Restriction map for
pF1KB7330
##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AclI AflII AgeI Alw44I AocI
Aor51HI ApaLI AseI AsnI AspI
AvrII BalI BamHI BcgI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsiWI BsmI BspDI BspHI BspLU11I
BstBI Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eco47III Eco81I
EcoRI EcoRV EcoT22I FseI HindIII
HpaI KspI MfeI MluI MluNI
MscI MunI NdeI NheI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SexAI SfiI
SfuI SnaBI SpeI SphI SspI
SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 1696 GACGTC GACGT^C
Acc65I 458 GGTACC G^GTACC
AccIII 590 TCCGGA T^CCGGA
AcsI 234 GAATTT [GA]^AATT[CT]
AhdI 1729 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 610 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 234 GAATTT [GA]^AATT[CT]
AscI 88 GGCGCGCC GG^CGCGCC
Asp718I 458 GGTACC G^GTACC
AspEI 1729 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2219 TGCGCA TGC^GCA
BbrPI 1074 CACGTG CAC^GTG
BclI 815 TGATCA T^GATCA
BfaI 1298 CTAG C^TAG
BglI 1337 GCCCCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1960 GAGACC GGTCTC(1/5)
BsaWI 590 TCCGGA [AT]^CCGG[AT]
BseAI 590 TCCGGA T^CCGGA
BseRI 2386 GAGGAG GAGGAG(10/8)
BsmBI 1238 CGTCTC CGTCTC(1/5)
Bsp1407I 1112 TGTACA T^GTACA
BspEI 590 TCCGGA T^CCGGA
BsrGI 1112 TGTACA T^GTACA
BssHII 89 GCGCGC G^CGCGC
Bst1107I 28 GTATAC GTA^TAC
BstXI 1850 CCAGCATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 431 GGATCT [GA]^GATC[CT]
BstZ17I 28 GTATAC GTA^TAC
Eam1105I 1729 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2295 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 815 TGATCA T^GATCA
FspI 2219 TGCGCA TGC^GCA
HincII 2038 GTCGAC GT[CT]^[GA]AC
HindII 2038 GTCGAC GT[CT]^[GA]AC
KpnI 458 GGTACC GGTAC^C
MaeI 1298 CTAG C^TAG
MflI 431 GGATCT [GA]^GATC[CT]
MroI 590 TCCGGA T^CCGGA
MseI 1745 TTAA T^TAA
NcoI 1895 CCATGG C^CATGG
NotI 258 GCGGCCGC GC^GGCCGC
PaeR7I 1417 CTCGAG C^TCGAG
PmaCI 1074 CACGTG CAC^GTG
PmlI 1074 CACGTG CAC^GTG
SacI 360 GAGCTC GAGCT^C
SalI 2038 GTCGAC G^TCGAC
SapI 357 GAAGAGC GCTCTTC(1/4)
SbfI 922 CCTGCAGG CCTGCA^GG
SgrAI 2568 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 922 CCTGCAGG CCTGCA^GG
SspBI 1112 TGTACA T^GTACA
Tru9I 1745 TTAA T^TAA
XhoI 1417 CTCGAG C^TCGAG
XhoII 431 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 28 GTATAC GT^[AC][GT]AC
2038 GTCGAC
AflIII 293 ACGTGT A^C[GA][CT]GT
2354 ACACGT
Asp700I 495 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2094 GAAGAACTTC
AspHI 360 GAGCTC G[AT]GC[AT]^C
523 GTGCTC
BbsI 2020 GAAGAC GAAGAC(2/6)
1327 GTCTTC
BbvCI 159 CCTCAGC CCTCAGC(-5/-2)
1179 GCTGAGG
BpuAI 2020 GAAGAC GAAGAC(2/6)
1327 GTCTTC
BsaAI 292 TACGTG [CT]AC^GT[GA]
1074 CACGTG
BsaBI 510 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1941 GATGGGCATC
BsiHKAI 360 GAGCTC G[AT]GC[AT]^C
523 GTGCTC
BsrDI 537 CATTGC GCAATG(2/0)
2499 CATTGC
BstAPI 609 GCAGATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1253 GCAGCCCGTGC
BstEII 1098 GGTCACC G^GT[ACGT]ACC
1394 GGTCACC
BstPI 1098 GGTCACC G^GT[ACGT]ACC
1394 GGTCACC
EarI 357 GAAGAG CTCTTC(1/4)
990 GAAGAG
EcoO65I 1098 GGTCACC G^GT[ACGT]ACC
1394 GGTCACC
HgaI 1292 GACGC GACGC(5/10)
1504 GCGTC
Ksp632I 357 GAAGAG CTCTTC(1/4)
990 GAAGAG
MamI 510 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1941 GATGGGCATC
PvuII 1168 CAGCTG CAG^CTG
2454 CAGCTG
StuI 1011 AGGCCT AGG^CCT
1967 AGGCCT
TfiI 612 GATTC G^A[AT]TC
1034 GATTC
Tsp509I 235 AATT ^AATT
1743 AATT
TspRI 699 CACTG CA[GC]TG[ACGT][ACGT]^
2131 CAGTG
XmnI 495 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2094 GAAGAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AvaI AvaII BanI
BanII BbeI BbvI BcnI BmyI
BpmI BsaHI BsaJI BsgI BsiEI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SfoI
SmaI SmlI StyI TaqI TseI
Tsp45I TthHB8I Van91I XcmI XmaCI
XmaI