################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AccI AclI AcsI AflII AflIII AgeI AhdI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsgI BsmI Bsp1407I BspDI BspHI BspLU11I BsrGI BssSI Bst1107I BstZ17I CelII ClaI CpoI DraI DraIII Eam1105I Eco47III EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII KasI MamI MfeI MluI MluNI MscI MunI NarI NcoI NdeI NotI NruI NsiI PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tsp509I Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 175 GACGTC GACGT^C Acc65I 1187 GGTACC G^GTACC AccIII 74 TCCGGA T^CCGGA Alw44I 1345 GTGCAC G^TGCAC AocI 1154 CCTCAGG CC^T[ACGT]AGG ApaLI 1345 GTGCAC G^TGCAC Asp718I 1187 GGTACC G^GTACC BbvCI 1231 GCTGAGG CCTCAGC(-5/-2) BcgI 1736 GCATATCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 503 CTAG C^TAG BsaI 492 GGTCTC GGTCTC(1/5) BseAI 74 TCCGGA T^CCGGA BsiWI 975 CGTACG C^GTACG BsmBI 17 CGTCTC CGTCTC(1/5) BspEI 74 TCCGGA T^CCGGA BspMI 1224 GCAGGT ACCTGC(4/8) BsrBI 1264 GAGCGG CCGCTC(-3/-3) BsrDI 389 GCAATG GCAATG(2/0) BssHII 1502 GCGCGC G^CGCGC BstAPI 916 GCACTCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1414 TTCGAA TT^CGAA BstEII 451 GGTGACC G^GT[ACGT]ACC BstPI 451 GGTGACC G^GT[ACGT]ACC BstYI 659 AGATCC [GA]^GATC[CT] Bsu36I 1154 CCTCAGG CC^T[ACGT]AGG DrdI 511 GACCCCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 334 CGGCCG C^GGCCG EclXI 334 CGGCCG C^GGCCG Eco52I 334 CGGCCG C^GGCCG Eco81I 1154 CCTCAGG CC^T[ACGT]AGG EcoNI 1542 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 451 GGTGACC G^GT[ACGT]ACC HpaI 1210 GTTAAC GTT^AAC KpnI 1187 GGTACC GGTAC^C KspI 904 CCGCGG CCGC^GG MaeI 503 CTAG C^TAG MaeII 176 ACGT A^CGT MflI 659 AGATCC [GA]^GATC[CT] MroI 74 TCCGGA T^CCGGA NheI 502 GCTAGC G^CTAGC NspV 1414 TTCGAA TT^CGAA PpuMI 798 GGGACCC [GA]G^G[AT]CC[CT] SacI 202 GAGCTC GAGCT^C SacII 904 CCGCGG CCGC^GG SapI 381 GAAGAGC GCTCTTC(1/4) SfuI 1414 TTCGAA TT^CGAA SphI 600 GCATGC GCATG^C TfiI 1661 GAATC G^A[AT]TC XcmI 1364 CCAGGACAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 659 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 905 CGCG CG^CG 1503 CGCG BglI 208 GCCAAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 608 GCCCAGAGGGC BsaWI 74 TCCGGA [AT]^CCGG[AT] 1253 TCCGGT BsiEI 334 CGGCCG CG[GA][CT]^CG 679 CGACCG BstUI 905 CGCG CG^CG 1503 CGCG BstXI 578 CCAGCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1012 CCAGTGTGCTGG DraII 780 GGGGCCT [GA]G^G[ACGT]CC[CT] 798 GGGACCC DsaI 904 CCGCGG C^C[GA][CT]GG 1610 CCACGG EarI 381 GAAGAG CTCTTC(1/4) 1182 GAAGAG EcoO109I 780 GGGGCCT [GA]G^G[ACGT]CC[CT] 798 GGGACCC HincII 667 GTCAAC GT[CT]^[GA]AC 1210 GTTAAC HindII 667 GTCAAC GT[CT]^[GA]AC 1210 GTTAAC Ksp632I 381 GAAGAG CTCTTC(1/4) 1182 GAAGAG MseI 1211 TTAA T^TAA 1258 TTAA MslI 262 CACCAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 927 CACACAGGTG MvnI 905 CGCG CG^CG 1503 CGCG NspI 600 GCATGC [GA]CATG^[CT] 1584 GCATGT PaeR7I 985 CTCGAG C^TCGAG 1196 CTCGAG PflMI 1044 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1670 CCAGCCAGTGG PstI 46 CTGCAG CTGCA^G 748 CTGCAG PvuII 754 CAGCTG CAG^CTG 1579 CAGCTG SfcI 46 CTGCAG C^T[GA][CT]AG 748 CTGCAG Tru9I 1211 TTAA T^TAA 1258 TTAA Van91I 1044 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1670 CCAGCCAGTGG XhoI 985 CTCGAG C^TCGAG 1196 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmaI SmlI StyI TaqI TseI Tsp45I TspRI TthHB8I XmaCI XmaI