Restriction map for
pF1KE0381
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI Alw44I ApaLI
ApoI AscI AseI AsnI Asp700I
AspI AviII AvrII BalI BamHI
BbrPI BbsI BcgI BciVI BclI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrGI
BssHII Bst1107I BstAPI BstBI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
EclXI Eco52I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KspI MamI MfeI
MluI MluNI MroI MscI MseI
MslI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AccII 559 CGCG CG^CG
AhdI 541 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1030 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1395 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1067 AGCGCT AGC^GCT
ApaI 1306 GGGCCC GGGCC^C
AspEI 541 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BsrBI 1479 GAGCGG CCGCTC(-3/-3)
BsrDI 1403 GCAATG GCAATG(2/0)
BssSI 736 CTCGTG CACGAG(-5/-1)
BstEII 81 GGTCACC G^GT[ACGT]ACC
BstPI 81 GGTCACC G^GT[ACGT]ACC
BstUI 559 CGCG CG^CG
Bsu36I 1395 CCTCAGG CC^T[ACGT]AGG
Eam1105I 541 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1067 AGCGCT AGC^GCT
Eco81I 1395 CCTCAGG CC^T[ACGT]AGG
EcoO65I 81 GGTCACC G^GT[ACGT]ACC
EcoT14I 153 CCTTGG C^C[AT][AT]GG
FauI 1481 GCGGG CCCGC(4/6)
MvnI 559 CGCG CG^CG
NaeI 113 GCCGGC GCC^GGC
NgoMIV 113 GCCGGC G^CCGGC
PshAI 810 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1279 GAAGAGC GCTCTTC(1/4)
SfiI 107 GGCCATGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 923 AGGCCT AGG^CCT
StyI 153 CCTTGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AlwI 1005 GATCC GGATC(4/5)
1026 GATCC
AvaI 118 CCCGGG C^[CT]CG[GA]G
194 CCCGGG
BbeI 1262 GGCGCC GGCGC^C
1339 GGCGCC
BbvCI 1380 CCTCAGC CCTCAGC(-5/-2)
1093 GCTGAGG
BfaI 568 CTAG C^TAG
1504 CTAG
BglI 108 GCCATGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1455 GCCCCAGGGGC
BglII 779 AGATCT A^GATCT
800 AGATCT
BsoBI 118 CCCGGG C^[CT]CG[GA]G
194 CCCGGG
BsrFI 113 GCCGGC [GA]^CCGG[CT]
938 GCCGGT
BstF5I 77 GGATG GGATG(2/0)
354 GGATG
Cfr10I 113 GCCGGC [GA]^CCGG[CT]
938 GCCGGT
DraII 131 GGGGCCT [GA]G^G[ACGT]CC[CT]
190 AGGCCCC
EarI 625 GAAGAG CTCTTC(1/4)
1279 GAAGAG
EcoO109I 131 GGGGCCT [GA]G^G[ACGT]CC[CT]
190 AGGCCCC
FokI 77 GGATG GGATG(9/13)
354 GGATG
HinfI 571 GAGTC G^A[ACGT]TC
956 GAGTC
KasI 1262 GGCGCC G^GCGCC
1339 GGCGCC
Ksp632I 625 GAAGAG CTCTTC(1/4)
1279 GAAGAG
MaeI 568 CTAG C^TAG
1504 CTAG
NarI 1262 GGCGCC GG^CGCC
1339 GGCGCC
PleI 571 GAGTC GAGTC(4/5)
956 GAGTC
PvuII 535 CAGCTG CAG^CTG
962 CAGCTG
SfoI 1262 GGCGCC GGC^GCC
1339 GGCGCC
SmaI 118 CCCGGG CCC^GGG
194 CCCGGG
SmlI 1078 CTTGAG C^T[CT][GA]AG
1198 CTCAAG
XmaCI 118 CCCGGG C^CCGGG
194 CCCGGG
XmaI 118 CCCGGG C^CCGGG
194 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
Acc65I AciI AcyI AfaI AluI
Asp718I AspHI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EcoNI
EcoRII Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI KpnI MaeII MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TseI Tsp45I TspRI TthHB8I
XcmI XhoII