################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI AocI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbvCI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BssHII BssSI BstEII BstPI Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MslI MunI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfaNI SfiI SfoI SgrAI SmaI SmlI SpeI SphI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AccI 657 GTATAC GT^[AC][GT]AC AccII 445 CGCG CG^CG AcsI 825 GAATTT [GA]^AATT[CT] AfaI 421 GTAC GT^AC Alw44I 696 GTGCAC G^TGCAC AlwNI 449 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 128 AGCGCT AGC^GCT ApaI 437 GGGCCC GGGCC^C ApaLI 696 GTGCAC G^TGCAC ApoI 825 GAATTT [GA]^AATT[CT] Asp700I 822 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 778 GGCACC G^G[CT][GA]CC BbrPI 40 CACGTG CAC^GTG BbsI 754 GAAGAC GAAGAC(2/6) BcgI 698 GCACTCATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 754 GAAGAC GAAGAC(2/6) BsaI 408 GGTCTC GGTCTC(1/5) BsaWI 428 ACCGGA [AT]^CCGG[AT] Bsp1407I 420 TGTACA T^GTACA BspMI 457 GCAGGT ACCTGC(4/8) BsrDI 491 GCAATG GCAATG(2/0) BsrFI 137 ACCGGC [GA]^CCGG[CT] BsrGI 420 TGTACA T^GTACA Bst1107I 657 GTATAC GTA^TAC BstBI 706 TTCGAA TT^CGAA BstF5I 692 GGATG GGATG(2/0) BstUI 445 CGCG CG^CG BstXI 155 CCAACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 657 GTATAC GTA^TAC Cfr10I 137 ACCGGC [GA]^CCGG[CT] DsaI 161 CCATGG C^C[GA][CT]GG EaeI 98 TGGCCG [CT]^GGCC[GA] Eco47III 128 AGCGCT AGC^GCT EcoT14I 161 CCATGG C^C[AT][AT]GG FokI 692 GGATG GGATG(9/13) HaeII 128 AGCGCT [GA]GCGC^[CT] HgaI 7 GCGTC GACGC(5/10) MseI 13 TTAA T^TAA MvnI 445 CGCG CG^CG NcoI 161 CCATGG C^CATGG NheI 615 GCTAGC G^CTAGC NspV 706 TTCGAA TT^CGAA PleI 87 GACTC GAGTC(4/5) PmaCI 40 CACGTG CAC^GTG PmlI 40 CACGTG CAC^GTG PpuMI 743 AGGACCT [GA]G^G[AT]CC[CT] PstI 455 CTGCAG CTGCA^G PvuII 532 CAGCTG CAG^CTG RsaI 421 GTAC GT^AC SacI 293 GAGCTC GAGCT^C SapI 709 GAAGAGC GCTCTTC(1/4) SbfI 454 CCTGCAGG CCTGCA^GG SfcI 455 CTGCAG C^T[GA][CT]AG SfuI 706 TTCGAA TT^CGAA SnaBI 809 TACGTA TAC^GTA Sse8387I 454 CCTGCAGG CCTGCA^GG SspBI 420 TGTACA T^GTACA StyI 161 CCATGG C^C[AT][AT]GG TaqI 707 TCGA T^CGA Tru9I 13 TTAA T^TAA Tsp509I 826 AATT ^AATT TthHB8I 707 TCGA T^CGA XmnI 822 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AvaII 168 GGACC G^G[AT]CC 744 GGACC BanII 293 GAGCTC G[GA]GC[CT]^C 437 GGGCCC BcnI 62 CCCGG CC^[GC]GG 399 CCGGG BfaI 616 CTAG C^TAG 775 CTAG BsaJI 61 CCCCGG C^C[ACGT][ACGT]GG 161 CCATGG BseRI 199 GAGGAG GAGGAG(10/8) 790 GAGGAG BsmFI 768 GGGAC GGGAC(10/14) 605 GTCCC BstAPI 448 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 619 GCAGAATATGC BstNI 867 CCAGG CC^[AT]GG 895 CCTGG BstYI 395 AGATCC [GA]^GATC[CT] 855 AGATCC EarI 553 CTCTTC CTCTTC(1/4) 709 GAAGAG EcoRII 867 CCAGG ^CC[AT]GG 895 CCTGG HphI 402 GGTGA GGTGA(8/7) 358 TCACC Ksp632I 553 CTCTTC CTCTTC(1/4) 709 GAAGAG MaeI 616 CTAG C^TAG 775 CTAG MflI 395 AGATCC [GA]^GATC[CT] 855 AGATCC MspA1I 361 CCGCTG C[AC]G^C[GT]G 532 CAGCTG MvaI 867 CCAGG CC^[AT]GG 895 CCTGG NciI 62 CCCGG CC^[GC]GG 399 CCGGG TfiI 432 GATTC G^A[AT]TC 596 GAATC TspRI 719 CACTG CA[GC]TG[ACGT][ACGT]^ 872 CAGTG XhoII 395 AGATCC [GA]^GATC[CT] 855 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AluI AlwI AspHI BbvI BmyI BpmI BsaAI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeII MaeIII MboI MboII MnlI MspI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI Tsp45I