Restriction map for
pF1KB7012
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# NO CUT ENZYMES ( 68 enzymes) #
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AatII AclI AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspI AviII AvrII BfrI
BglII BlnI BsiEI BsiWI BsmI
BspDI BspLU11I BsrBI BssHII Bst1107I
BstBI BstZ17I ClaI DraI EagI
EclXI Eco47III Eco52I EcoRV EcoT22I
FseI FspI HindIII HpaI MfeI
MluI MunI NdeI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PvuI
SalI SfiI SfuI SgrAI SnaBI
SpeI SspI StuI SwaI Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 2108 TCCGGA T^CCGGA
AflIII 1754 ACACGT A^C[GA][CT]GT
AhdI 1780 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2141 CCTCAGG CC^T[ACGT]AGG
AspEI 1780 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 2293 GGCGCC GGCGC^C
BbrPI 1755 CACGTG CAC^GTG
BbvCI 2422 GCTGAGG CCTCAGC(-5/-2)
BciVI 2525 GTATCC GTATCC(6/5)
BclI 1418 TGATCA T^GATCA
BsaAI 1755 CACGTG [CT]AC^GT[GA]
BseAI 2108 TCCGGA T^CCGGA
BsmBI 2222 GAGACG CGTCTC(1/5)
Bsp1407I 575 TGTACA T^GTACA
BspEI 2108 TCCGGA T^CCGGA
BsrGI 575 TGTACA T^GTACA
BssSI 2410 CTCGTG CACGAG(-5/-1)
BstEII 768 GGTCACC G^GT[ACGT]ACC
BstPI 768 GGTCACC G^GT[ACGT]ACC
Bsu36I 2141 CCTCAGG CC^T[ACGT]AGG
CpoI 2582 CGGACCG CG^G[AT]CCG
Eam1105I 1780 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2141 CCTCAGG CC^T[ACGT]AGG
EcoNI 2246 CCTTCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 768 GGTCACC G^GT[ACGT]ACC
EcoRI 400 GAATTC G^AATTC
FbaI 1418 TGATCA T^GATCA
KasI 2293 GGCGCC G^GCGCC
MroI 2108 TCCGGA T^CCGGA
MseI 2789 TTAA T^TAA
NaeI 1051 GCCGGC GCC^GGC
NarI 2293 GGCGCC GG^CGCC
NgoMIV 1051 GCCGGC G^CCGGC
NheI 1227 GCTAGC G^CTAGC
PmaCI 1755 CACGTG CAC^GTG
PmlI 1755 CACGTG CAC^GTG
PshAI 538 GACGGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 2582 CGGACCG CG^G[AT]CCG
SapI 2428 GAAGAGC GCTCTTC(1/4)
SbfI 1945 CCTGCAGG CCTGCA^GG
ScaI 2306 AGTACT AGT^ACT
SexAI 281 ACCTGGT A^CC[AT]GGT
SfoI 2293 GGCGCC GGC^GCC
SphI 178 GCATGC GCATG^C
Sse8387I 1945 CCTGCAGG CCTGCA^GG
SspBI 575 TGTACA T^GTACA
Tru9I 2789 TTAA T^TAA
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# TWO CUT ENZYMES ( 30 enzymes) #
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Acc65I 229 GGTACC G^GTACC
1204 GGTACC
AccI 322 GTCTAC GT^[AC][GT]AC
2314 GTCTAC
AcsI 400 GAATTC [GA]^AATT[CT]
2948 AAATTC
ApoI 400 GAATTC [GA]^AATT[CT]
2948 AAATTC
Asp718I 229 GGTACC G^GTACC
1204 GGTACC
BalI 623 TGGCCA TGG^CCA
2931 TGGCCA
BamHI 88 GGATCC G^GATCC
2978 GGATCC
BcgI 1989 CGAGGGCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2122 GCAGCCCCCTCG
BsaWI 1679 ACCGGA [AT]^CCGG[AT]
2108 TCCGGA
BspHI 290 TCATGA T^CATGA
435 TCATGA
BsrDI 579 CATTGC GCAATG(2/0)
1994 GCAATG
BstAPI 165 GCAGGTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
307 GCACAGGATGC
DrdI 2193 GACGCCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2797 GACCCTGTGGTC
EaeI 623 TGGCCA [CT]^GGCC[GA]
2931 TGGCCA
EarI 63 CTCTTC CTCTTC(1/4)
2428 GAAGAG
HaeII 1219 AGCGCC [GA]GCGC^[CT]
2293 GGCGCC
HincII 1378 GTCAAC GT[CT]^[GA]AC
2185 GTCAAC
HindII 1378 GTCAAC GT[CT]^[GA]AC
2185 GTCAAC
KpnI 229 GGTACC GGTAC^C
1204 GGTACC
Ksp632I 63 CTCTTC CTCTTC(1/4)
2428 GAAGAG
MaeII 1756 ACGT A^CGT
2489 ACGT
MluNI 623 TGGCCA TGG^CCA
2931 TGGCCA
MscI 623 TGGCCA TGG^CCA
2931 TGGCCA
NcoI 1610 CCATGG C^CATGG
1740 CCATGG
NspI 178 GCATGC [GA]CATG^[CT]
1172 GCATGT
RcaI 290 TCATGA T^CATGA
435 TCATGA
SacI 2176 GAGCTC GAGCT^C
3115 GAGCTC
SmlI 1545 CTCAAG C^T[CT][GA]AG
2769 CTCAAG
TfiI 1263 GAATC G^A[AT]TC
1502 GAATC
Tsp509I 401 AATT ^AATT
2949 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KspI MaeI MaeIII MamI
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI