##################################
# NO CUT ENZYMES (  195 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AlwI        AlwNI       AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbsI        BbvCI       BcgI        BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DpnII       DraI        DraIII      DrdI        
DsaI        EaeI        EagI        EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FokI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       HphI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MamI        MboI        MboII       MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MspA1I      MspI        MunI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmeI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tru9I       Tth111I     TthHB8I     Van91I      XbaI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcsI          276  AAATTC               [GA]^AATT[CT]
AhdI           54  GACTAAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI          120  AGCT                 AG^CT
Alw44I        247  GTGCAC               G^TGCAC
ApaLI         247  GTGCAC               G^TGCAC
ApoI          276  AAATTC               [GA]^AATT[CT]
AspEI          54  GACTAAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         247  GTGCAC               G[AT]GC[AT]^C
AvaII          85  GGACC                G^G[AT]CC
BbrPI          89  CACGTG               CAC^GTG
BbvI          118  GCAGC                GCAGC(8/12)
BciVI         105  GGATAC               GTATCC(6/5)
BmyI          247  GTGCAC               G[AGT]GC[ACT]^C
BsgI          343  GTGCAG               GTGCAG(16/14)
BsiHKAI       247  GTGCAC               G[AT]GC[AT]^C
Bsp1286I      247  GTGCAC               G[AGT]GC[ACT]^C
BspMI         287  GCAGGT               ACCTGC(4/8)
BsrDI         136  GCAATG               GCAATG(2/0)
BsrI           65  CCAGT                ACTGG(1/-1)
Cac8I         161  GCATGC               GC[ACGT]^[ACGT]GC
DraII         435  AGGCCCC              [GA]G^G[ACGT]CC[CT]
Eam1105I       54  GACTAAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I      435  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoT14I        37  CCAAGG               C^C[AT][AT]GG
Fnu4HI        118  GCAGC                GC^[ACGT]GC
HaeIII        436  GGCC                 GG^CC
HinfI         400  GATTC                G^A[ACGT]TC
ItaI          118  GCAGC                GC^[ACGT]GC
MslI          369  CACAAAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PmaCI          89  CACGTG               CAC^GTG
PmlI           89  CACGTG               CAC^GTG
PstI          149  CTGCAG               CTGCA^G
SphI          161  GCATGC               GCATG^C
StyI           37  CCAAGG               C^C[AT][AT]GG
TfiI          400  GATTC                G^A[AT]TC
TseI          118  GCAGC                G^C[AT]GC
Tsp45I        367  GTCAC                ^GT[GC]AC
TspRI          66  CAGTG                CA[GC]TG[ACGT][ACGT]^
XcmI           81  CCAGGGACCACGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

BsmAI         176  GTCTC                GTCTC(1/5)
              378  GTCTC               
BssKI          81  CCAGG                ^CC[ACGT]GG
              157  CCAGG               
BstNI          81  CCAGG                CC^[AT]GG
              157  CCAGG               
Cfr13I         85  GGACC                G^G[ACGT]CC
              436  GGCCC               
DdeI           50  CTCAG                C^T[ACGT]AG
               56  CTAAG               
EcoRII         81  CCAGG                ^CC[AT]GG
              157  CCAGG               
MvaI           81  CCAGG                CC^[AT]GG
              157  CCAGG               
Sau96I         85  GGACC                G^G[ACGT]CC
              436  GGCCC               
ScrFI          81  CCAGG                CC^[ACGT]GG
              157  CCAGG               
SfcI          149  CTGCAG               C^T[GA][CT]AG
              362  CTACAG              


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AfaI        BsaAI       BsaJI       BsiYI       BslI        
BsmFI       MaeII       MaeIII      MnlI        NlaIII      
NlaIV       NspI        RsaI        Tsp509I