Restriction map for pF1KB6386
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        AocI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BbeI        BbvCI       BciVI       BclI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaHI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BssHII      BssSI       BstEII      BstPI       Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NotI        NruI        NsiI        NspI        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        ScaI        SexAI       
SfaNI       SfiI        SfoI        SgrAI       SmaI        
SmlI        SpeI        SphI        SspI        StuI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

AccI          657  GTATAC               GT^[AC][GT]AC
AccII         445  CGCG                 CG^CG
AcsI          825  GAATTT               [GA]^AATT[CT]
AfaI          421  GTAC                 GT^AC
Alw44I        696  GTGCAC               G^TGCAC
AlwNI         449  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       128  AGCGCT               AGC^GCT
ApaI          437  GGGCCC               GGGCC^C
ApaLI         696  GTGCAC               G^TGCAC
ApoI          825  GAATTT               [GA]^AATT[CT]
Asp700I       822  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI          778  GGCACC               G^G[CT][GA]CC
BbrPI          40  CACGTG               CAC^GTG
BbsI          754  GAAGAC               GAAGAC(2/6)
BcgI          698  GCACTCATTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI         754  GAAGAC               GAAGAC(2/6)
BsaI          408  GGTCTC               GGTCTC(1/5)
BsaWI         428  ACCGGA               [AT]^CCGG[AT]
Bsp1407I      420  TGTACA               T^GTACA
BspMI         457  GCAGGT               ACCTGC(4/8)
BsrDI         491  GCAATG               GCAATG(2/0)
BsrFI         137  ACCGGC               [GA]^CCGG[CT]
BsrGI         420  TGTACA               T^GTACA
Bst1107I      657  GTATAC               GTA^TAC
BstBI         706  TTCGAA               TT^CGAA
BstF5I        692  GGATG                GGATG(2/0)
BstUI         445  CGCG                 CG^CG
BstXI         155  CCAACCCCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       657  GTATAC               GTA^TAC
Cfr10I        137  ACCGGC               [GA]^CCGG[CT]
DsaI          161  CCATGG               C^C[GA][CT]GG
EaeI           98  TGGCCG               [CT]^GGCC[GA]
Eco47III      128  AGCGCT               AGC^GCT
EcoT14I       161  CCATGG               C^C[AT][AT]GG
FokI          692  GGATG                GGATG(9/13)
HaeII         128  AGCGCT               [GA]GCGC^[CT]
HgaI            7  GCGTC                GACGC(5/10)
MseI           13  TTAA                 T^TAA
MvnI          445  CGCG                 CG^CG
NcoI          161  CCATGG               C^CATGG
NheI          615  GCTAGC               G^CTAGC
NspV          706  TTCGAA               TT^CGAA
PleI           87  GACTC                GAGTC(4/5)
PmaCI          40  CACGTG               CAC^GTG
PmlI           40  CACGTG               CAC^GTG
PpuMI         743  AGGACCT              [GA]G^G[AT]CC[CT]
PstI          455  CTGCAG               CTGCA^G
PvuII         532  CAGCTG               CAG^CTG
RsaI          421  GTAC                 GT^AC
SacI          293  GAGCTC               GAGCT^C
SapI          709  GAAGAGC              GCTCTTC(1/4)
SbfI          454  CCTGCAGG             CCTGCA^GG
SfcI          455  CTGCAG               C^T[GA][CT]AG
SfuI          706  TTCGAA               TT^CGAA
SnaBI         809  TACGTA               TAC^GTA
Sse8387I      454  CCTGCAGG             CCTGCA^GG
SspBI         420  TGTACA               T^GTACA
StyI          161  CCATGG               C^C[AT][AT]GG
TaqI          707  TCGA                 T^CGA
Tru9I          13  TTAA                 T^TAA
Tsp509I       826  AATT                 ^AATT
TthHB8I       707  TCGA                 T^CGA
XmnI          822  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AvaII         168  GGACC                G^G[AT]CC
              744  GGACC               
BanII         293  GAGCTC               G[GA]GC[CT]^C
              437  GGGCCC              
BcnI           62  CCCGG                CC^[GC]GG
              399  CCGGG               
BfaI          616  CTAG                 C^TAG
              775  CTAG                
BsaJI          61  CCCCGG               C^C[ACGT][ACGT]GG
              161  CCATGG              
BseRI         199  GAGGAG               GAGGAG(10/8)
              790  GAGGAG              
BsmFI         768  GGGAC                GGGAC(10/14)
              605  GTCCC               
BstAPI        448  GCAGCTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              619  GCAGAATATGC         
BstNI         867  CCAGG                CC^[AT]GG
              895  CCTGG               
BstYI         395  AGATCC               [GA]^GATC[CT]
              855  AGATCC              
EarI          553  CTCTTC               CTCTTC(1/4)
              709  GAAGAG              
EcoRII        867  CCAGG                ^CC[AT]GG
              895  CCTGG               
HphI          402  GGTGA                GGTGA(8/7)
              358  TCACC               
Ksp632I       553  CTCTTC               CTCTTC(1/4)
              709  GAAGAG              
MaeI          616  CTAG                 C^TAG
              775  CTAG                
MflI          395  AGATCC               [GA]^GATC[CT]
              855  AGATCC              
MspA1I        361  CCGCTG               C[AC]G^C[GT]G
              532  CAGCTG              
MvaI          867  CCAGG                CC^[AT]GG
              895  CCTGG               
NciI           62  CCCGG                CC^[GC]GG
              399  CCGGG               
TfiI          432  GATTC                G^A[AT]TC
              596  GAATC               
TspRI         719  CACTG                CA[GC]TG[ACGT][ACGT]^
              872  CAGTG               
XhoII         395  AGATCC               [GA]^GATC[CT]
              855  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AciI        AluI        AlwI        AspHI       BbvI        
BmyI        BpmI        BsaAI       BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EcoO109I    Fnu4HI      HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       ItaI        
MaeII       MaeIII      MboI        MboII       MnlI        
MspI        MwoI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       TseI        Tsp45I      
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