##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI Alw44I AlwNI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BbeI BbvCI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaBI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BsrDI Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoNI EcoO65I EcoRI EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PmeI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TfiI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AcsI 841 AAATTT [GA]^AATT[CT]
AgeI 1502 ACCGGT A^CCGGT
AocI 573 CCTGAGG CC^T[ACGT]AGG
Aor51HI 662 AGCGCT AGC^GCT
ApaI 382 GGGCCC GGGCC^C
ApoI 841 AAATTT [GA]^AATT[CT]
Asp700I 510 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 148 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 925 CACGTG CAC^GTG
BbsI 751 GAAGAC GAAGAC(2/6)
BfaI 799 CTAG C^TAG
BglI 692 GCCTTCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 778 AGATCT A^GATCT
BpuAI 751 GAAGAC GAAGAC(2/6)
BsaAI 925 CACGTG [CT]AC^GT[GA]
BsaI 1228 GGTCTC GGTCTC(1/5)
BsaWI 1502 ACCGGT [AT]^CCGG[AT]
BsrBI 1057 CCGCTC CCGCTC(-3/-3)
BsrFI 1502 ACCGGT [GA]^CCGG[CT]
BssSI 784 CTCGTG CACGAG(-5/-1)
BstXI 1433 CCATCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 778 AGATCT [GA]^GATC[CT]
Bsu36I 573 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1502 ACCGGT [GA]^CCGG[CT]
Eco47III 662 AGCGCT AGC^GCT
Eco81I 573 CCTGAGG CC^T[ACGT]AGG
EcoRV 358 GATATC GAT^ATC
HphI 461 TCACC GGTGA(8/7)
MaeI 799 CTAG C^TAG
MflI 778 AGATCT [GA]^GATC[CT]
PflFI 148 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 924 CCACGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1502 ACCGGT A^CCGGT
PmaCI 925 CACGTG CAC^GTG
PmlI 925 CACGTG CAC^GTG
PpuMI 104 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 1045 CAGCTG CAG^CTG
SacI 825 GAGCTC GAGCT^C
SapI 1407 GCTCTTC GCTCTTC(1/4)
SmaI 1002 CCCGGG CCC^GGG
SmlI 1282 CTCAAG C^T[CT][GA]AG
TaqI 330 TCGA T^CGA
Tsp45I 146 GTGAC ^GT[GC]AC
Tsp509I 842 AATT ^AATT
Tth111I 148 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 330 TCGA T^CGA
Van91I 924 CCACGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 778 AGATCT [GA]^GATC[CT]
XmaCI 1002 CCCGGG C^CCGGG
XmaI 1002 CCCGGG C^CCGGG
XmnI 510 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 23 ACATGT A^C[GA][CT]GT
1478 ACATGT
BalI 158 TGGCCA TGG^CCA
567 TGGCCA
BanI 1069 GGCACC G^G[CT][GA]CC
1143 GGTGCC
BpmI 68 CTCCAG CTGGAG(16/14)
419 CTGGAG
BseRI 1213 GAGGAG GAGGAG(10/8)
1288 GAGGAG
BsgI 1048 CTGCAC GTGCAG(16/14)
991 GTGCAG
BsmAI 178 GAGAC GTCTC(1/5)
1229 GTCTC
Bsp1407I 1382 TGTACA T^GTACA
1427 TGTACA
BspLU11I 23 ACATGT A^CATGT
1478 ACATGT
BsrGI 1382 TGTACA T^GTACA
1427 TGTACA
BssHII 1264 GCGCGC G^CGCGC
1314 GCGCGC
BstF5I 1434 CATCC GGATG(2/0)
3 GGATG
EarI 1408 CTCTTC CTCTTC(1/4)
193 GAAGAG
EcoT14I 294 CCAAGG C^C[AT][AT]GG
1130 CCAAGG
FokI 1434 CATCC GGATG(9/13)
3 GGATG
Ksp632I 1408 CTCTTC CTCTTC(1/4)
193 GAAGAG
MaeIII 146 GTGAC ^GT[ACGT]AC
806 GTAAC
MluNI 158 TGGCCA TGG^CCA
567 TGGCCA
MscI 158 TGGCCA TGG^CCA
567 TGGCCA
MseI 170 TTAA T^TAA
719 TTAA
MslI 601 CACAAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1434 CATCCACATG
NspI 23 ACATGT [GA]CATG^[CT]
1478 ACATGT
SspBI 1382 TGTACA T^GTACA
1427 TGTACA
StyI 294 CCAAGG C^C[AT][AT]GG
1130 CCAAGG
Tru9I 170 TTAA T^TAA
719 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BanII
BbvI BcnI BmyI BsaHI BsaJI
BsiHKAI BsiYI BslI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII ItaI
MaeII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI TseI
TspRI XcmI