################################## # NO CUT ENZYMES ( 210 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeII MamI MboII MfeI MflI MluI MroI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 21 enzymes) # ################################### AciI 237 CCGC CCGC(-3/-1) AlwI 358 GATCC GGATC(4/5) AvaII 45 GGTCC G^G[AT]CC BbvI 306 GCTGC GCAGC(8/12) BfaI 127 CTAG C^TAG BpmI 194 CTGGAG CTGGAG(16/14) BsiYI 48 CCAGGGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 48 CCAGGGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 197 GAGAC GTCTC(1/5) BssSI 173 CACGAG CACGAG(-5/-1) BstAPI 271 GCAGTTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr13I 45 GGTCC G^G[ACGT]CC HinfI 119 GAATC G^A[ACGT]TC MaeI 127 CTAG C^TAG MseI 275 TTAA T^TAA PstI 294 CTGCAG CTGCA^G Sau96I 45 GGTCC G^G[ACGT]CC SfaNI 325 GATGC GCATC(5/9) TfiI 119 GAATC G^A[AT]TC Tru9I 275 TTAA T^TAA TseI 306 GCTGC G^C[AT]GC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AluI 177 AGCT AG^CT 333 AGCT BalI 77 TGGCCA TGG^CCA 146 TGGCCA BsaJI 48 CCAGGG C^C[ACGT][ACGT]GG 264 CCCAGG BsrI 134 ACTGG ACTGG(1/-1) 242 ACTGG BssKI 48 CCAGG ^CC[ACGT]GG 265 CCAGG BstF5I 105 GGATG GGATG(2/0) 137 GGATG BstNI 48 CCAGG CC^[AT]GG 265 CCAGG DpnII 228 GATC ^GATC 358 GATC EaeI 77 TGGCCA [CT]^GGCC[GA] 146 TGGCCA EcoRII 48 CCAGG ^CC[AT]GG 265 CCAGG Fnu4HI 236 GCCGC GC^[ACGT]GC 306 GCTGC FokI 105 GGATG GGATG(9/13) 137 GGATG HaeIII 78 GGCC GG^CC 147 GGCC ItaI 236 GCCGC GC^[ACGT]GC 306 GCTGC MaeIII 40 GTTAC ^GT[ACGT]AC 164 GTTAC MboI 228 GATC ^GATC 358 GATC MluNI 77 TGGCCA TGG^CCA 146 TGGCCA MnlI 30 CCTC CCTC(7/6) 345 GAGG MscI 77 TGGCCA TGG^CCA 146 TGGCCA MvaI 48 CCAGG CC^[AT]GG 265 CCAGG MwoI 271 GCAGTTAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 376 GCATGGGTGGC NdeII 228 GATC ^GATC 358 GATC NlaIII 377 CATG CATG^ 386 CATG Sau3AI 228 GATC ^GATC 358 GATC ScrFI 48 CCAGG CC^[ACGT]GG 265 CCAGG TspRI 207 CACTG CA[GC]TG[ACGT][ACGT]^ 297 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 1 enzymes) # ######################################### SfcI