Restriction map for
pF1KB6133
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AvaI AviII BalI
BamHI BbeI BbrPI BcgI BclI
BfrI BlpI Bpu1102I BsaAI BsaHI
BsaI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspLU11I BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
ClaI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HaeII
Hin1I HindIII HpaI KasI KpnI
KspI MfeI MluI MluNI MscI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PmaCI PmeI PmlI PshBI
Psp1406I PvuI SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccII 505 CGCG CG^CG
AccIII 26 TCCGGA T^CCGGA
AcsI 1726 AAATTT [GA]^AATT[CT]
AgeI 254 ACCGGT A^CCGGT
AlwI 1176 GATCC GGATC(4/5)
AocI 675 CCTCAGG CC^T[ACGT]AGG
ApoI 1726 AAATTT [GA]^AATT[CT]
AspHI 1363 GAGCTC G[AT]GC[AT]^C
AspI 1201 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1623 CCTAGG C^CTAGG
BbsI 754 GAAGAC GAAGAC(2/6)
BbvCI 1018 GCTGAGG CCTCAGC(-5/-2)
BciVI 416 GTATCC GTATCC(6/5)
BglI 1118 GCCGGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1154 AGATCT A^GATCT
BlnI 1623 CCTAGG C^CTAGG
BpuAI 754 GAAGAC GAAGAC(2/6)
BsaBI 219 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 26 TCCGGA T^CCGGA
BsiHKAI 1363 GAGCTC G[AT]GC[AT]^C
BsmI 1432 GAATGC GAATGC(1/-1)
BspEI 26 TCCGGA T^CCGGA
BspMI 461 ACCTGC ACCTGC(4/8)
BsrBI 30 GAGCGG CCGCTC(-3/-3)
BsrDI 1239 CATTGC GCAATG(2/0)
BstUI 505 CGCG CG^CG
BstYI 1154 AGATCT [GA]^GATC[CT]
Bsu36I 675 CCTCAGG CC^T[ACGT]AGG
CpoI 109 CGGTCCG CG^G[AT]CCG
DraIII 591 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 977 CCATGG C^C[GA][CT]GG
EarI 1755 GAAGAG CTCTTC(1/4)
Eco81I 675 CCTCAGG CC^T[ACGT]AGG
EcoRV 219 GATATC GAT^ATC
Ksp632I 1755 GAAGAG CTCTTC(1/4)
MaeII 1391 ACGT A^CGT
MamI 219 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1154 AGATCT [GA]^GATC[CT]
MroI 26 TCCGGA T^CCGGA
MseI 899 TTAA T^TAA
MvnI 505 CGCG CG^CG
NaeI 1118 GCCGGC GCC^GGC
NcoI 977 CCATGG C^CATGG
NgoMIV 1118 GCCGGC G^CCGGC
PflFI 1201 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 254 ACCGGT A^CCGGT
PpuMI 743 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 945 GACGCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 109 CGGTCCG CG^G[AT]CCG
SacI 1363 GAGCTC GAGCT^C
SapI 1755 GAAGAGC GCTCTTC(1/4)
SexAI 746 ACCTGGT A^CC[AT]GGT
SmlI 1273 CTCAAG C^T[CT][GA]AG
SpeI 232 ACTAGT A^CTAGT
Tru9I 899 TTAA T^TAA
Tsp509I 1727 AATT ^AATT
Tth111I 1201 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1154 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Asp700I 909 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1755 GAAGAGCTTC
BanI 298 GGTGCC G^G[CT][GA]CC
619 GGCACC
BfaI 233 CTAG C^TAG
1624 CTAG
BsmFI 105 GTCCC GGGAC(10/14)
933 GTCCC
BspHI 905 TCATGA T^CATGA
1367 TCATGA
CfoI 504 GCGC GCG^C
1220 GCGC
DraII 743 AGGACCT [GA]G^G[ACGT]CC[CT]
1310 AGGCCCT
EcoO109I 743 AGGACCT [GA]G^G[ACGT]CC[CT]
1310 AGGCCCT
HgaI 945 GACGC GACGC(5/10)
1721 GCGTC
HhaI 504 GCGC GCG^C
1220 GCGC
HinP1I 504 GCGC G^CGC
1220 GCGC
HincII 433 GTCAAC GT[CT]^[GA]AC
1465 GTCAAC
HindII 433 GTCAAC GT[CT]^[GA]AC
1465 GTCAAC
MaeI 233 CTAG C^TAG
1624 CTAG
MaeIII 427 GTCAC ^GT[ACGT]AC
1480 GTCAC
MspA1I 685 CAGCTG C[AC]G^C[GT]G
1112 CAGCTG
NspI 737 ACATGC [GA]CATG^[CT]
948 GCATGT
PvuII 685 CAGCTG CAG^CTG
1112 CAGCTG
RcaI 905 TCATGA T^CATGA
1367 TCATGA
Tsp45I 427 GTCAC ^GT[GC]AC
1480 GTCAC
XmnI 909 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1755 GAAGAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BsaWI BseRI BsiYI BslI
BsmAI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI Cac8I Cfr10I Cfr13I
DdeI DpnII EaeI EcoNI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MboI MboII MnlI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TseI TspRI TthHB8I XcmI