Restriction map for
pF1KB8217
##################################
# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI Alw44I AlwI AocI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BpuAI BsaBI BseRI BsgI BsiEI
BsiWI BsmBI BsmI BspDI BspLU11I
BsrBI BsrDI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I ClaI CpoI DpnII
DraI DraIII DrdI DsaI EagI
EarI EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HindIII HinfI HpaI
HphI KasI KpnI Ksp632I KspI
MaeIII MamI MboI MfeI MflI
MluI MseI MunI NarI NcoI
NdeI NdeII NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacII SapI Sau3AI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI TfiI
Tru9I Tsp45I Tsp509I Tth111I XbaI
XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 847 GTCGAC GT^[AC][GT]AC
AccIII 584 TCCGGA T^CCGGA
AcyI 772 GGCGTC G[GA]^CG[CT]C
AflIII 455 ACGTGT A^C[GA][CT]GT
AhdI 558 GACCATGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 161 AGCGCT AGC^GCT
AspEI 558 GACCATGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 659 TGGCCA TGG^CCA
BglI 218 GCCCAAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 859 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 859 GCTGAGC GC^T[ACGT]AGC
BsaAI 454 TACGTG [CT]AC^GT[GA]
BsaHI 772 GGCGTC G[GA]^CG[CT]C
BsaWI 584 TCCGGA [AT]^CCGG[AT]
BseAI 584 TCCGGA T^CCGGA
Bsp1407I 458 TGTACA T^GTACA
BspEI 584 TCCGGA T^CCGGA
BspHI 434 TCATGA T^CATGA
BspMI 105 ACCTGC ACCTGC(4/8)
BsrGI 458 TGTACA T^GTACA
BssHII 7 GCGCGC G^CGCGC
CelII 859 GCTGAGC GC^T[ACGT]AGC
DdeI 860 CTGAG C^T[ACGT]AG
Eam1105I 558 GACCATGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 161 AGCGCT AGC^GCT
EcoNI 690 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 161 AGCGCT [GA]GCGC^[CT]
Hin1I 772 GGCGTC G[GA]^CG[CT]C
HincII 847 GTCGAC GT[CT]^[GA]AC
HindII 847 GTCGAC GT[CT]^[GA]AC
MaeII 455 ACGT A^CGT
MboII 254 TCTTC GAAGA(8/7)
MluNI 659 TGGCCA TGG^CCA
MroI 584 TCCGGA T^CCGGA
MscI 659 TGGCCA TGG^CCA
MslI 429 CATGTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 12 CAGCGG C[AC]G^C[GT]G
PflMI 651 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 434 TCATGA T^CATGA
SacI 622 GAGCTC GAGCT^C
SalI 847 GTCGAC G^TCGAC
SspBI 458 TGTACA T^GTACA
Van91I 651 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 390 CCACTCAAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwNI 652 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
828 CAGCCCCTG
AspHI 530 GAGCAC G[AT]GC[AT]^C
622 GAGCTC
AvaI 281 CCCGGG C^[CT]CG[GA]G
764 CCCGGG
BfaI 62 CTAG C^TAG
304 CTAG
BsaI 352 GAGACC GGTCTC(1/5)
556 GAGACC
BsiHKAI 530 GAGCAC G[AT]GC[AT]^C
622 GAGCTC
BsmAI 352 GAGAC GTCTC(1/5)
556 GAGAC
BsoBI 281 CCCGGG C^[CT]CG[GA]G
764 CCCGGG
MaeI 62 CTAG C^TAG
304 CTAG
NspI 428 GCATGT [GA]CATG^[CT]
473 GCATGT
PstI 107 CTGCAG CTGCA^G
627 CTGCAG
SmaI 281 CCCGGG CCC^GGG
764 CCCGGG
TaqI 257 TCGA T^CGA
848 TCGA
TthHB8I 257 TCGA T^CGA
848 TCGA
XmaCI 281 CCCGGG C^CCGGG
764 CCCGGG
XmaI 281 CCCGGG C^CCGGG
764 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AfaI AluI ApaI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HpaII
ItaI MnlI MspI MvaI MvnI
MwoI NaeI NciI NgoMIV NlaIII
NlaIV PpuMI RsaI Sau96I ScrFI
SfaNI SfcI SmlI TseI TspRI