Restriction map for
pF1KE0193
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AflIII AgeI AhdI AocI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BcgI BciVI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI MaeI
MamI MfeI MluI MluNI MroI
MscI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp45I Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 472 GTAGAC GT^[AC][GT]AC
AccII 772 CGCG CG^CG
AclI 138 AACGTT AA^CGTT
AcsI 448 AAATTT [GA]^AATT[CT]
Alw44I 337 GTGCAC G^TGCAC
AlwNI 422 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1067 AGCGCT AGC^GCT
ApaLI 337 GTGCAC G^TGCAC
ApoI 448 AAATTT [GA]^AATT[CT]
AvaI 59 CTCGGG C^[CT]CG[GA]G
BbvCI 582 CCTCAGC CCTCAGC(-5/-2)
BclI 591 TGATCA T^GATCA
BpmI 527 CTCCAG CTGGAG(16/14)
BsgI 630 CTGCAC GTGCAG(16/14)
BsmAI 320 GAGAC GTCTC(1/5)
BsoBI 59 CTCGGG C^[CT]CG[GA]G
BspMI 333 GCAGGT ACCTGC(4/8)
BsrBI 598 GAGCGG CCGCTC(-3/-3)
BsrDI 227 GCAATG GCAATG(2/0)
BstUI 772 CGCG CG^CG
BstYI 807 GGATCT [GA]^GATC[CT]
CfoI 1068 GCGC GCG^C
Eco47III 1067 AGCGCT AGC^GCT
FbaI 591 TGATCA T^GATCA
HaeII 1067 AGCGCT [GA]GCGC^[CT]
HhaI 1068 GCGC GCG^C
HinP1I 1068 GCGC G^CGC
KspI 771 CCGCGG CCGC^GG
MaeIII 78 GTTAC ^GT[ACGT]AC
MflI 807 GGATCT [GA]^GATC[CT]
MvnI 772 CGCG CG^CG
PflMI 183 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 15 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 138 AACGTT AA^CGTT
SacII 771 CCGCGG CCGC^GG
ScaI 146 AGTACT AGT^ACT
SfaNI 414 GATGC GCATC(5/9)
SfcI 614 CTACAG C^T[GA][CT]AG
Tsp509I 449 AATT ^AATT
Van91I 183 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 807 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 147 GTAC GT^AC
399 GTAC
AlwI 408 GGATC GGATC(4/5)
807 GGATC
AspHI 160 GAGCAC G[AT]GC[AT]^C
337 GTGCAC
AvaII 16 GGACC G^G[AT]CC
68 GGTCC
BcnI 300 CCGGG CC^[GC]GG
754 CCCGG
BseRI 792 CTCCTC GAGGAG(10/8)
490 GAGGAG
BsiHKAI 160 GAGCAC G[AT]GC[AT]^C
337 GTGCAC
BsmFI 15 GGGAC GGGAC(10/14)
737 GGGAC
BspHI 594 TCATGA T^CATGA
822 TCATGA
Cac8I 722 GCAAGC GC[ACGT]^[ACGT]GC
764 GCCTGC
DraII 15 GGGACCC [GA]G^G[ACGT]CC[CT]
423 AGGGCCT
EcoO109I 15 GGGACCC [GA]G^G[ACGT]CC[CT]
423 AGGGCCT
HapII 300 CCGG C^CGG
755 CCGG
HpaII 300 CCGG C^CGG
755 CCGG
MaeII 139 ACGT A^CGT
683 ACGT
MspA1I 538 CAGCGG C[AC]G^C[GT]G
771 CCGCGG
MspI 300 CCGG C^CGG
755 CCGG
NciI 300 CCGGG CC^[GC]GG
754 CCCGG
RcaI 594 TCATGA T^CATGA
822 TCATGA
RsaI 147 GTAC GT^AC
399 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI BanI BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII DsaI EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MboI MboII
MnlI MslI MvaI MwoI NcoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI StyI TaqI TfiI
TseI TspRI TthHB8I