Restriction map for
pF1KE0560
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BbeI BbrPI BbsI BbvCI
BciVI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoT22I FauI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MluI MroI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 757 GTAGAC GT^[AC][GT]AC
ApaI 1058 GGGCCC GGGCC^C
AvaI 750 CCCGGG C^[CT]CG[GA]G
BalI 971 TGGCCA TGG^CCA
BamHI 22 GGATCC G^GATCC
BanI 190 GGTGCC G^G[CT][GA]CC
BcgI 798 CGAAGCCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 399 TGATCA T^GATCA
BfaI 1294 CTAG C^TAG
BglI 502 GCCAGACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 872 AGATCT A^GATCT
BsoBI 750 CCCGGG C^[CT]CG[GA]G
BspHI 402 TCATGA T^CATGA
BsrBI 788 GAGCGG CCGCTC(-3/-3)
BstAPI 82 GCAGGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 261 GGTGACC G^GT[ACGT]ACC
BstF5I 293 CATCC GGATG(2/0)
BstPI 261 GGTGACC G^GT[ACGT]ACC
BstXI 180 CCATGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 390 CCGTGG C^C[GA][CT]GG
EaeI 971 TGGCCA [CT]^GGCC[GA]
EcoO65I 261 GGTGACC G^GT[ACGT]ACC
EcoRI 1239 GAATTC G^AATTC
EcoRV 416 GATATC GAT^ATC
FbaI 399 TGATCA T^GATCA
FokI 293 CATCC GGATG(9/13)
HaeII 853 AGCGCC [GA]GCGC^[CT]
MaeI 1294 CTAG C^TAG
MaeIII 262 GTGAC ^GT[ACGT]AC
MfeI 428 CAATTG C^AATTG
MluNI 971 TGGCCA TGG^CCA
MscI 971 TGGCCA TGG^CCA
MunI 428 CAATTG C^AATTG
PflMI 1104 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 402 TCATGA T^CATGA
SexAI 1079 ACCAGGT A^CC[AT]GGT
SmaI 750 CCCGGG CCC^GGG
StuI 833 AGGCCT AGG^CCT
TaqI 1130 TCGA T^CGA
TfiI 1326 GATTC G^A[AT]TC
Tsp45I 262 GTGAC ^GT[GC]AC
TthHB8I 1130 TCGA T^CGA
Van91I 1104 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 750 CCCGGG C^CCGGG
XmaI 750 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AfaI 795 GTAC GT^AC
1025 GTAC
AflII 39 CTTAAG C^TTAAG
439 CTTAAG
BfrI 39 CTTAAG C^TTAAG
439 CTTAAG
BsgI 1075 CTGCAC GTGCAG(16/14)
80 GTGCAG
BsmFI 237 GGGAC GGGAC(10/14)
963 GTCCC
BsrI 507 ACTGG ACTGG(1/-1)
1269 ACTGG
DraII 1057 GGGGCCC [GA]G^G[ACGT]CC[CT]
1058 GGGCCCT
EcoO109I 1057 GGGGCCC [GA]G^G[ACGT]CC[CT]
1058 GGGCCCT
EcoT14I 143 CCTTGG C^C[AT][AT]GG
195 CCTTGG
HapII 751 CCGG C^CGG
906 CCGG
HgaI 609 GACGC GACGC(5/10)
826 GACGC
HpaII 751 CCGG C^CGG
906 CCGG
MslI 181 CATGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
918 CACCGAGATG
MspI 751 CCGG C^CGG
906 CCGG
RsaI 795 GTAC GT^AC
1025 GTAC
SacI 314 GAGCTC GAGCT^C
544 GAGCTC
SapI 849 GAAGAGC GCTCTTC(1/4)
1288 GAAGAGC
SfaNI 292 GCATC GCATC(5/9)
1122 GCATC
SmlI 39 CTTAAG C^T[CT][GA]AG
439 CTTAAG
StyI 143 CCTTGG C^C[AT][AT]GG
195 CCTTGG
XcmI 9 CCAATTTTCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1259 CCAAAACCACACTGG
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
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AccII AciI AcsI AluI AlwI
AlwNI ApoI AspHI AvaII BanII
BbvI BcnI BmyI BpmI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BssKI BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI HaeIII
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeII MboI MboII MflI
MnlI MseI MspA1I MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII Sau3AI
Sau96I ScrFI SfcI Tru9I TseI
Tsp509I TspRI XhoII