################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AhdI Alw44I AocI Aor51HI ApaLI AscI Asp718I AspEI AspI AviII BalI BbeI BbrPI BlpI Bpu1102I BsaAI BsaHI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI ClaI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoT22I FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI KspI MluI MluNI MroI MscI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI Psp1406I PvuI SacI SacII SalI ScaI SfiI SfoI SfuI SnaBI SphI SspBI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AgeI 150 ACCGGT A^CCGGT ApaI 1058 GGGCCC GGGCC^C AvrII 612 CCTAGG C^CTAGG BbvCI 32 CCTCAGC CCTCAGC(-5/-2) BclI 1971 TGATCA T^GATCA BglI 1735 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 612 CCTAGG C^CTAGG BsaBI 938 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 288 GAATGC GAATGC(1/-1) BspLU11I 881 ACATGT A^CATGT BsrFI 150 ACCGGT [GA]^CCGG[CT] BstXI 3786 CCAACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 150 ACCGGT [GA]^CCGG[CT] CpoI 4320 CGGACCG CG^G[AT]CCG EaeI 3723 TGGCCG [CT]^GGCC[GA] EcoNI 475 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2695 GAATTC G^AATTC EcoRV 3061 GATATC GAT^ATC FbaI 1971 TGATCA T^GATCA HgaI 1019 GCGTC GACGC(5/10) MaeII 526 ACGT A^CGT MamI 938 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2225 CAATTG C^AATTG MspA1I 592 CAGCTG C[AC]G^C[GT]G MunI 2225 CAATTG C^AATTG PinAI 150 ACCGGT A^CCGGT PshAI 1372 GACTGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 592 CAGCTG CAG^CTG RsrII 4320 CGGACCG CG^G[AT]CCG SbfI 382 CCTGCAGG CCTGCA^GG SexAI 1839 ACCAGGT A^CC[AT]GGT SgrAI 149 CACCGGTG C[GA]^CCGG[CT]G SmaI 2430 CCCGGG CCC^GGG SpeI 2482 ACTAGT A^CTAGT Sse8387I 382 CCTGCAGG CCTGCA^GG SspI 4104 AATATT AAT^ATT SwaI 2069 ATTTAAAT ATTT^AAAT XbaI 120 TCTAGA T^CTAGA XmaCI 2430 CCCGGG C^CCGGG XmaI 2430 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AflII 1261 CTTAAG C^TTAAG 3024 CTTAAG AflIII 526 ACGTGT A^C[GA][CT]GT 881 ACATGT AlwNI 533 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG 2084 CAGGCCCTG AvaI 1391 CTCGGG C^[CT]CG[GA]G 2430 CCCGGG BamHI 684 GGATCC G^GATCC 4047 GGATCC BcgI 261 CGAACATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 420 GCAGCCAAGTCG BciVI 177 GTATCC GTATCC(6/5) 3782 GTATCC BcnI 2430 CCCGG CC^[GC]GG 2431 CCGGG BfrI 1261 CTTAAG C^TTAAG 3024 CTTAAG BglII 1340 AGATCT A^GATCT 2918 AGATCT BsaI 166 GAGACC GGTCTC(1/5) 187 GGTCTC BsaWI 150 ACCGGT [AT]^CCGG[AT] 3219 ACCGGA BseRI 1413 CTCCTC GAGGAG(10/8) 1416 CTCCTC BsoBI 1391 CTCGGG C^[CT]CG[GA]G 2430 CCCGGG BspHI 2328 TCATGA T^CATGA 3998 TCATGA BstAPI 2335 GCAAAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2825 GCAGTAATTGC FauI 228 CCCGC CCCGC(4/6) 3647 CCCGC NciI 2430 CCCGG CC^[GC]GG 2431 CCGGG NdeI 1346 CATATG CA^TATG 3415 CATATG PflMI 90 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1234 CCATGCAGTGG RcaI 2328 TCATGA T^CATGA 3998 TCATGA SapI 2375 GCTCTTC GCTCTTC(1/4) 3569 GCTCTTC StuI 321 AGGCCT AGG^CCT 2007 AGGCCT Van91I 90 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1234 CCATGCAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AseI AsnI Asp700I AspHI AvaII BanI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PshBI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI