Restriction map for
pF1KA1086
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# NO CUT ENZYMES ( 84 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Alw44I ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvrII BbvCI BclI
BfaI BfrI BglII BlnI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI DraIII Eam1105I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI MaeI
MfeI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmeI PshBI
Psp1406I PvuI RsrII SalI SapI
ScaI SexAI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI SwaI
TfiI Tth111I Van91I XbaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflIII 500 ACGCGT A^C[GA][CT]GT
Aor51HI 1050 AGCGCT AGC^GCT
Asp700I 912 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1218 TGCGCA TGC^GCA
BbeI 327 GGCGCC GGCGC^C
BbrPI 1336 CACGTG CAC^GTG
BcgI 1325 CGAAGGACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 310 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 310 GCTCAGC GC^T[ACGT]AGC
BsaAI 1336 CACGTG [CT]AC^GT[GA]
BsaBI 2247 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2534 GAATGC GAATGC(1/-1)
BspHI 425 TCATGA T^CATGA
BssSI 2692 CTCGTG CACGAG(-5/-1)
BstXI 122 CCACCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 310 GCTCAGC GC^T[ACGT]AGC
DrdI 1648 GACCGGCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1630 CGGCCG C^GGCCG
EclXI 1630 CGGCCG C^GGCCG
Eco47III 1050 AGCGCT AGC^GCT
Eco52I 1630 CGGCCG C^GGCCG
FspI 1218 TGCGCA TGC^GCA
KasI 327 GGCGCC G^GCGCC
KspI 1202 CCGCGG CCGC^GG
MamI 2247 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 500 ACGCGT A^CGCGT
MseI 1319 TTAA T^TAA
MslI 123 CACCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 327 GGCGCC GG^CGCC
PmaCI 1336 CACGTG CAC^GTG
PmlI 1336 CACGTG CAC^GTG
RcaI 425 TCATGA T^CATGA
SacI 1696 GAGCTC GAGCT^C
SacII 1202 CCGCGG CCGC^GG
SbfI 595 CCTGCAGG CCTGCA^GG
SfiI 1071 GGCCTCGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 327 GGCGCC GGC^GCC
Sse8387I 595 CCTGCAGG CCTGCA^GG
Tru9I 1319 TTAA T^TAA
Tsp509I 1154 AATT ^AATT
XmnI 912 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccIII 343 TCCGGA T^CCGGA
2612 TCCGGA
AocI 2134 CCTCAGG CC^T[ACGT]AGG
2262 CCTGAGG
BamHI 898 GGATCC G^GATCC
2442 GGATCC
BciVI 55 GGATAC GTATCC(6/5)
373 GGATAC
BseAI 343 TCCGGA T^CCGGA
2612 TCCGGA
BsiEI 1630 CGGCCG CG[GA][CT]^CG
1647 CGACCG
BspEI 343 TCCGGA T^CCGGA
2612 TCCGGA
BsrDI 838 CATTGC GCAATG(2/0)
1395 CATTGC
BstEII 1986 GGTGACC G^GT[ACGT]ACC
2067 GGTCACC
BstPI 1986 GGTGACC G^GT[ACGT]ACC
2067 GGTCACC
Bsu36I 2134 CCTCAGG CC^T[ACGT]AGG
2262 CCTGAGG
EarI 1792 GAAGAG CTCTTC(1/4)
2616 GAAGAG
Eco81I 2134 CCTCAGG CC^T[ACGT]AGG
2262 CCTGAGG
EcoO65I 1986 GGTGACC G^GT[ACGT]ACC
2067 GGTCACC
Ksp632I 1792 GAAGAG CTCTTC(1/4)
2616 GAAGAG
MroI 343 TCCGGA T^CCGGA
2612 TCCGGA
PshAI 393 GACAGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1827 GACTCGGGTC
StuI 1079 AGGCCT AGG^CCT
1872 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
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AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PaeR7I PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XcmI XhoI XhoII XmaCI XmaI