Restriction map for
pF1KA0984
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflIII AgeI AlwI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspI AvaI AviII AvrII BamHI
BbrPI BbvCI BclI BlnI BsaAI
BsaBI BsiWI BsoBI BspDI BspHI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I ClaI CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeII MamI MluI MslI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I RcaI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccIII 1569 TCCGGA T^CCGGA
AcyI 638 GGCGCC G[GA]^CG[CT]C
AflII 1327 CTTAAG C^TTAAG
AhdI 1392 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 552 CCTAAGG CC^T[ACGT]AGG
AspEI 1392 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 99 TGGCCA TGG^CCA
BanI 638 GGCGCC G^G[CT][GA]CC
BbeI 638 GGCGCC GGCGC^C
BciVI 384 GGATAC GTATCC(6/5)
BfaI 658 CTAG C^TAG
BfrI 1327 CTTAAG C^TTAAG
BglI 1529 GCCCTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 109 GCTGAGC GC^T[ACGT]AGC
BpmI 1202 CTCCAG CTGGAG(16/14)
Bpu1102I 109 GCTGAGC GC^T[ACGT]AGC
BsaHI 638 GGCGCC G[GA]^CG[CT]C
BsaI 341 GAGACC GGTCTC(1/5)
BsaWI 1569 TCCGGA [AT]^CCGG[AT]
BseAI 1569 TCCGGA T^CCGGA
BseRI 958 GAGGAG GAGGAG(10/8)
BsgI 203 CTGCAC GTGCAG(16/14)
BsiEI 1553 CGATCG CG[GA][CT]^CG
BsmBI 1184 CGTCTC CGTCTC(1/5)
BspEI 1569 TCCGGA T^CCGGA
BspMI 1128 GCAGGT ACCTGC(4/8)
BsrFI 1575 GCCGGT [GA]^CCGG[CT]
Bsu36I 552 CCTAAGG CC^T[ACGT]AGG
CelII 109 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1575 GCCGGT [GA]^CCGG[CT]
DraI 507 TTTAAA TTT^AAA
DraII 65 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 99 TGGCCA [CT]^GGCC[GA]
Eam1105I 1392 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 552 CCTAAGG CC^T[ACGT]AGG
EcoNI 642 CCTTACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 65 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 1075 GATATC GAT^ATC
FauI 135 GCGGG CCCGC(4/6)
HaeII 638 GGCGCC [GA]GCGC^[CT]
HgaI 1799 GCGTC GACGC(5/10)
Hin1I 638 GGCGCC G[GA]^CG[CT]C
HphI 72 TCACC GGTGA(8/7)
KasI 638 GGCGCC G^GCGCC
MaeI 658 CTAG C^TAG
MfeI 730 CAATTG C^AATTG
MluNI 99 TGGCCA TGG^CCA
MroI 1569 TCCGGA T^CCGGA
MscI 99 TGGCCA TGG^CCA
MunI 730 CAATTG C^AATTG
NarI 638 GGCGCC GG^CGCC
PpuMI 65 AGGACCT [GA]G^G[AT]CC[CT]
PvuI 1553 CGATCG CGAT^CG
SacI 1669 GAGCTC GAGCT^C
ScaI 1097 AGTACT AGT^ACT
SfoI 638 GGCGCC GGC^GCC
SmlI 1327 CTTAAG C^T[CT][GA]AG
TfiI 520 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 50 GAATTC [GA]^AATT[CT]
619 GAATTC
ApoI 50 GAATTC [GA]^AATT[CT]
619 GAATTC
BcgI 855 CGATGAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1095 GCAGTACTCTCG
BglII 512 AGATCT A^GATCT
973 AGATCT
BsmFI 1415 GGGAC GGGAC(10/14)
1312 GTCCC
Bsp1407I 1142 TGTACA T^GTACA
1498 TGTACA
BsrDI 984 CATTGC GCAATG(2/0)
230 GCAATG
BsrGI 1142 TGTACA T^GTACA
1498 TGTACA
BsrI 1165 CCAGT ACTGG(1/-1)
1204 CCAGT
BstYI 512 AGATCT [GA]^GATC[CT]
973 AGATCT
CfoI 3 GCGC GCG^C
639 GCGC
EarI 389 CTCTTC CTCTTC(1/4)
838 GAAGAG
EcoRI 50 GAATTC G^AATTC
619 GAATTC
HhaI 3 GCGC GCG^C
639 GCGC
HinP1I 3 GCGC G^CGC
639 GCGC
Ksp632I 389 CTCTTC CTCTTC(1/4)
838 GAAGAG
MaeIII 1168 GTGAC ^GT[ACGT]AC
1286 GTCAC
MflI 512 AGATCT [GA]^GATC[CT]
973 AGATCT
NspI 484 ACATGC [GA]CATG^[CT]
1382 ACATGC
PvuII 1508 CAGCTG CAG^CTG
1658 CAGCTG
SspBI 1142 TGTACA T^GTACA
1498 TGTACA
Tsp45I 1168 GTGAC ^GT[GC]AC
1286 GTCAC
XhoII 512 AGATCT [GA]^GATC[CT]
973 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI Alw44I AlwNI
ApaLI AspHI AvaII BanII BbsI
BbvI BcnI BmyI BpuAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmI
Bsp1286I BssKI BstF5I BstNI BstXI
Cac8I Cfr13I DdeI DpnII DsaI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MboI
MboII MnlI MseI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I