Restriction map for
pF1KA0705
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# NO CUT ENZYMES ( 64 enzymes) #
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AatII AclI AcsI AflIII AgeI
Alw44I ApaLI ApoI AscI Asp700I
AspI AviII AvrII BbrPI BglII
BlnI BsiWI BspHI BspLU11I BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CpoI DraI EcoO65I EcoRI FseI
FspI HpaI KspI MfeI MluI
MunI NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacII SalI SapI ScaI SfiI
SfuI SnaBI SpeI SphI SspI
SwaI Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 550 GGTACC G^GTACC
AccI 952 GTCTAC GT^[AC][GT]AC
AccIII 1368 TCCGGA T^CCGGA
AflII 1996 CTTAAG C^TTAAG
AhdI 1765 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3902 AGCGCT AGC^GCT
AseI 1426 ATTAAT AT^TAAT
AsnI 1426 ATTAAT AT^TAAT
Asp718I 550 GGTACC G^GTACC
AspEI 1765 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2895 TGGCCA TGG^CCA
BbvCI 1272 CCTCAGC CCTCAGC(-5/-2)
BcgI 378 GCAAATCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 254 TGATCA T^GATCA
BfrI 1996 CTTAAG C^TTAAG
BlpI 2390 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2390 GCTCAGC GC^T[ACGT]AGC
BsaAI 1740 CACGTA [CT]AC^GT[GA]
BsaBI 2290 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1368 TCCGGA T^CCGGA
BsmI 3437 GCATTC GAATGC(1/-1)
BspDI 1069 ATCGAT AT^CGAT
BspEI 1368 TCCGGA T^CCGGA
BsrBI 165 GAGCGG CCGCTC(-3/-3)
CelII 2390 GCTCAGC GC^T[ACGT]AGC
ClaI 1069 ATCGAT AT^CGAT
DraIII 2615 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1659 GACCTCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1765 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3902 AGCGCT AGC^GCT
EcoNI 129 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3817 GATATC GAT^ATC
EcoT22I 1247 ATGCAT ATGCA^T
FbaI 254 TGATCA T^GATCA
HindIII 9 AAGCTT A^AGCTT
KpnI 550 GGTACC GGTAC^C
MamI 2290 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2895 TGGCCA TGG^CCA
MroI 1368 TCCGGA T^CCGGA
MscI 2895 TGGCCA TGG^CCA
NsiI 1247 ATGCAT ATGCA^T
PaeR7I 3940 CTCGAG C^TCGAG
PflMI 1717 CCAACTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 319 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1426 ATTAAT AT^TAAT
SbfI 1203 CCTGCAGG CCTGCA^GG
SexAI 1508 ACCTGGT A^CC[AT]GGT
SgrAI 4115 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 1203 CCTGCAGG CCTGCA^GG
Van91I 1717 CCAACTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3940 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 13 enzymes) #
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ApaI 4200 GGGCCC GGGCC^C
4252 GGGCCC
BciVI 1743 GTATCC GTATCC(6/5)
2593 GTATCC
BsaI 211 GAGACC GGTCTC(1/5)
2873 GAGACC
BsaWI 1368 TCCGGA [AT]^CCGG[AT]
2876 ACCGGA
BsmBI 3723 CGTCTC CGTCTC(1/5)
1328 GAGACG
Bsp1407I 827 TGTACA T^GTACA
1959 TGTACA
BsrGI 827 TGTACA T^GTACA
1959 TGTACA
BstAPI 1707 GCACTCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2142 GCAGAACCTGC
BstXI 1051 CCATATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2711 CCAGTGATGTGG
NdeI 1052 CATATG CA^TATG
2225 CATATG
PvuII 79 CAGCTG CAG^CTG
3139 CAGCTG
SspBI 827 TGTACA T^GTACA
1959 TGTACA
XcmI 1179 CCACACAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3802 CCAGGCCCAACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI AspHI AvaI
AvaII BamHI BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PpuMI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI