Restriction map for
pF1KB6308
##################################
# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BbvI BcgI BciVI
BclI BcnI BfrI BglI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII ClaI CpoI DdeI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
TseI TspRI Tth111I Van91I XbaI
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AflIII 806 ACGTGT A^C[GA][CT]GT
AgeI 444 ACCGGT A^CCGGT
Alw44I 688 GTGCAC G^TGCAC
AlwI 457 GATCC GGATC(4/5)
AlwNI 455 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 688 GTGCAC G^TGCAC
AspHI 688 GTGCAC G[AT]GC[AT]^C
AvaI 266 CTCGAG C^[CT]CG[GA]G
AvaII 450 GGTCC G^G[AT]CC
AvrII 415 CCTAGG C^CTAGG
BglII 618 AGATCT A^GATCT
BlnI 415 CCTAGG C^CTAGG
BmyI 688 GTGCAC G[AGT]GC[ACT]^C
BsaI 342 GGTCTC GGTCTC(1/5)
BsaWI 444 ACCGGT [AT]^CCGG[AT]
BseRI 149 GAGGAG GAGGAG(10/8)
BsgI 87 CTGCAC GTGCAG(16/14)
BsiHKAI 688 GTGCAC G[AT]GC[AT]^C
BsiYI 399 CCCCATAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 399 CCCCATAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 288 GAATGC GAATGC(1/-1)
BsoBI 266 CTCGAG C^[CT]CG[GA]G
Bsp1286I 688 GTGCAC G[AGT]GC[ACT]^C
BspHI 798 TCATGA T^CATGA
BspMI 85 ACCTGC ACCTGC(4/8)
BsrFI 444 ACCGGT [GA]^CCGG[CT]
BsrI 481 CCAGT ACTGG(1/-1)
BssKI 293 CCAGG ^CC[ACGT]GG
BstNI 293 CCAGG CC^[AT]GG
BstXI 14 CCAAGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 832 GCGC GCG^C
Cfr10I 444 ACCGGT [GA]^CCGG[CT]
DraI 613 TTTAAA TTT^AAA
DraII 28 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 347 CCGTGG C^C[GA][CT]GG
EarI 498 GAAGAG CTCTTC(1/4)
EcoO109I 28 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRII 293 CCAGG ^CC[AT]GG
EcoT14I 415 CCTAGG C^C[AT][AT]GG
Fnu4HI 377 GCCGC GC^[ACGT]GC
HapII 445 CCGG C^CGG
HhaI 832 GCGC GCG^C
HinP1I 832 GCGC G^CGC
HpaII 445 CCGG C^CGG
ItaI 377 GCCGC GC^[ACGT]GC
Ksp632I 498 GAAGAG CTCTTC(1/4)
MslI 402 CATAAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 445 CCGG C^CGG
MvaI 293 CCAGG CC^[AT]GG
NspI 158 GCATGT [GA]CATG^[CT]
PaeR7I 266 CTCGAG C^TCGAG
PinAI 444 ACCGGT A^CCGGT
PstI 218 CTGCAG CTGCA^G
RcaI 798 TCATGA T^CATGA
ScrFI 293 CCAGG CC^[ACGT]GG
SfaNI 98 GCATC GCATC(5/9)
SmlI 266 CTCGAG C^T[CT][GA]AG
SspI 760 AATATT AAT^ATT
StuI 749 AGGCCT AGG^CCT
StyI 415 CCTAGG C^C[AT][AT]GG
TaqI 267 TCGA T^CGA
TthHB8I 267 TCGA T^CGA
XhoI 266 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 96 CCGC CCGC(-3/-1)
378 CCGC
AcsI 196 GAATTT [GA]^AATT[CT]
570 GAATTT
ApoI 196 GAATTT [GA]^AATT[CT]
570 GAATTT
Asp700I 39 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
183 GAACTTCTTC
BfaI 416 CTAG C^TAG
556 CTAG
BsmAI 214 GTCTC GTCTC(1/5)
343 GTCTC
BsmFI 451 GTCCC GGGAC(10/14)
810 GTCCC
BstYI 456 AGATCC [GA]^GATC[CT]
618 AGATCT
Cac8I 75 GCTTGC GC[ACGT]^[ACGT]GC
138 GCTGGC
HindIII 73 AAGCTT A^AGCTT
112 AAGCTT
HinfI 53 GAATC G^A[ACGT]TC
411 GATTC
HphI 408 GGTGA GGTGA(8/7)
534 TCACC
MaeI 416 CTAG C^TAG
556 CTAG
MaeII 668 ACGT A^CGT
806 ACGT
MflI 456 AGATCC [GA]^GATC[CT]
618 AGATCT
MseI 116 TTAA T^TAA
614 TTAA
NlaIV 28 GGGGCC GG[ACGT]^[ACGT]CC
450 GGTCCC
SfcI 218 CTGCAG C^T[GA][CT]AG
712 CTACAG
TfiI 53 GAATC G^A[AT]TC
411 GATTC
Tru9I 116 TTAA T^TAA
614 TTAA
Tsp45I 131 GTGAC ^GT[GC]AC
385 GTGAC
XhoII 456 AGATCC [GA]^GATC[CT]
618 AGATCT
XmnI 39 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
183 GAACTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AluI BbsI BpuAI BsaJI BstF5I
Cfr13I DpnII FokI HaeIII MaeIII
MboI MboII MnlI MwoI NdeII
NlaIII Sau3AI Sau96I Tsp509I