Restriction map for
pF1KE0469
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
Alw44I AlwI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI ClaI CpoI DraI DraIII
DrdI EagI EarI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HhaI
HinP1I HincII HindII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MroI MslI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI TfiI Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcyI 62 GACGCC G[GA]^CG[CT]C
AfaI 405 GTAC GT^AC
AhdI 62 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 484 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 308 CCTTAGG CC^T[ACGT]AGG
AspEI 62 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 417 GGACC G^G[AT]CC
BanI 45 GGTGCC G^G[CT][GA]CC
BfaI 559 CTAG C^TAG
BglI 7 GCCCAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 452 AGATCT A^GATCT
BpmI 173 CTGGAG CTGGAG(16/14)
BsaHI 62 GACGCC G[GA]^CG[CT]C
BsmAI 281 GAGAC GTCTC(1/5)
BsmFI 290 GGGAC GGGAC(10/14)
Bsp1407I 404 TGTACA T^GTACA
BspMI 483 GCAGGT ACCTGC(4/8)
BsrFI 95 ACCGGC [GA]^CCGG[CT]
BsrGI 404 TGTACA T^GTACA
BstF5I 89 GGATG GGATG(2/0)
BstYI 452 AGATCT [GA]^GATC[CT]
Bsu36I 308 CCTTAGG CC^T[ACGT]AGG
Cac8I 126 GCTTGC GC[ACGT]^[ACGT]GC
Cfr10I 95 ACCGGC [GA]^CCGG[CT]
DpnII 453 GATC ^GATC
DsaI 614 CCGTGG C^C[GA][CT]GG
Eam1105I 62 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 308 CCTTAGG CC^T[ACGT]AGG
EcoNI 172 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 519 CCTTGG C^C[AT][AT]GG
FauI 231 GCGGG CCCGC(4/6)
FokI 89 GGATG GGATG(9/13)
HgaI 62 GACGC GACGC(5/10)
Hin1I 62 GACGCC G[GA]^CG[CT]C
HinfI 283 GACTC G^A[ACGT]TC
MaeI 559 CTAG C^TAG
MaeII 293 ACGT A^CGT
MboI 453 GATC ^GATC
MflI 452 AGATCT [GA]^GATC[CT]
MseI 587 TTAA T^TAA
NdeII 453 GATC ^GATC
PleI 283 GACTC GAGTC(4/5)
PstI 337 CTGCAG CTGCA^G
RsaI 405 GTAC GT^AC
Sau3AI 453 GATC ^GATC
SfiI 6 GGCCCAGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 404 TGTACA T^GTACA
StyI 519 CCTTGG C^C[AT][AT]GG
Tru9I 587 TTAA T^TAA
Tsp509I 550 AATT ^AATT
XcmI 267 CCAGGTGAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 452 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AciI 420 CCGC CCGC(-3/-1)
231 GCGG
AluI 381 AGCT AG^CT
440 AGCT
BalI 447 TGGCCA TGG^CCA
504 TGGCCA
BbsI 202 GAAGAC GAAGAC(2/6)
245 GTCTTC
BcnI 49 CCCGG CC^[GC]GG
390 CCGGG
BpuAI 202 GAAGAC GAAGAC(2/6)
245 GTCTTC
HindIII 380 AAGCTT A^AGCTT
439 AAGCTT
MaeIII 70 GTCAC ^GT[ACGT]AC
331 GTCAC
MluNI 447 TGGCCA TGG^CCA
504 TGGCCA
MscI 447 TGGCCA TGG^CCA
504 TGGCCA
NciI 49 CCCGG CC^[GC]GG
390 CCGGG
SfcI 239 CTGTAG C^T[GA][CT]AG
337 CTGCAG
Tsp45I 70 GTCAC ^GT[GC]AC
331 GTCAC
TspRI 198 CACTG CA[GC]TG[ACGT][ACGT]^
573 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
ApaI BanII BbvI BmyI BsaJI
BseRI BsiYI BslI Bsp1286I BsrI
BssKI BstNI Cfr13I DdeI DraII
EaeI EcoO109I EcoRII Fnu4HI HaeIII
HapII HpaII HphI ItaI MboII
MnlI MspI MvaI MwoI NlaIII
NlaIV Sau96I ScrFI SfaNI TseI