Restriction map for
pF1KB7888
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI AocI Aor51HI ApaI
AscI Asp718I AspEI AspI AviII
AvrII BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BlnI BpuAI
BsaAI BsaI BsiEI BsiWI BsmI
BspDI BspLU11I BsrBI BsrFI BssSI
Bst1107I BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI FbaI FseI FspI KpnI
KspI MluI NaeI NcoI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI SfiI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AccI 1946 GTCTAC GT^[AC][GT]AC
AccIII 1089 TCCGGA T^CCGGA
AcyI 214 GGCGCC G[GA]^CG[CT]C
Alw44I 1190 GTGCAC G^TGCAC
AlwNI 112 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1190 GTGCAC G^TGCAC
AseI 1252 ATTAAT AT^TAAT
AsnI 1252 ATTAAT AT^TAAT
AspHI 1190 GTGCAC G[AT]GC[AT]^C
AvaI 935 CTCGGG C^[CT]CG[GA]G
BalI 1200 TGGCCA TGG^CCA
BamHI 1368 GGATCC G^GATCC
BanII 159 GGGCTC G[GA]GC[CT]^C
BbeI 214 GGCGCC GGCGC^C
BglI 68 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1349 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1349 GCTTAGC GC^T[ACGT]AGC
BsaHI 214 GGCGCC G[GA]^CG[CT]C
BsaWI 1089 TCCGGA [AT]^CCGG[AT]
BseAI 1089 TCCGGA T^CCGGA
BsiHKAI 1190 GTGCAC G[AT]GC[AT]^C
BsmBI 82 GAGACG CGTCTC(1/5)
BsoBI 935 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1512 TGTACA T^GTACA
BspEI 1089 TCCGGA T^CCGGA
BspHI 1169 TCATGA T^CATGA
BsrDI 1083 GCAATG GCAATG(2/0)
BsrGI 1512 TGTACA T^GTACA
BssHII 12 GCGCGC G^CGCGC
BstAPI 1116 GCATAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 587 TTCGAA TT^CGAA
BstEII 805 GGTGACC G^GT[ACGT]ACC
BstPI 805 GGTGACC G^GT[ACGT]ACC
CelII 1349 GCTTAGC GC^T[ACGT]AGC
DraII 1224 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 970 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1217 GACTTTCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1224 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 805 GGTGACC G^GT[ACGT]ACC
EcoRV 438 GATATC GAT^ATC
EcoT22I 1694 ATGCAT ATGCA^T
HaeII 214 GGCGCC [GA]GCGC^[CT]
Hin1I 214 GGCGCC G[GA]^CG[CT]C
HincII 820 GTTAAC GT[CT]^[GA]AC
HindII 820 GTTAAC GT[CT]^[GA]AC
HindIII 1232 AAGCTT A^AGCTT
HpaI 820 GTTAAC GTT^AAC
KasI 214 GGCGCC G^GCGCC
MluNI 1200 TGGCCA TGG^CCA
MroI 1089 TCCGGA T^CCGGA
MscI 1200 TGGCCA TGG^CCA
NarI 214 GGCGCC GG^CGCC
NsiI 1694 ATGCAT ATGCA^T
NspI 1602 ACATGC [GA]CATG^[CT]
NspV 587 TTCGAA TT^CGAA
PflMI 57 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1224 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1252 ATTAAT AT^TAAT
RcaI 1169 TCATGA T^CATGA
ScaI 1641 AGTACT AGT^ACT
SfoI 214 GGCGCC GGC^GCC
SfuI 587 TTCGAA TT^CGAA
SspBI 1512 TGTACA T^GTACA
SspI 1486 AATATT AAT^ATT
Van91I 57 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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Asp700I 940 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1273 GAAGGGCTTC
BanI 76 GGCACC G^G[CT][GA]CC
214 GGCGCC
BglII 615 AGATCT A^GATCT
684 AGATCT
BpmI 794 CTCCAG CTGGAG(16/14)
461 CTGGAG
BsaBI 24 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
685 GATCTCGATC
BsgI 1931 CTGCAC GTGCAG(16/14)
632 GTGCAG
BspMI 89 ACCTGC ACCTGC(4/8)
51 GCAGGT
BsrI 578 ACTGG ACTGG(1/-1)
1268 CCAGT
BstXI 1622 CCAAGGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1703 CCATCACGGTGG
EaeI 208 TGGCCG [CT]^GGCC[GA]
1200 TGGCCA
EarI 826 CTCTTC CTCTTC(1/4)
1526 GAAGAG
EcoT14I 280 CCTTGG C^C[AT][AT]GG
1622 CCAAGG
FauI 96 CCCGC CCCGC(4/6)
181 GCGGG
HgaI 196 GACGC GACGC(5/10)
123 GCGTC
Ksp632I 826 CTCTTC CTCTTC(1/4)
1526 GAAGAG
MaeII 85 ACGT A^CGT
427 ACGT
MamI 24 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
685 GATCTCGATC
MfeI 433 CAATTG C^AATTG
1480 CAATTG
MspA1I 97 CCGCTG C[AC]G^C[GT]G
133 CAGCGG
MunI 433 CAATTG C^AATTG
1480 CAATTG
SexAI 855 ACCAGGT A^CC[AT]GGT
1595 ACCAGGT
SmlI 1158 CTTGAG C^T[CT][GA]AG
1229 CTCAAG
StyI 280 CCTTGG C^C[AT][AT]GG
1622 CCAAGG
XmnI 940 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1273 GAAGGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaII BbvI BcnI
BfaI BmyI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII