Restriction map for
pF1KE0217
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BciVI BclI BcnI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KpnI Ksp632I
KspI MaeIII MflI MluI MroI
MslI MspA1I MspI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI PstI PvuI PvuII
RcaI RsrII SacII SalI SapI
Sau96I SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tsp45I Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 178 GTAGAC GT^[AC][GT]AC
AclI 456 AACGTT AA^CGTT
AfaI 93 GTAC GT^AC
AflIII 89 ACGTGT A^C[GA][CT]GT
AlwI 7 GGATC GGATC(4/5)
BalI 48 TGGCCA TGG^CCA
BanII 69 GAGCTC G[GA]GC[CT]^C
BbsI 229 GAAGAC GAAGAC(2/6)
BbvI 282 GCAGC GCAGC(8/12)
BcgI 18 CGACAGCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 40 CTAG C^TAG
BglI 241 GCCGATGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 229 GAAGAC GAAGAC(2/6)
BsaBI 168 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 29 CCGTGG C^C[ACGT][ACGT]GG
BsiYI 115 CCATTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 115 CCATTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 221 GGGAC GGGAC(10/14)
BsmI 449 GCATTC GAATGC(1/-1)
BsrI 76 CCAGT ACTGG(1/-1)
BssKI 148 CCAGG ^CC[ACGT]GG
BstF5I 417 GGATG GGATG(2/0)
BstNI 148 CCAGG CC^[AT]GG
DraI 54 TTTAAA TTT^AAA
DsaI 29 CCGTGG C^C[GA][CT]GG
EcoRII 148 CCAGG ^CC[AT]GG
FauI 4 GCGGG CCCGC(4/6)
Fnu4HI 282 GCAGC GC^[ACGT]GC
FokI 417 GGATG GGATG(9/13)
HindIII 508 AAGCTT A^AGCTT
HinfI 223 GACTC G^A[ACGT]TC
ItaI 282 GCAGC GC^[ACGT]GC
MaeI 40 CTAG C^TAG
MamI 168 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 467 CAATTG C^AATTG
MluNI 48 TGGCCA TGG^CCA
MscI 48 TGGCCA TGG^CCA
MunI 467 CAATTG C^AATTG
MvaI 148 CCAGG CC^[AT]GG
MwoI 241 GCCGATGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 141 CATG CATG^
PflMI 115 CCATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 223 GACTC GAGTC(4/5)
Psp1406I 456 AACGTT AA^CGTT
RsaI 93 GTAC GT^AC
SacI 69 GAGCTC GAGCT^C
ScrFI 148 CCAGG CC^[ACGT]GG
TaqI 254 TCGA T^CGA
TseI 282 GCAGC G^C[AT]GC
TspRI 11 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 254 TCGA T^CGA
Van91I 115 CCATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AciI 4 GCGG CCGC(-3/-1)
85 GCGG
AcsI 294 AAATTC [GA]^AATT[CT]
380 AAATTC
ApoI 294 AAATTC [GA]^AATT[CT]
380 AAATTC
AspHI 69 GAGCTC G[AT]GC[AT]^C
391 GAGCAC
BpmI 74 CTCCAG CTGGAG(16/14)
496 CTCCAG
BsaI 144 GAGACC GGTCTC(1/5)
491 GAGACC
BsiHKAI 69 GAGCTC G[AT]GC[AT]^C
391 GAGCAC
BsmAI 144 GAGAC GTCTC(1/5)
491 GAGAC
DdeI 225 CTCAG C^T[ACGT]AG
289 CTCAG
EaeI 14 TGGCCG [CT]^GGCC[GA]
48 TGGCCA
HaeIII 15 GGCC GG^CC
49 GGCC
MboII 229 GAAGA GAAGA(8/7)
322 GAAGA
MseI 55 TTAA T^TAA
131 TTAA
SfaNI 25 GATGC GCATC(5/9)
173 GCATC
Tru9I 55 TTAA T^TAA
131 TTAA
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
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AluI BmyI Bsp1286I DpnII HphI
MaeII MboI MnlI NdeII Sau3AI
Tsp509I