Restriction map for
pF1KB6327
##################################
# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
AspEI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HindIII HpaI KasI KspI
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MseI MspA1I
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp45I
Tsp509I XbaI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 617 GGTACC G^GTACC
ApaI 393 GGGCCC GGGCC^C
Asp700I 208 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 617 GGTACC G^GTACC
AspI 655 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 313 CCCGAG C^[CT]CG[GA]G
AvaII 342 GGACC G^G[AT]CC
BanI 617 GGTACC G^G[CT][GA]CC
BbsI 333 GAAGAC GAAGAC(2/6)
BciVI 36 GTATCC GTATCC(6/5)
BfaI 398 CTAG C^TAG
BglI 386 GCCGCATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 333 GAAGAC GAAGAC(2/6)
BsaAI 769 TACGTG [CT]AC^GT[GA]
BsgI 694 GTGCAG GTGCAG(16/14)
BsoBI 313 CCCGAG C^[CT]CG[GA]G
BspMI 100 ACCTGC ACCTGC(4/8)
BsrDI 91 CATTGC GCAATG(2/0)
DraII 393 GGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 679 GACACCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 761 CGGCCA [CT]^GGCC[GA]
EarI 381 GAAGAG CTCTTC(1/4)
EcoO109I 393 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 188 CCATGG C^C[AT][AT]GG
HgaI 426 GCGTC GACGC(5/10)
HincII 688 GTCAAC GT[CT]^[GA]AC
HindII 688 GTCAAC GT[CT]^[GA]AC
KpnI 617 GGTACC GGTAC^C
Ksp632I 381 GAAGAG CTCTTC(1/4)
MaeI 398 CTAG C^TAG
NaeI 759 GCCGGC GCC^GGC
NcoI 188 CCATGG C^CATGG
NgoMIV 759 GCCGGC G^CCGGC
NspI 82 GCATGC [GA]CATG^[CT]
PflFI 655 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 598 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 243 GAGTC GAGTC(4/5)
PstI 566 CTGCAG CTGCA^G
SapI 381 GAAGAGC GCTCTTC(1/4)
SfaNI 753 GATGC GCATC(5/9)
SgrAI 738 CACCGGCG C[GA]^CCGG[CT]G
SmlI 220 CTCAAG C^T[CT][GA]AG
SphI 82 GCATGC GCATG^C
StyI 188 CCATGG C^C[AT][AT]GG
TaqI 704 TCGA T^CGA
Tth111I 655 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 704 TCGA T^CGA
Van91I 598 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 540 CCAGTCCTATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 208 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 622 CGCG CG^CG
624 CGCG
AfaI 618 GTAC GT^AC
720 GTAC
AspHI 235 GAGCTC G[AT]GC[AT]^C
316 GAGCTC
BsaI 97 GAGACC GGTCTC(1/5)
660 GGTCTC
BseRI 262 GAGGAG GAGGAG(10/8)
370 GAGGAG
BsiHKAI 235 GAGCTC G[AT]GC[AT]^C
316 GAGCTC
BsmFI 677 GGGAC GGGAC(10/14)
724 GGGAC
BstUI 622 CGCG CG^CG
624 CGCG
BstXI 540 CCAGTCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
764 CCAACTACGTGG
DsaI 41 CCACGG C^C[GA][CT]GG
188 CCATGG
FauI 401 GCGGG CCCGC(4/6)
608 GCGGG
HaeII 592 AGCGCC [GA]GCGC^[CT]
640 AGCGCC
MaeII 692 ACGT A^CGT
770 ACGT
MslI 186 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
747 CACGGGGATG
MvnI 622 CGCG CG^CG
624 CGCG
RsaI 618 GTAC GT^AC
720 GTAC
SacI 235 GAGCTC GAGCT^C
316 GAGCTC
SfcI 566 CTGCAG C^T[GA][CT]AG
636 CTACAG
TfiI 326 GAATC G^A[AT]TC
433 GATTC
TspRI 113 CACTG CA[GC]TG[ACGT][ACGT]^
498 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI BanII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr10I
Cfr13I DpnII EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MboI MboII
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI TseI