##################################
# NO CUT ENZYMES (  200 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvCI       BbvI        BcgI        BciVI       BfrI        
BglI        BglII       BlnI        BpmI        BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cac8I       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
Fnu4HI      FokI        FseI        FspI        HaeII       
HaeIII      HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
HphI        ItaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MspA1I      MunI        MvnI        MwoI        
NaeI        NarI        NcoI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScaI        
SexAI       SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        StyI        
TfiI        TseI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI           13  GTAGAC               GT^[AC][GT]AC
AciI            4  GCGG                 CCGC(-3/-1)
AcsI           83  GAATTT               [GA]^AATT[CT]
AluI          113  AGCT                 AG^CT
AlwI           54  GGATC                GGATC(4/5)
ApoI           83  GAATTT               [GA]^AATT[CT]
BanII          46  GAGCCC               G[GA]GC[CT]^C
BclI          138  TGATCA               T^GATCA
BcnI          261  CCGGG                CC^[GC]GG
BfaI           73  CTAG                 C^TAG
BlpI           66  GCTCAGC              GC^T[ACGT]AGC
BmyI           46  GAGCCC               G[AGT]GC[ACT]^C
Bpu1102I       66  GCTCAGC              GC^T[ACGT]AGC
BseRI          43  GAGGAG               GAGGAG(10/8)
Bsp1286I       46  GAGCCC               G[AGT]GC[ACT]^C
BstYI          54  GGATCT               [GA]^GATC[CT]
CelII          66  GCTCAGC              GC^T[ACGT]AGC
DraI          147  TTTAAA               TTT^AAA
EarI          214  GAAGAG               CTCTTC(1/4)
FbaI          138  TGATCA               T^GATCA
HapII         261  CCGG                 C^CGG
HpaII         261  CCGG                 C^CGG
Ksp632I       214  GAAGAG               CTCTTC(1/4)
MaeI           73  CTAG                 C^TAG
MaeII          11  ACGT                 A^CGT
MaeIII        181  GTGAC                ^GT[ACGT]AC
MflI           54  GGATCT               [GA]^GATC[CT]
MseI          148  TTAA                 T^TAA
MslI          130  CATGCTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          261  CCGG                 C^CGG
NciI          261  CCGGG                CC^[GC]GG
NdeI          209  CATATG               CA^TATG
NlaIII        130  CATG                 CATG^
NlaIV          45  GGAGCC               GG[ACGT]^[ACGT]CC
NspI          129  ACATGC               [GA]CATG^[CT]
SspI          241  AATATT               AAT^ATT
SwaI          146  ATTTAAAT             ATTT^AAAT
TaqI          117  TCGA                 T^CGA
Tru9I         148  TTAA                 T^TAA
Tsp45I        181  GTGAC                ^GT[GC]AC
TthHB8I       117  TCGA                 T^CGA
XhoII          54  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

BsaJI          50  CCCTGG               C^C[ACGT][ACGT]GG
              261  CCGGGG              
BstNI          51  CCTGG                CC^[AT]GG
              279  CCTGG               
DdeI           67  CTCAG                C^T[ACGT]AG
              295  CTGAG               
EcoRII         51  CCTGG                ^CC[AT]GG
              279  CCTGG               
MvaI           51  CCTGG                CC^[AT]GG
              279  CCTGG               
SfcI           38  CTGTAG               C^T[GA][CT]AG
              188  CTATAG              
Tsp509I        84  AATT                 ^AATT
              195  AATT                
TspRI          36  CACTG                CA[GC]TG[ACGT][ACGT]^
              293  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

BssKI       DpnII       MboI        MboII       MnlI        
NdeII       Sau3AI      ScrFI