CG^CG
NaeI 248 GCCGGC GCC^GGC
NciI 410 CCCGG CC^[GC]GG
NgoMIV 248 GCCGGC G^CCGGC
RsaI 825 GTAC GT^AC
SapI 475 GCTCTTC GCTCTTC(1/4)
StyI 332 CCAAGG C^C[AT][AT]GG
Tsp45I 103 GTGAC ^GT[GC]AC
TspRI 351 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 548 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 105 GACGCC G[GA]^CG[CT]C
365 GACGCC
AlwI 357 GATCC GGATC(4/5)
548 GGATC
BalI 432 TGGCCA TGG^CCA
837 TGGCCA
BanI 325 GGCACC G^G[CT][GA]CC
532 GGCACC
BanII 227 GAGCCC G[GA]GC[CT]^C
625 GGGCCC
BpmI 137 CTCCAG CTGGAG(16/14)
766 CTGGAG
BsaHI 105 GACGCC G[GA]^CG[CT]C
365 GACGCC
BsaI 33 GAGACC GGTCTC(1/5)
221 GAGACC
BsmFI 66 GGGAC GGGAC(10/14)
611 GGGAC
BstAPI 732 GCAGACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
771 GCACGGAGTGC
CfoI 246 GCGC GCG^C
348 GCGC
DpnII 357 GATC ^GATC
549 GATC
HapII 249 CCGG C^CGG
411 CCGG
HgaI 105 GACGC GACGC(5/10)
365 GACGC
HhaI 246 GCGC GCG^C
348 GCGC
Hin1I 105 GACGCC G[GA]^CG[CT]C
365 GACGCC
HinP1I 246 GCGC G^CGC
348 GCGC
HincII 484 GTCAAC GT[CT]^[GA]AC
523 GTTGAC
HindII 484 GTCAAC GT[CT]^[GA]AC
523 GTTGAC
HpaII 249 CCGG C^CGG
411 CCGG
MboI 357 GATC ^GATC
549 GATC
MluNI 432 TGGCCA TGG^CCA
837 TGGCCA
MscI 432 TGGCCA TGG^CCA
837 TGGCCA
MspI 249 CCGG C^CGG
411 CCGG
NdeII 357 GATC ^GATC
549 GATC
NlaIII 150 CATG CATG^
430 CATG
PstI 57 CTGCAG CTGCA^G
730 CTGCAG
Sau3AI 357 GATC ^GATC
549 GATC
SmlI 313 CTCAAG C^T[CT][GA]AG
748 CTCAAG
TaqI 219 TCGA T^CGA
761 TCGA
TfiI 426 GATTC G^A[AT]TC
468 GATTC
Tsp509I 388 AATT ^AATT
716 AATT
TthHB8I 219 TCGA T^CGA
761 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AlwNI AspHI AvaII
BbvI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DraII
EaeI EcoNI EcoO109I EcoRII Fnu4HI
FokI HaeIII HinfI ItaI MboII
MnlI MspA1I MvaI MwoI NlaIV
PleI PpuMI PvuII Sau96I ScrFI
SfaNI SfcI TseI