Restriction map for
pF1KE1085
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BamHI
BcgI BclI BfaI BglII BlnI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Bsu36I ClaI DraI DraIII
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI MaeI
MfeI MflI MroI MunI NcoI
NheI NotI NsiI NspV PacI
PmeI PshAI PshBI Psp1406I PvuI
SalI ScaI SfiI SfuI SnaBI
SpeI SspBI SspI SwaI Tsp509I
XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 243 GACGTC GACGT^C
AflII 688 CTTAAG C^TTAAG
AflIII 180 ACGCGT A^C[GA][CT]GT
AgeI 528 ACCGGT A^CCGGT
AspI 2257 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1042 GGCGCC GGCGC^C
BciVI 981 GGATAC GTATCC(6/5)
BfrI 688 CTTAAG C^TTAAG
BlpI 1767 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1767 GCTAAGC GC^T[ACGT]AGC
BsaBI 1212 GATTAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 400 CGTCTC CGTCTC(1/5)
BspHI 1445 TCATGA T^CATGA
BsrBI 1837 GAGCGG CCGCTC(-3/-3)
BsrDI 1102 GCAATG GCAATG(2/0)
BstXI 425 CCACCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1767 GCTAAGC GC^T[ACGT]AGC
CpoI 971 CGGTCCG CG^G[AT]CCG
KasI 1042 GGCGCC G^GCGCC
MamI 1212 GATTAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 180 ACGCGT A^CGCGT
MseI 689 TTAA T^TAA
NarI 1042 GGCGCC GG^CGCC
NdeI 1198 CATATG CA^TATG
NruI 2409 TCGCGA TCG^CGA
NspI 830 GCATGC [GA]CATG^[CT]
PflFI 2257 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 528 ACCGGT A^CCGGT
RcaI 1445 TCATGA T^CATGA
RsrII 971 CGGTCCG CG^G[AT]CCG
SbfI 1645 CCTGCAGG CCTGCA^GG
SexAI 1931 ACCTGGT A^CC[AT]GGT
SfoI 1042 GGCGCC GGC^GCC
SmaI 1287 CCCGGG CCC^GGG
SphI 830 GCATGC GCATG^C
Sse8387I 1645 CCTGCAGG CCTGCA^GG
Tru9I 689 TTAA T^TAA
Tth111I 2257 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2033 CCAACTTCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1287 CCCGGG C^CCGGG
XmaI 1287 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 243 GACGTC G[GA]^CG[CT]C
1042 GGCGCC
BbrPI 16 CACGTG CAC^GTG
559 CACGTG
BbsI 2139 GAAGAC GAAGAC(2/6)
1275 GTCTTC
BbvCI 1089 GCTGAGG CCTCAGC(-5/-2)
1720 GCTGAGG
BpuAI 2139 GAAGAC GAAGAC(2/6)
1275 GTCTTC
BsaHI 243 GACGTC G[GA]^CG[CT]C
1042 GGCGCC
BsaWI 528 ACCGGT [AT]^CCGG[AT]
1484 ACCGGA
BsiEI 309 CGGCCG CG[GA][CT]^CG
455 CGGCCG
BspMI 60 ACCTGC ACCTGC(4/8)
612 GCAGGT
BstAPI 900 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2049 GCAGCAGCTGC
EagI 309 CGGCCG C^GGCCG
455 CGGCCG
EclXI 309 CGGCCG C^GGCCG
455 CGGCCG
Eco52I 309 CGGCCG C^GGCCG
455 CGGCCG
Hin1I 243 GACGTC G[GA]^CG[CT]C
1042 GGCGCC
KspI 348 CCGCGG CCGC^GG
364 CCGCGG
MslI 525 CACACCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1860 CACCACAGTG
NaeI 457 GCCGGC GCC^GGC
1673 GCCGGC
NgoMIV 457 GCCGGC G^CCGGC
1673 GCCGGC
PaeR7I 403 CTCGAG C^TCGAG
1694 CTCGAG
PmaCI 16 CACGTG CAC^GTG
559 CACGTG
PmlI 16 CACGTG CAC^GTG
559 CACGTG
SacI 210 GAGCTC GAGCT^C
739 GAGCTC
SacII 348 CCGCGG CCGC^GG
364 CCGCGG
SapI 1323 GAAGAGC GCTCTTC(1/4)
2172 GCTCTTC
SgrAI 527 CACCGGTG C[GA]^CCGG[CT]G
1413 CACCGGCG
TfiI 1270 GAATC G^A[AT]TC
1699 GAATC
XhoI 403 CTCGAG C^TCGAG
1694 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BglI BmyI BpmI BsaAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I