Restriction map for
pF1KA0561
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# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AflII AhdI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BciVI BfrI BlnI
BsiWI BsmI Bsp1407I BspDI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI DrdI
Eam1105I EcoRI EcoT22I FseI FspI
HincII HindII HpaI KpnI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PmeI PshAI PshBI PvuI RsrII
SalI ScaI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI SwaI
Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AclI 2018 AACGTT AA^CGTT
AgeI 2105 ACCGGT A^CCGGT
AocI 868 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1172 AGCGCT AGC^GCT
BamHI 2275 GGATCC G^GATCC
BbrPI 520 CACGTG CAC^GTG
BclI 818 TGATCA T^GATCA
BglII 3233 AGATCT A^GATCT
BlpI 45 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 45 GCTGAGC GC^T[ACGT]AGC
BsaBI 819 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BstAPI 1286 GCATGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 434 GGTCACC G^GT[ACGT]ACC
BstPI 434 GGTCACC G^GT[ACGT]ACC
Bsu36I 868 CCTGAGG CC^T[ACGT]AGG
CelII 45 GCTGAGC GC^T[ACGT]AGC
DraIII 1894 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1172 AGCGCT AGC^GCT
Eco81I 868 CCTGAGG CC^T[ACGT]AGG
EcoO65I 434 GGTCACC G^GT[ACGT]ACC
EcoRV 3087 GATATC GAT^ATC
FbaI 818 TGATCA T^GATCA
HindIII 3499 AAGCTT A^AGCTT
MamI 819 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 929 TTAA T^TAA
NcoI 1694 CCATGG C^CATGG
NspI 1286 GCATGT [GA]CATG^[CT]
PaeR7I 2484 CTCGAG C^TCGAG
PflMI 882 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2105 ACCGGT A^CCGGT
PmaCI 520 CACGTG CAC^GTG
PmlI 520 CACGTG CAC^GTG
Psp1406I 2018 AACGTT AA^CGTT
SapI 3072 GAAGAGC GCTCTTC(1/4)
SbfI 2404 CCTGCAGG CCTGCA^GG
SfiI 2401 GGCCCTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 2484 CTCGAG C^T[CT][GA]AG
Sse8387I 2404 CCTGCAGG CCTGCA^GG
StuI 2566 AGGCCT AGG^CCT
Tru9I 929 TTAA T^TAA
Van91I 882 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2484 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AatII 2923 GACGTC GACGT^C
3052 GACGTC
AccIII 806 TCCGGA T^CCGGA
3280 TCCGGA
AflIII 521 ACGTGT A^C[GA][CT]GT
2008 ACACGT
BbsI 2185 GAAGAC GAAGAC(2/6)
2425 GTCTTC
BbvCI 898 GCTGAGG CCTCAGC(-5/-2)
2253 GCTGAGG
BfaI 718 CTAG C^TAG
2434 CTAG
BpuAI 2185 GAAGAC GAAGAC(2/6)
2425 GTCTTC
BsaAI 520 CACGTG [CT]AC^GT[GA]
1336 TACGTG
BseAI 806 TCCGGA T^CCGGA
3280 TCCGGA
BsiEI 429 CGGCCG CG[GA][CT]^CG
2580 CGGCCG
BspEI 806 TCCGGA T^CCGGA
3280 TCCGGA
BssSI 3722 CTCGTG CACGAG(-5/-1)
3742 CTCGTG
EagI 429 CGGCCG C^GGCCG
2580 CGGCCG
EclXI 429 CGGCCG C^GGCCG
2580 CGGCCG
Eco52I 429 CGGCCG C^GGCCG
2580 CGGCCG
KspI 27 CCGCGG CCGC^GG
3646 CCGCGG
MaeI 718 CTAG C^TAG
2434 CTAG
MroI 806 TCCGGA T^CCGGA
3280 TCCGGA
NaeI 2231 GCCGGC GCC^GGC
2578 GCCGGC
NgoMIV 2231 GCCGGC G^CCGGC
2578 GCCGGC
SacI 3426 GAGCTC GAGCT^C
3739 GAGCTC
SacII 27 CCGCGG CCGC^GG
3646 CCGCGG
SexAI 1145 ACCTGGT A^CC[AT]GGT
1843 ACCAGGT
TfiI 516 GAATC G^A[AT]TC
2065 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbvI BcgI BcnI BglI BmyI
BpmI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI