################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AhdI Alw44I AlwNI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbeI BbvCI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaBI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BsrDI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI XbaI XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AcsI 841 AAATTT [GA]^AATT[CT] AgeI 1502 ACCGGT A^CCGGT AocI 573 CCTGAGG CC^T[ACGT]AGG Aor51HI 662 AGCGCT AGC^GCT ApaI 382 GGGCCC GGGCC^C ApoI 841 AAATTT [GA]^AATT[CT] Asp700I 510 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 148 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 925 CACGTG CAC^GTG BbsI 751 GAAGAC GAAGAC(2/6) BfaI 799 CTAG C^TAG BglI 692 GCCTTCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 778 AGATCT A^GATCT BpuAI 751 GAAGAC GAAGAC(2/6) BsaAI 925 CACGTG [CT]AC^GT[GA] BsaI 1228 GGTCTC GGTCTC(1/5) BsaWI 1502 ACCGGT [AT]^CCGG[AT] BsrBI 1057 CCGCTC CCGCTC(-3/-3) BsrFI 1502 ACCGGT [GA]^CCGG[CT] BssSI 784 CTCGTG CACGAG(-5/-1) BstXI 1433 CCATCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 778 AGATCT [GA]^GATC[CT] Bsu36I 573 CCTGAGG CC^T[ACGT]AGG Cfr10I 1502 ACCGGT [GA]^CCGG[CT] Eco47III 662 AGCGCT AGC^GCT Eco81I 573 CCTGAGG CC^T[ACGT]AGG EcoRV 358 GATATC GAT^ATC HphI 461 TCACC GGTGA(8/7) MaeI 799 CTAG C^TAG MflI 778 AGATCT [GA]^GATC[CT] PflFI 148 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 924 CCACGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1502 ACCGGT A^CCGGT PmaCI 925 CACGTG CAC^GTG PmlI 925 CACGTG CAC^GTG PpuMI 104 AGGACCT [GA]G^G[AT]CC[CT] PvuII 1045 CAGCTG CAG^CTG SacI 825 GAGCTC GAGCT^C SapI 1407 GCTCTTC GCTCTTC(1/4) SmaI 1002 CCCGGG CCC^GGG SmlI 1282 CTCAAG C^T[CT][GA]AG TaqI 330 TCGA T^CGA Tsp45I 146 GTGAC ^GT[GC]AC Tsp509I 842 AATT ^AATT Tth111I 148 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 330 TCGA T^CGA Van91I 924 CCACGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 778 AGATCT [GA]^GATC[CT] XmaCI 1002 CCCGGG C^CCGGG XmaI 1002 CCCGGG C^CCGGG XmnI 510 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AflIII 23 ACATGT A^C[GA][CT]GT 1478 ACATGT BalI 158 TGGCCA TGG^CCA 567 TGGCCA BanI 1069 GGCACC G^G[CT][GA]CC 1143 GGTGCC BpmI 68 CTCCAG CTGGAG(16/14) 419 CTGGAG BseRI 1213 GAGGAG GAGGAG(10/8) 1288 GAGGAG BsgI 1048 CTGCAC GTGCAG(16/14) 991 GTGCAG BsmAI 178 GAGAC GTCTC(1/5) 1229 GTCTC Bsp1407I 1382 TGTACA T^GTACA 1427 TGTACA BspLU11I 23 ACATGT A^CATGT 1478 ACATGT BsrGI 1382 TGTACA T^GTACA 1427 TGTACA BssHII 1264 GCGCGC G^CGCGC 1314 GCGCGC BstF5I 1434 CATCC GGATG(2/0) 3 GGATG EarI 1408 CTCTTC CTCTTC(1/4) 193 GAAGAG EcoT14I 294 CCAAGG C^C[AT][AT]GG 1130 CCAAGG FokI 1434 CATCC GGATG(9/13) 3 GGATG Ksp632I 1408 CTCTTC CTCTTC(1/4) 193 GAAGAG MaeIII 146 GTGAC ^GT[ACGT]AC 806 GTAAC MluNI 158 TGGCCA TGG^CCA 567 TGGCCA MscI 158 TGGCCA TGG^CCA 567 TGGCCA MseI 170 TTAA T^TAA 719 TTAA MslI 601 CACAAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1434 CATCCACATG NspI 23 ACATGT [GA]CATG^[CT] 1478 ACATGT SspBI 1382 TGTACA T^GTACA 1427 TGTACA StyI 294 CCAAGG C^C[AT][AT]GG 1130 CCAAGG Tru9I 170 TTAA T^TAA 719 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AspHI AvaI AvaII BanII BbvI BcnI BmyI BsaHI BsaJI BsiHKAI BsiYI BslI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI TseI TspRI XcmI