Restriction map for
pF1KE0126
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# NO CUT ENZYMES ( 191 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AvaI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmFI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I Cac8I
CelII Cfr10I Cfr13I ClaI CpoI
DpnII DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
Hin1I HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MaeIII MamI
MboI MfeI MflI MluI MluNI
MroI MscI MseI MslI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NdeII NgoMIV NheI NlaIV
NotI NruI NsiI NspI PacI
PaeR7I PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SapI Sau3AI
Sau96I SbfI ScaI SexAI SfcI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StyI SwaI Tru9I Tsp45I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 30 CCGC CCGC(-3/-1)
Asp700I 12 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 184 GAGCTC G[AT]GC[AT]^C
AspI 166 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 184 GAGCTC G[GA]GC[CT]^C
BbsI 12 GAAGAC GAAGAC(2/6)
BcnI 93 CCCGG CC^[GC]GG
BmyI 184 GAGCTC G[AGT]GC[ACT]^C
BpuAI 12 GAAGAC GAAGAC(2/6)
BsaJI 344 CCCAGG C^C[ACGT][ACGT]GG
BsiHKAI 184 GAGCTC G[AT]GC[AT]^C
BsiYI 324 CCTGTACATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 324 CCTGTACATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 184 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 326 TGTACA T^GTACA
BsrGI 326 TGTACA T^GTACA
BstBI 37 TTCGAA TT^CGAA
BstNI 345 CCAGG CC^[AT]GG
DdeI 285 CTCAG C^T[ACGT]AG
EcoRII 345 CCAGG ^CC[AT]GG
HaeIII 231 GGCC GG^CC
HapII 94 CCGG C^CGG
HgaI 269 GCGTC GACGC(5/10)
HincII 172 GTCAAC GT[CT]^[GA]AC
HindII 172 GTCAAC GT[CT]^[GA]AC
HinfI 320 GATTC G^A[ACGT]TC
HpaII 94 CCGG C^CGG
MaeII 352 ACGT A^CGT
MspI 94 CCGG C^CGG
MvaI 345 CCAGG CC^[AT]GG
NciI 93 CCCGG CC^[GC]GG
NspV 37 TTCGAA TT^CGAA
PflFI 166 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 184 GAGCTC GAGCT^C
SfaNI 78 GCATC GCATC(5/9)
SfuI 37 TTCGAA TT^CGAA
SspBI 326 TGTACA T^GTACA
StuI 230 AGGCCT AGG^CCT
TfiI 320 GATTC G^A[AT]TC
TspRI 371 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 166 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 12 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AfaI 160 GTAC GT^AC
327 GTAC
BsmAI 349 GAGAC GTCTC(1/5)
271 GTCTC
BsmBI 270 CGTCTC CGTCTC(1/5)
349 GAGACG
BssKI 93 CCCGG ^CC[ACGT]GG
345 CCAGG
BstF5I 79 CATCC GGATG(2/0)
359 GGATG
CfoI 27 GCGC GCG^C
207 GCGC
FokI 79 CATCC GGATG(9/13)
359 GGATG
HaeII 26 AGCGCC [GA]GCGC^[CT]
206 GGCGCT
HhaI 27 GCGC GCG^C
207 GCGC
HinP1I 27 GCGC G^CGC
207 GCGC
MnlI 341 CCTC CCTC(7/6)
289 GAGG
RsaI 160 GTAC GT^AC
327 GTAC
ScrFI 93 CCCGG CC^[ACGT]GG
345 CCAGG
TaqI 38 TCGA T^CGA
69 TCGA
Tsp509I 198 AATT ^AATT
365 AATT
TthHB8I 38 TCGA T^CGA
69 TCGA
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
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AluI BbvI Fnu4HI HphI ItaI
MboII MwoI NlaIII TseI