Restriction map for
pF1KB5952
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# NO CUT ENZYMES ( 104 enzymes) #
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AccIII AclI AcsI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII BbeI
BbrPI BciVI BclI BfrI BglII
BsaAI BsaBI BsaI BsaWI BseAI
BsiWI BspDI BspEI BspLU11I BspMI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eam1105I EarI Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KasI Ksp632I MamI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TfiI Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 198 GACGTC GACGT^C
Acc65I 12 GGTACC G^GTACC
AccI 760 GTCTAC GT^[AC][GT]AC
AcyI 198 GACGTC G[GA]^CG[CT]C
AflIII 1254 ACACGT A^C[GA][CT]GT
Asp718I 12 GGTACC G^GTACC
BcgI 1210 CGAGCCACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 480 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 480 GCTGAGC GC^T[ACGT]AGC
BsaHI 198 GACGTC G[GA]^CG[CT]C
BsgI 720 CTGCAC GTGCAG(16/14)
BsiEI 1049 CGGCCG CG[GA][CT]^CG
BsmI 789 GAATGC GAATGC(1/-1)
Bsp1407I 338 TGTACA T^GTACA
BspHI 800 TCATGA T^CATGA
BsrBI 1545 GAGCGG CCGCTC(-3/-3)
BsrGI 338 TGTACA T^GTACA
CelII 480 GCTGAGC GC^T[ACGT]AGC
EagI 1049 CGGCCG C^GGCCG
EclXI 1049 CGGCCG C^GGCCG
Eco52I 1049 CGGCCG C^GGCCG
EcoNI 204 CCTATCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 768 GGCGCT [GA]GCGC^[CT]
Hin1I 198 GACGTC G[GA]^CG[CT]C
HincII 1354 GTCAAC GT[CT]^[GA]AC
HindII 1354 GTCAAC GT[CT]^[GA]AC
KpnI 12 GGTACC GGTAC^C
KspI 1052 CCGCGG CCGC^GG
MseI 1516 TTAA T^TAA
NaeI 485 GCCGGC GCC^GGC
NcoI 8 CCATGG C^CATGG
NgoMIV 485 GCCGGC G^CCGGC
PflMI 594 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1177 CAGCTG CAG^CTG
RcaI 800 TCATGA T^CATGA
SacI 1446 GAGCTC GAGCT^C
SacII 1052 CCGCGG CCGC^GG
ScaI 160 AGTACT AGT^ACT
SfaNI 783 GATGC GCATC(5/9)
SspBI 338 TGTACA T^GTACA
Tru9I 1516 TTAA T^TAA
Tsp509I 1203 AATT ^AATT
Van91I 594 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AvrII 891 CCTAGG C^CTAGG
942 CCTAGG
BamHI 278 GGATCC G^GATCC
1340 GGATCC
BbsI 1465 GTCTTC GAAGAC(2/6)
1525 GTCTTC
BbvCI 620 CCTCAGC CCTCAGC(-5/-2)
960 CCTCAGC
BlnI 891 CCTAGG C^CTAGG
942 CCTAGG
BpmI 1317 CTCCAG CTGGAG(16/14)
134 CTGGAG
BpuAI 1465 GTCTTC GAAGAC(2/6)
1525 GTCTTC
BsrDI 299 GCAATG GCAATG(2/0)
1612 GCAATG
BssHII 844 GCGCGC G^CGCGC
846 GCGCGC
BssSI 1400 CACGAG CACGAG(-5/-1)
673 CTCGTG
BstF5I 17 CATCC GGATG(2/0)
426 GGATG
BstXI 224 CCAGTGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
524 CCACTGTGCTGG
BstYI 278 GGATCC [GA]^GATC[CT]
1340 GGATCC
FokI 17 CATCC GGATG(9/13)
426 GGATG
MflI 278 GGATCC [GA]^GATC[CT]
1340 GGATCC
NspI 584 ACATGC [GA]CATG^[CT]
1216 ACATGC
PaeR7I 1019 CTCGAG C^TCGAG
1135 CTCGAG
PpuMI 139 GGGTCCC [GA]G^G[AT]CC[CT]
945 AGGACCC
SmlI 1019 CTCGAG C^T[CT][GA]AG
1135 CTCGAG
TaqI 1020 TCGA T^CGA
1136 TCGA
TthHB8I 1020 TCGA T^CGA
1136 TCGA
XhoI 1019 CTCGAG C^TCGAG
1135 CTCGAG
XhoII 278 GGATCC [GA]^GATC[CT]
1340 GGATCC
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BglI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfcI
SmaI StyI TseI Tsp45I TspRI
XcmI XmaCI XmaI