Restriction map for
pF1KE0557
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BciVI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspEI BspLU11I BspMI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I Bsu36I CelII
Cfr10I CpoI DraI DraIII DrdI
EaeI EagI EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT14I EcoT22I
FseI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StyI SwaI Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 1285 GTAGAC GT^[AC][GT]AC
AccII 807 CGCG CG^CG
AhdI 1143 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1037 GGGCCC GGGCC^C
AspEI 1143 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 972 GTGCTC G[AT]GC[AT]^C
AvaI 730 CCCGGG C^[CT]CG[GA]G
BbvCI 878 CCTCAGC CCTCAGC(-5/-2)
BcgI 777 CGAAGCCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 378 TGATCA T^GATCA
BsiHKAI 972 GTGCTC G[AT]GC[AT]^C
BsoBI 730 CCCGGG C^[CT]CG[GA]G
BspDI 472 ATCGAT AT^CGAT
BspHI 381 TCATGA T^CATGA
BsrBI 211 GAGCGG CCGCTC(-3/-3)
BstAPI 675 GCAAGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 240 GGTGACC G^GT[ACGT]ACC
BstPI 240 GGTGACC G^GT[ACGT]ACC
BstUI 807 CGCG CG^CG
ClaI 472 ATCGAT AT^CGAT
DsaI 369 CCGTGG C^C[GA][CT]GG
Eam1105I 1143 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 627 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 240 GGTGACC G^GT[ACGT]ACC
FauI 213 GCGGG CCCGC(4/6)
FbaI 378 TGATCA T^GATCA
HaeII 832 AGCGCC [GA]GCGC^[CT]
HgaI 805 GACGC GACGC(5/10)
MslI 535 CACCAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 807 CGCG CG^CG
PpuMI 31 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 381 TCATGA T^CATGA
SexAI 1058 ACCAGGT A^CC[AT]GGT
SmaI 730 CCCGGG CCC^GGG
SphI 291 GCATGC GCATG^C
StuI 812 AGGCCT AGG^CCT
TfiI 612 GATTC G^A[AT]TC
Tsp45I 241 GTGAC ^GT[GC]AC
XmaCI 730 CCCGGG C^CCGGG
XmaI 730 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 31 enzymes) #
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AcsI 99 AAATTC [GA]^AATT[CT]
349 AAATTT
AfaI 92 GTAC GT^AC
774 GTAC
AlwNI 1084 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1102 CAGCTCCTG
ApoI 99 AAATTC [GA]^AATT[CT]
349 AAATTT
AvaII 32 GGTCC G^G[AT]CC
738 GGACC
AviII 688 TGCGCA TGC^GCA
1005 TGCGCA
BanII 217 GGGCTC G[GA]GC[CT]^C
1037 GGGCCC
BfaI 137 CTAG C^TAG
949 CTAG
BglII 603 AGATCT A^GATCT
851 AGATCT
BsgI 1054 CTGCAC GTGCAG(16/14)
594 GTGCAG
BsmFI 803 GGGAC GGGAC(10/14)
44 GTCCC
BsrDI 230 GCAATG GCAATG(2/0)
449 GCAATG
FspI 688 TGCGCA TGC^GCA
1005 TGCGCA
HapII 731 CCGG C^CGG
885 CCGG
HpaII 731 CCGG C^CGG
885 CCGG
HphI 240 GGTGA GGTGA(8/7)
534 TCACC
MaeI 137 CTAG C^TAG
949 CTAG
MaeII 704 ACGT A^CGT
710 ACGT
MseI 419 TTAA T^TAA
1265 TTAA
MspI 731 CCGG C^CGG
885 CCGG
NspI 291 GCATGC [GA]CATG^[CT]
761 ACATGC
PflMI 1083 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1320 CCACTCCCTGG
PleI 65 GACTC GAGTC(4/5)
506 GACTC
RsaI 92 GTAC GT^AC
774 GTAC
SapI 828 GAAGAGC GCTCTTC(1/4)
529 GCTCTTC
SmlI 508 CTCAAG C^T[CT][GA]AG
1276 CTTGAG
TaqI 9 TCGA T^CGA
473 TCGA
Tru9I 419 TTAA T^TAA
1265 TTAA
TthHB8I 9 TCGA T^CGA
473 TCGA
Van91I 1083 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1320 CCACTCCCTGG
XcmI 308 CCAACGCTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1250 CCAAAGCCATAGTGG
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# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AlwI BbvI BcnI
BmyI BpmI BsaI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HhaI HinP1I HinfI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MspA1I MvaI MwoI NciI
NdeII NlaIII NlaIV PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI Tsp509I TspRI XhoII