Restriction map for
pF1KE0309
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AlwNI AocI ApaI
AscI AseI AsnI Asp718I AspI
AviII AvrII BamHI BbrPI BciVI
BfrI BlnI BsaAI BsaBI BseAI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DrdI EagI EclXI
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MluI MroI MseI MslI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI Tru9I Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 769 GTAGAC GT^[AC][GT]AC
AcsI 142 AAATTC [GA]^AATT[CT]
AhdI 1597 GACGGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 37 GTGCAC G^TGCAC
Aor51HI 1397 AGCGCT AGC^GCT
ApaLI 37 GTGCAC G^TGCAC
ApoI 142 AAATTC [GA]^AATT[CT]
Asp700I 397 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1597 GACGGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 566 CTCGGG C^[CT]CG[GA]G
BalI 1559 TGGCCA TGG^CCA
BanII 1248 GAGCTC G[GA]GC[CT]^C
BbeI 1571 GGCGCC GGCGC^C
BbsI 544 GAAGAC GAAGAC(2/6)
BclI 275 TGATCA T^GATCA
BfaI 62 CTAG C^TAG
BlpI 1047 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1047 GCTGAGC GC^T[ACGT]AGC
BpuAI 544 GAAGAC GAAGAC(2/6)
BsaWI 155 ACCGGA [AT]^CCGG[AT]
BsiEI 633 CGACCG CG[GA][CT]^CG
BsmBI 1216 GAGACG CGTCTC(1/5)
BsoBI 566 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1487 TGTACA T^GTACA
BsrGI 1487 TGTACA T^GTACA
BstAPI 104 GCAAGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1236 GGTGACC G^GT[ACGT]ACC
BstPI 1236 GGTGACC G^GT[ACGT]ACC
CelII 1047 GCTGAGC GC^T[ACGT]AGC
DraIII 496 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1597 GACGGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1397 AGCGCT AGC^GCT
EcoO65I 1236 GGTGACC G^GT[ACGT]ACC
FbaI 275 TGATCA T^GATCA
KasI 1571 GGCGCC G^GCGCC
MaeI 62 CTAG C^TAG
MfeI 939 CAATTG C^AATTG
MluNI 1559 TGGCCA TGG^CCA
MscI 1559 TGGCCA TGG^CCA
MunI 939 CAATTG C^AATTG
NarI 1571 GGCGCC GG^CGCC
NspI 1385 GCATGC [GA]CATG^[CT]
PflMI 938 CCAATTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 1248 GAGCTC GAGCT^C
SfoI 1571 GGCGCC GGC^GCC
SphI 1385 GCATGC GCATG^C
SspBI 1487 TGTACA T^GTACA
StuI 1061 AGGCCT AGG^CCT
Van91I 938 CCAATTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 397 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BbvCI 1188 CCTCAGC CCTCAGC(-5/-2)
1470 CCTCAGC
BglII 1151 AGATCT A^GATCT
1325 AGATCT
BpmI 25 CTGGAG CTGGAG(16/14)
265 CTGGAG
BsaI 1201 GAGACC GGTCTC(1/5)
1548 GGTCTC
BsgI 381 CTGCAC GTGCAG(16/14)
846 CTGCAC
BsrFI 806 GCCGGC [GA]^CCGG[CT]
1403 GCCGGC
Cfr10I 806 GCCGGC [GA]^CCGG[CT]
1403 GCCGGC
EarI 1182 CTCTTC CTCTTC(1/4)
520 GAAGAG
FauI 974 CCCGC CCCGC(4/6)
1445 GCGGG
HinfI 901 GATTC G^A[ACGT]TC
1254 GAATC
Ksp632I 1182 CTCTTC CTCTTC(1/4)
520 GAAGAG
NaeI 806 GCCGGC GCC^GGC
1403 GCCGGC
NcoI 1124 CCATGG C^CATGG
1667 CCATGG
NgoMIV 806 GCCGGC G^CCGGC
1403 GCCGGC
PpuMI 648 GGGTCCC [GA]G^G[AT]CC[CT]
761 GGGACCT
SexAI 734 ACCTGGT A^CC[AT]GGT
764 ACCTGGT
SmlI 124 CTCAAG C^T[CT][GA]AG
553 CTTGAG
TfiI 901 GATTC G^A[AT]TC
1254 GAATC
Tsp45I 118 GTGAC ^GT[GC]AC
1237 GTGAC
Tsp509I 143 AATT ^AATT
940 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaII BanI BbvI
BcgI BcnI BglI BmyI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
TspRI TthHB8I XhoII