################################## # NO CUT ENZYMES ( 186 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BsrI BssHII Bst1107I BstBI BstEII BstF5I BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MspA1I MunI NarI NcoI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I TspRI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 433 GTCTAC GT^[AC][GT]AC AccII 14 CGCG CG^CG AcsI 139 GAATTC [GA]^AATT[CT] AfaI 353 GTAC GT^AC ApoI 139 GAATTC [GA]^AATT[CT] AvaII 108 GGACC G^G[AT]CC BcnI 158 CCCGG CC^[GC]GG BpmI 166 CTGGAG CTGGAG(16/14) BsaWI 149 ACCGGA [AT]^CCGG[AT] BsgI 54 GTGCAG GTGCAG(16/14) BsiEI 10 CGGTCG CG[GA][CT]^CG BspMI 56 GCAGGT ACCTGC(4/8) BsrBI 193 GAGCGG CCGCTC(-3/-3) BsrFI 87 GCCGGC [GA]^CCGG[CT] BssSI 320 CTCGTG CACGAG(-5/-1) BstAPI 234 GCACAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 14 CGCG CG^CG CfoI 4 GCGC GCG^C Cfr10I 87 GCCGGC [GA]^CCGG[CT] Cfr13I 108 GGACC G^G[ACGT]CC DsaI 448 CCGTGG C^C[GA][CT]GG EcoRI 139 GAATTC G^AATTC HaeII 3 GGCGCT [GA]GCGC^[CT] HaeIII 312 GGCC GG^CC HhaI 4 GCGC GCG^C HinP1I 4 GCGC G^CGC HincII 72 GTCAAC GT[CT]^[GA]AC HindII 72 GTCAAC GT[CT]^[GA]AC HphI 143 TCACC GGTGA(8/7) MseI 335 TTAA T^TAA MvnI 14 CGCG CG^CG NaeI 87 GCCGGC GCC^GGC NciI 158 CCCGG CC^[GC]GG NgoMIV 87 GCCGGC G^CCGGC RsaI 353 GTAC GT^AC Sau96I 108 GGACC G^G[ACGT]CC SfcI 179 CTGTAG C^T[GA][CT]AG TaqI 469 TCGA T^CGA Tru9I 335 TTAA T^TAA Tsp509I 140 AATT ^AATT TthHB8I 469 TCGA T^CGA XcmI 356 CCAGAAATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### BsiYI 159 CCGGAACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 447 CCCGTGGGAGG BslI 159 CCGGAACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 447 CCCGTGGGAGG BstXI 356 CCAGAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 442 CCAACCCCGTGG FauI 195 GCGGG CCCGC(4/6) 415 GCGGG MslI 286 CATCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 398 CACAGAGATG PleI 255 GACTC GAGTC(4/5) 466 GACTC TfiI 419 GAATC G^A[AT]TC 530 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AciI AluI BbvI BsaJI BssKI BstNI Cac8I DdeI EcoRII Fnu4HI HapII HinfI HpaII ItaI MnlI MspI MvaI MwoI NlaIII NlaIV ScrFI SfaNI TseI