Restriction map for
pF1KB5387
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AscI
AseI AsnI Asp700I Asp718I AspEI
AvrII BbrPI BbsI BcgI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BseAI BsiWI
BsmBI BsmFI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MseI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Van91I XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 1171 CGCG CG^CG
AcsI 163 AAATTC [GA]^AATT[CT]
AcyI 663 GGCGCC G[GA]^CG[CT]C
Alw44I 1090 GTGCAC G^TGCAC
AlwNI 637 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 698 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1517 AGCGCT AGC^GCT
ApaI 767 GGGCCC GGGCC^C
ApaLI 1090 GTGCAC G^TGCAC
ApoI 163 AAATTC [GA]^AATT[CT]
AspI 277 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 569 CTCGAG C^[CT]CG[GA]G
AviII 314 TGCGCA TGC^GCA
BamHI 615 GGATCC G^GATCC
BbeI 663 GGCGCC GGCGC^C
BsaAI 1225 TACGTG [CT]AC^GT[GA]
BsaHI 663 GGCGCC G[GA]^CG[CT]C
BsaWI 1496 TCCGGT [AT]^CCGG[AT]
BsgI 998 CTGCAC GTGCAG(16/14)
BsmI 890 GCATTC GAATGC(1/-1)
BsoBI 569 CTCGAG C^[CT]CG[GA]G
BspHI 1145 TCATGA T^CATGA
BsrBI 114 CCGCTC CCGCTC(-3/-3)
BsrDI 876 CATTGC GCAATG(2/0)
BstUI 1171 CGCG CG^CG
BstXI 1310 CCACTGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 615 GGATCC [GA]^GATC[CT]
Bsu36I 698 CCTCAGG CC^T[ACGT]AGG
DsaI 1184 CCATGG C^C[GA][CT]GG
EarI 1400 CTCTTC CTCTTC(1/4)
Eco47III 1517 AGCGCT AGC^GCT
Eco81I 698 CCTCAGG CC^T[ACGT]AGG
FspI 314 TGCGCA TGC^GCA
HgaI 287 GCGTC GACGC(5/10)
Hin1I 663 GGCGCC G[GA]^CG[CT]C
KasI 663 GGCGCC G^GCGCC
Ksp632I 1400 CTCTTC CTCTTC(1/4)
MflI 615 GGATCC [GA]^GATC[CT]
MvnI 1171 CGCG CG^CG
NarI 663 GGCGCC GG^CGCC
NcoI 1184 CCATGG C^CATGG
PaeR7I 569 CTCGAG C^TCGAG
PflFI 277 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 136 CTGCAG CTGCA^G
PvuII 719 CAGCTG CAG^CTG
RcaI 1145 TCATGA T^CATGA
SfcI 136 CTGCAG C^T[GA][CT]AG
SfoI 663 GGCGCC GGC^GCC
SmlI 569 CTCGAG C^T[CT][GA]AG
Tth111I 277 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1370 CCATCAGGCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 569 CTCGAG C^TCGAG
XhoII 615 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 337 GTAC GT^AC
678 GTAC
BalI 8 TGGCCA TGG^CCA
626 TGGCCA
BbvCI 441 GCTGAGG CCTCAGC(-5/-2)
823 GCTGAGG
BclI 80 TGATCA T^GATCA
195 TGATCA
BsiEI 831 CGACCG CG[GA][CT]^CG
1047 CGACCG
BspMI 993 ACCTGC ACCTGC(4/8)
1593 GCAGGT
BssSI 847 CACGAG CACGAG(-5/-1)
1529 CTCGTG
DraII 766 GGGGCCC [GA]G^G[ACGT]CC[CT]
767 GGGCCCT
EaeI 8 TGGCCA [CT]^GGCC[GA]
626 TGGCCA
EcoO109I 766 GGGGCCC [GA]G^G[ACGT]CC[CT]
767 GGGCCCT
EcoT14I 881 CCAAGG C^C[AT][AT]GG
1184 CCATGG
FauI 911 CCCGC CCCGC(4/6)
1543 CCCGC
FbaI 80 TGATCA T^GATCA
195 TGATCA
HphI 428 TCACC GGTGA(8/7)
896 TCACC
MluNI 8 TGGCCA TGG^CCA
626 TGGCCA
MscI 8 TGGCCA TGG^CCA
626 TGGCCA
MspA1I 719 CAGCTG C[AC]G^C[GT]G
912 CCGCTG
NaeI 886 GCCGGC GCC^GGC
1103 GCCGGC
NgoMIV 886 GCCGGC G^CCGGC
1103 GCCGGC
RsaI 337 GTAC GT^AC
678 GTAC
SacI 424 GAGCTC GAGCT^C
1579 GAGCTC
SfaNI 180 GATGC GCATC(5/9)
1261 GATGC
StyI 881 CCAAGG C^C[AT][AT]GG
1184 CCATGG
TfiI 155 GATTC G^A[AT]TC
1032 GATTC
Tsp45I 23 GTGAC ^GT[GC]AC
289 GTCAC
Tsp509I 164 AATT ^AATT
450 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeI MaeII MaeIII
MboI MboII MnlI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
TaqI TseI TspRI TthHB8I