##################################
# NO CUT ENZYMES (  214 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssKI       BssSI       
Bst1107I    BstBI       BstEII      BstNI       BstPI       
BstUI       BstXI       BstYI       BstZ17I     CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HaeIII      
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        HphI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MamI        MboII       MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MslI        MunI        MvaI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIII      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScaI        ScrFI       SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        TfiI        Tru9I       Tsp45I      Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   10 enzymes) #
###################################

AciI          362  GCGG                 CCGC(-3/-1)
AfaI          109  GTAC                 GT^AC
BsgI          384  GTGCAG               GTGCAG(16/14)
BsmFI         688  GTCCC                GGGAC(10/14)
DpnII         711  GATC                 ^GATC
MboI          711  GATC                 ^GATC
NdeII         711  GATC                 ^GATC
RsaI          109  GTAC                 GT^AC
Sau3AI        711  GATC                 ^GATC
Tsp509I       693  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

BanII         183  GGGCTC               G[GA]GC[CT]^C
              345  GGGCTC              
BstAPI        569  GCAAACCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              656  GCAAGCCCTGC         
HapII          77  CCGG                 C^CGG
               98  CCGG                
HpaII          77  CCGG                 C^CGG
               98  CCGG                
MspI           77  CCGG                 C^CGG
               98  CCGG                
TspRI         382  CAGTG                CA[GC]TG[ACGT][ACGT]^
              703  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AluI        AlwNI       AocI        BbvI        BmyI        
BpmI        BsaJI       BseRI       BsiYI       BslI        
Bsp1286I    BsrI        BstF5I      Bsu36I      Cac8I       
DdeI        Eco81I      EcoT14I     Fnu4HI      FokI        
ItaI        MnlI        MspA1I      MwoI        NlaIV       
PvuII       StyI        TseI