Restriction map for
pF1KA1432
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AviII BamHI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Cfr10I ClaI CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcyI 1307 GGCGCC G[GA]^CG[CT]C
AhdI 267 GACAACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1254 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 871 CCTAAGG CC^T[ACGT]AGG
AspEI 267 GACAACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 364 CCTAGG C^CTAGG
BbeI 1307 GGCGCC GGCGC^C
BglII 578 AGATCT A^GATCT
BlnI 364 CCTAGG C^CTAGG
BsaHI 1307 GGCGCC G[GA]^CG[CT]C
BsmI 1726 GAATGC GAATGC(1/-1)
BspLU11I 1271 ACATGT A^CATGT
BspMI 2157 GCAGGT ACCTGC(4/8)
BssSI 98 CTCGTG CACGAG(-5/-1)
BstAPI 630 GCAAGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1850 CCATGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 871 CCTAAGG CC^T[ACGT]AGG
CfoI 1308 GCGC GCG^C
DraI 2106 TTTAAA TTT^AAA
Eam1105I 267 GACAACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 871 CCTAAGG CC^T[ACGT]AGG
EcoNI 300 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 435 CCCGC CCCGC(4/6)
HaeII 1307 GGCGCC [GA]GCGC^[CT]
HhaI 1308 GCGC GCG^C
Hin1I 1307 GGCGCC G[GA]^CG[CT]C
HinP1I 1308 GCGC G^CGC
KasI 1307 GGCGCC G^GCGCC
NarI 1307 GGCGCC GG^CGCC
NheI 438 GCTAGC G^CTAGC
PflMI 2055 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 688 GAGCTC GAGCT^C
SalI 1028 GTCGAC G^TCGAC
ScaI 705 AGTACT AGT^ACT
SfoI 1307 GGCGCC GGC^GCC
SmlI 1576 CTTGAG C^T[CT][GA]AG
StuI 1953 AGGCCT AGG^CCT
Van91I 2055 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2265 CCAGGGTGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1028 GTCGAC GT^[AC][GT]AC
1414 GTAGAC
AflIII 271 ACGTGT A^C[GA][CT]GT
1271 ACATGT
Asp700I 1171 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2335 GAACCTTTTC
BalI 1462 TGGCCA TGG^CCA
1859 TGGCCA
BanI 1307 GGCGCC G^G[CT][GA]CC
1928 GGTGCC
BbsI 822 GAAGAC GAAGAC(2/6)
2007 GTCTTC
BlpI 318 GCTGAGC GC^T[ACGT]AGC
1623 GCTCAGC
Bpu1102I 318 GCTGAGC GC^T[ACGT]AGC
1623 GCTCAGC
BpuAI 822 GAAGAC GAAGAC(2/6)
2007 GTCTTC
BsgI 104 GTGCAG GTGCAG(16/14)
332 GTGCAG
BspHI 143 TCATGA T^CATGA
1647 TCATGA
BstYI 173 AGATCC [GA]^GATC[CT]
578 AGATCT
CelII 318 GCTGAGC GC^T[ACGT]AGC
1623 GCTCAGC
EaeI 1462 TGGCCA [CT]^GGCC[GA]
1859 TGGCCA
HincII 602 GTCAAC GT[CT]^[GA]AC
1028 GTCGAC
HindII 602 GTCAAC GT[CT]^[GA]AC
1028 GTCGAC
MflI 173 AGATCC [GA]^GATC[CT]
578 AGATCT
MluNI 1462 TGGCCA TGG^CCA
1859 TGGCCA
MscI 1462 TGGCCA TGG^CCA
1859 TGGCCA
PleI 1442 GACTC GAGTC(4/5)
2199 GACTC
PpuMI 1019 GGGACCT [GA]G^G[AT]CC[CT]
1334 AGGACCT
PshAI 801 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1021 GACCTTTGTC
RcaI 143 TCATGA T^CATGA
1647 TCATGA
SapI 2152 GAAGAGC GCTCTTC(1/4)
543 GCTCTTC
SmaI 1409 CCCGGG CCC^GGG
2219 CCCGGG
XhoII 173 AGATCC [GA]^GATC[CT]
578 AGATCT
XmaCI 1409 CCCGGG C^CCGGG
2219 CCCGGG
XmaI 1409 CCCGGG C^CCGGG
2219 CCCGGG
XmnI 1171 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2335 GAACCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I