Restriction map for
pF1KB7689
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BalI BamHI BbeI BbrPI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BseAI
BsgI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoT14I
FbaI FseI HaeII HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MslI MspA1I MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI StuI StyI SwaI
Tth111I XbaI XcmI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 322 GTCTAC GT^[AC][GT]AC
AccII 1033 CGCG CG^CG
AcyI 831 GGCGTC G[GA]^CG[CT]C
AlwNI 906 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 652 GAGCTC G[AT]GC[AT]^C
AviII 17 TGCGCA TGC^GCA
BanI 894 GGTGCC G^G[CT][GA]CC
BbvCI 786 CCTCAGC CCTCAGC(-5/-2)
BfaI 125 CTAG C^TAG
BglI 747 GCCACACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 668 AGATCT A^GATCT
BsaHI 831 GGCGTC G[GA]^CG[CT]C
BsaI 985 GAGACC GGTCTC(1/5)
BsiHKAI 652 GAGCTC G[AT]GC[AT]^C
BsmAI 985 GAGAC GTCTC(1/5)
BsmI 329 GAATGC GAATGC(1/-1)
BspMI 815 ACCTGC ACCTGC(4/8)
BstUI 1033 CGCG CG^CG
DrdI 859 GACCTCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 28 CCGTGG C^C[GA][CT]GG
EcoRI 255 GAATTC G^AATTC
EcoRV 1018 GATATC GAT^ATC
EcoT22I 115 ATGCAT ATGCA^T
FauI 1031 CCCGC CCCGC(4/6)
FspI 17 TGCGCA TGC^GCA
Hin1I 831 GGCGTC G[GA]^CG[CT]C
MaeI 125 CTAG C^TAG
MvnI 1033 CGCG CG^CG
NsiI 115 ATGCAT ATGCA^T
PaeR7I 803 CTCGAG C^TCGAG
PflMI 221 CCAAAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 664 CTGCAG CTGCA^G
SacI 652 GAGCTC GAGCT^C
SfcI 664 CTGCAG C^T[GA][CT]AG
SspI 766 AATATT AAT^ATT
TaqI 804 TCGA T^CGA
TfiI 564 GAATC G^A[AT]TC
TthHB8I 804 TCGA T^CGA
Van91I 221 CCAAAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 803 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 237 GAATTT [GA]^AATT[CT]
255 GAATTC
AfaI 512 GTAC GT^AC
783 GTAC
ApoI 237 GAATTT [GA]^AATT[CT]
255 GAATTC
AvaII 477 GGTCC G^G[AT]CC
858 GGACC
BanII 67 GGGCTC G[GA]GC[CT]^C
652 GAGCTC
BbsI 388 GAAGAC GAAGAC(2/6)
681 GTCTTC
BpmI 862 CTCCAG CTGGAG(16/14)
34 CTGGAG
BpuAI 388 GAAGAC GAAGAC(2/6)
681 GTCTTC
BsaWI 855 ACCGGA [AT]^CCGG[AT]
891 TCCGGT
BseRI 37 GAGGAG GAGGAG(10/8)
880 GAGGAG
BsmFI 799 GGGAC GGGAC(10/14)
540 GTCCC
BsrFI 3 GCCGGT [GA]^CCGG[CT]
636 GCCGGT
BstEII 612 GGTGACC G^GT[ACGT]ACC
867 GGTCACC
BstPI 612 GGTGACC G^GT[ACGT]ACC
867 GGTCACC
BstYI 104 AGATCC [GA]^GATC[CT]
668 AGATCT
Cfr10I 3 GCCGGT [GA]^CCGG[CT]
636 GCCGGT
EaeI 745 CGGCCA [CT]^GGCC[GA]
973 CGGCCA
EarI 727 GAAGAG CTCTTC(1/4)
759 GAAGAG
EcoO65I 612 GGTGACC G^GT[ACGT]ACC
867 GGTCACC
Ksp632I 727 GAAGAG CTCTTC(1/4)
759 GAAGAG
MflI 104 AGATCC [GA]^GATC[CT]
668 AGATCT
MseI 80 TTAA T^TAA
398 TTAA
NspI 20 GCATGC [GA]CATG^[CT]
117 GCATGC
PleI 801 GACTC GAGTC(4/5)
424 GAGTC
RsaI 512 GTAC GT^AC
783 GTAC
SmaI 63 CCCGGG CCC^GGG
989 CCCGGG
SmlI 70 CTCAAG C^T[CT][GA]AG
803 CTCGAG
SphI 20 GCATGC GCATG^C
117 GCATGC
Tru9I 80 TTAA T^TAA
398 TTAA
Tsp509I 238 AATT ^AATT
256 AATT
TspRI 618 CACTG CA[GC]TG[ACGT][ACGT]^
751 CACTG
XhoII 104 AGATCC [GA]^GATC[CT]
668 AGATCT
XmaCI 63 CCCGGG C^CCGGG
989 CCCGGG
XmaI 63 CCCGGG C^CCGGG
989 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AlwI AvaI BbvI
BcnI BmyI BsaJI BsiYI BslI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TseI Tsp45I