################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccI AccIII AflII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AviII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglII BlpI Bpu1102I BsaAI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraII DrdI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HhaI HinP1I HincII HindII HpaI KasI KpnI KspI MfeI MflI MluI MluNI MroI MscI MspA1I MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccII 1632 CGCG CG^CG AclI 983 AACGTT AA^CGTT AcsI 249 AAATTC [GA]^AATT[CT] AcyI 381 GACGCC G[GA]^CG[CT]C AfaI 576 GTAC GT^AC AflIII 1406 ACATGT A^C[GA][CT]GT AhdI 331 GACCTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 249 AAATTC [GA]^AATT[CT] AspEI 331 GACCTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 87 GGTCC G^G[AT]CC AvrII 421 CCTAGG C^CTAGG BanII 27 GAGCCC G[GA]GC[CT]^C BbvI 806 GCTGC GCAGC(8/12) BclI 1146 TGATCA T^GATCA BglI 1543 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 421 CCTAGG C^CTAGG BpmI 1043 CTGGAG CTGGAG(16/14) BsaBI 898 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 381 GACGCC G[GA]^CG[CT]C BsgI 1410 GTGCAG GTGCAG(16/14) BspHI 57 TCATGA T^CATGA BspLU11I 1406 ACATGT A^CATGT BsrFI 1127 ACCGGC [GA]^CCGG[CT] BstUI 1632 CGCG CG^CG BstXI 1035 CCATCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 1629 GCACGC GC[ACGT]^[ACGT]GC Cfr10I 1127 ACCGGC [GA]^CCGG[CT] DraIII 1277 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1281 CCGTGG C^C[GA][CT]GG Eam1105I 331 GACCTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT14I 421 CCTAGG C^C[AT][AT]GG FbaI 1146 TGATCA T^GATCA HapII 1128 CCGG C^CGG HgaI 381 GACGC GACGC(5/10) Hin1I 381 GACGCC G[GA]^CG[CT]C HpaII 1128 CCGG C^CGG MaeII 984 ACGT A^CGT MamI 898 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspI 1128 CCGG C^CGG MvnI 1632 CGCG CG^CG MwoI 1543 GCCAAAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 184 GGAACC GG[ACGT]^[ACGT]CC PleI 1420 GAGTC GAGTC(4/5) Psp1406I 983 AACGTT AA^CGTT PstI 716 CTGCAG CTGCA^G RcaI 57 TCATGA T^CATGA RsaI 576 GTAC GT^AC SfaNI 1623 GCATC GCATC(5/9) SfcI 716 CTGCAG C^T[GA][CT]AG StyI 421 CCTAGG C^C[AT][AT]GG TseI 806 GCTGC G^C[AT]GC XbaI 195 TCTAGA T^CTAGA XcmI 1538 CCAGTGCCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 54 GGATC GGATC(4/5) 1062 GGATC AlwNI 938 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG 1460 CAGTCCCTG BmyI 27 GAGCCC G[AGT]GC[ACT]^C 1610 GTGCCC BsaI 1428 GAGACC GGTCTC(1/5) 1591 GAGACC Bsp1286I 27 GAGCCC G[AGT]GC[ACT]^C 1610 GTGCCC BssKI 1042 CCTGG ^CC[ACGT]GG 1500 CCTGG BstNI 1042 CCTGG CC^[AT]GG 1500 CCTGG Cfr13I 87 GGTCC G^G[ACGT]CC 1635 GGCCC EcoRII 1042 CCTGG ^CC[AT]GG 1500 CCTGG Fnu4HI 806 GCTGC GC^[ACGT]GC 1633 GCGGC HindIII 864 AAGCTT A^AGCTT 1085 AAGCTT ItaI 806 GCTGC GC^[ACGT]GC 1633 GCGGC MslI 91 CATGACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1230 CACAGATGTG MvaI 1042 CCTGG CC^[AT]GG 1500 CCTGG Sau96I 87 GGTCC G^G[ACGT]CC 1635 GGCCC ScrFI 1042 CCTGG CC^[ACGT]GG 1500 CCTGG SmlI 763 CTCAAG C^T[CT][GA]AG 824 CTCAAG StuI 424 AGGCCT AGG^CCT 818 AGGCCT TaqI 25 TCGA T^CGA 794 TCGA Tsp45I 391 GTGAC ^GT[GC]AC 1184 GTGAC TthHB8I 25 TCGA T^CGA 794 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AluI BbsI BfaI BpuAI BsaJI BsiYI BslI BsmAI BsmFI BsmI BsrDI BsrI BstF5I DdeI DpnII EarI FokI HaeIII HinfI HphI Ksp632I MaeI MaeIII MboI MboII MnlI MseI NdeII NlaIII NspI Sau3AI TfiI Tru9I Tsp509I TspRI