Restriction map for
pF1KB9879
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BclI BglII BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspEI BspHI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I Bsu36I CelII DraI DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
MaeIII MamI MfeI MflI MluI
MroI MslI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI SacI
SalI SbfI ScaI SexAI SfiI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tsp45I
Tsp509I XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 48 enzymes) #
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AflII 214 CTTAAG C^TTAAG
Asp700I 816 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 178 GTGCTC G[AT]GC[AT]^C
AspI 1006 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 407 TGGCCA TGG^CCA
BbrPI 175 CACGTG CAC^GTG
BbsI 1531 GAAGAC GAAGAC(2/6)
BbvCI 439 GCTGAGG CCTCAGC(-5/-2)
BcgI 777 CGATGCTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 799 GGATAC GTATCC(6/5)
BfaI 1528 CTAG C^TAG
BfrI 214 CTTAAG C^TTAAG
BpuAI 1531 GAAGAC GAAGAC(2/6)
BsaAI 175 CACGTG [CT]AC^GT[GA]
BsaI 1243 GAGACC GGTCTC(1/5)
BsiEI 1619 CGGCCG CG[GA][CT]^CG
BsiHKAI 178 GTGCTC G[AT]GC[AT]^C
BsmI 609 GAATGC GAATGC(1/-1)
BspDI 145 ATCGAT AT^CGAT
BspMI 47 ACCTGC ACCTGC(4/8)
ClaI 145 ATCGAT AT^CGAT
CpoI 125 CGGACCG CG^G[AT]CCG
DraIII 1210 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1619 CGGCCG C^GGCCG
EclXI 1619 CGGCCG C^GGCCG
Eco52I 1619 CGGCCG C^GGCCG
EcoNI 1318 CCTGGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HinfI 555 GAGTC G^A[ACGT]TC
KspI 1037 CCGCGG CCGC^GG
MaeI 1528 CTAG C^TAG
MaeII 176 ACGT A^CGT
MluNI 407 TGGCCA TGG^CCA
MscI 407 TGGCCA TGG^CCA
NcoI 68 CCATGG C^CATGG
PflFI 1006 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 68 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 555 GAGTC GAGTC(4/5)
PmaCI 175 CACGTG CAC^GTG
PmlI 175 CACGTG CAC^GTG
RsrII 125 CGGACCG CG^G[AT]CCG
SacII 1037 CCGCGG CCGC^GG
SapI 819 GCTCTTC GCTCTTC(1/4)
SgrAI 858 CGCCGGCG C[GA]^CCGG[CT]G
StuI 531 AGGCCT AGG^CCT
Tth111I 1006 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 68 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 227 CCACCAAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 816 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwI 744 GGATC GGATC(4/5)
967 GGATC
AlwNI 509 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
694 CAGCTACTG
BglI 486 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1072 GCCCCTGCGGC
BsrDI 1266 CATTGC GCAATG(2/0)
848 GCAATG
BstXI 230 CCAAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
410 CCAATGGGCTGG
DdeI 440 CTGAG C^T[ACGT]AG
1241 CTGAG
EarI 571 CTCTTC CTCTTC(1/4)
820 CTCTTC
HphI 824 TCACC GGTGA(8/7)
1306 TCACC
Ksp632I 571 CTCTTC CTCTTC(1/4)
820 CTCTTC
MseI 215 TTAA T^TAA
1733 TTAA
PstI 506 CTGCAG CTGCA^G
1361 CTGCAG
SfcI 506 CTGCAG C^T[GA][CT]AG
1361 CTGCAG
SmaI 199 CCCGGG CCC^GGG
1592 CCCGGG
Tru9I 215 TTAA T^TAA
1733 TTAA
XmaCI 199 CCCGGG C^CCGGG
1592 CCCGGG
XmaI 199 CCCGGG C^CCGGG
1592 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
ApaI AvaI AvaII BanI BanII
BbeI BbvI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII ItaI
KasI MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfoI SmlI
StyI TaqI TseI TspRI TthHB8I