Restriction map for
pF1KA1023
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# NO CUT ENZYMES ( 106 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AflIII AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BseAI BsiWI BsmI BspDI BspEI
BspHI BspLU11I BspMI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
ClaI DraI DraIII Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
MaeI MaeIII MfeI MluI MluNI
MroI MscI MslI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PmeI PshBI Psp1406I PvuI
RcaI SalI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SspI SwaI TaqI Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 1822 GACGTC GACGT^C
AccI 220 GTCTAC GT^[AC][GT]AC
AfaI 1401 GTAC GT^AC
AgeI 1675 ACCGGT A^CCGGT
AocI 1784 CCTCAGG CC^T[ACGT]AGG
AvaI 1655 CCCGGG C^[CT]CG[GA]G
BbrPI 277 CACGTG CAC^GTG
BbvCI 822 GCTGAGG CCTCAGC(-5/-2)
BlpI 759 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 759 GCTGAGC GC^T[ACGT]AGC
BsaAI 277 CACGTG [CT]AC^GT[GA]
BsaBI 546 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 998 GAGACC GGTCTC(1/5)
BsiEI 12 CGGCCG CG[GA][CT]^CG
BsoBI 1655 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1400 TGTACA T^GTACA
BsrGI 1400 TGTACA T^GTACA
Bsu36I 1784 CCTCAGG CC^T[ACGT]AGG
CelII 759 GCTGAGC GC^T[ACGT]AGC
CpoI 1015 CGGACCG CG^G[AT]CCG
DrdI 1843 GACGATATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 12 CGGCCG C^GGCCG
EclXI 12 CGGCCG C^GGCCG
Eco52I 12 CGGCCG C^GGCCG
Eco81I 1784 CCTCAGG CC^T[ACGT]AGG
HincII 1825 GTCAAC GT[CT]^[GA]AC
HindII 1825 GTCAAC GT[CT]^[GA]AC
MamI 546 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 557 CCATGG C^CATGG
NspI 105 GCATGC [GA]CATG^[CT]
PinAI 1675 ACCGGT A^CCGGT
PmaCI 277 CACGTG CAC^GTG
PmlI 277 CACGTG CAC^GTG
PpuMI 743 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 1033 CAGCTG CAG^CTG
RsaI 1401 GTAC GT^AC
RsrII 1015 CGGACCG CG^G[AT]CCG
SmaI 1655 CCCGGG CCC^GGG
SphI 105 GCATGC GCATG^C
SspBI 1400 TGTACA T^GTACA
Tsp509I 1190 AATT ^AATT
XmaCI 1655 CCCGGG C^CCGGG
XmaI 1655 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BamHI 372 GGATCC G^GATCC
458 GGATCC
BbeI 320 GGCGCC GGCGC^C
652 GGCGCC
BbsI 525 GAAGAC GAAGAC(2/6)
645 GAAGAC
BpuAI 525 GAAGAC GAAGAC(2/6)
645 GAAGAC
BsaWI 619 ACCGGA [AT]^CCGG[AT]
1675 ACCGGT
BsrDI 272 CATTGC GCAATG(2/0)
1854 CATTGC
BsrFI 350 ACCGGC [GA]^CCGG[CT]
1675 ACCGGT
BssSI 950 CACGAG CACGAG(-5/-1)
973 CACGAG
Cfr10I 350 ACCGGC [GA]^CCGG[CT]
1675 ACCGGT
DraII 77 AGGCCCT [GA]G^G[ACGT]CC[CT]
743 AGGACCT
EcoO109I 77 AGGCCCT [GA]G^G[ACGT]CC[CT]
743 AGGACCT
EcoT14I 557 CCATGG C^C[AT][AT]GG
1799 CCTTGG
KasI 320 GGCGCC G^GCGCC
652 GGCGCC
MseI 262 TTAA T^TAA
440 TTAA
NarI 320 GGCGCC GG^CGCC
652 GGCGCC
PshAI 114 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
213 GACTCCTGTC
SbfI 1068 CCTGCAGG CCTGCA^GG
1380 CCTGCAGG
SfoI 320 GGCGCC GGC^GCC
652 GGCGCC
Sse8387I 1068 CCTGCAGG CCTGCA^GG
1380 CCTGCAGG
StuI 1533 AGGCCT AGG^CCT
1796 AGGCCT
StyI 557 CCATGG C^C[AT][AT]GG
1799 CCTTGG
Tru9I 262 TTAA T^TAA
440 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AspHI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BsrBI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoNI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
SacI SacII SapI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI TseI
TspRI XhoII