Restriction map for
pF1KE0533
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BanI BbeI BbrPI
BbsI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BssHII BssKI BssSI Bst1107I BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoRV EcoT22I FbaI FokI
FseI FspI HapII Hin1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MaeII MaeIII MamI
MfeI MluI MluNI MroI MscI
MspA1I MspI MunI MvaI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NotI NruI NsiI NspI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI ScrFI SexAI SfaNI
SfcI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tsp45I TspRI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AocI 304 CCTCAGG CC^T[ACGT]AGG
Aor51HI 197 AGCGCT AGC^GCT
AvaII 362 GGTCC G^G[AT]CC
BamHI 492 GGATCC G^GATCC
BbvCI 475 GCTGAGG CCTCAGC(-5/-2)
BpmI 439 CTGGAG CTGGAG(16/14)
BsaJI 587 CCAAGG C^C[ACGT][ACGT]GG
BsmFI 398 GGGAC GGGAC(10/14)
BspMI 569 GCAGGT ACCTGC(4/8)
BsrBI 298 GAGCGG CCGCTC(-3/-3)
BstBI 149 TTCGAA TT^CGAA
Bsu36I 304 CCTCAGG CC^T[ACGT]AGG
EarI 146 CTCTTC CTCTTC(1/4)
Eco47III 197 AGCGCT AGC^GCT
Eco81I 304 CCTCAGG CC^T[ACGT]AGG
EcoT14I 587 CCAAGG C^C[AT][AT]GG
HaeII 197 AGCGCT [GA]GCGC^[CT]
Ksp632I 146 CTCTTC CTCTTC(1/4)
MseI 611 TTAA T^TAA
NheI 30 GCTAGC G^CTAGC
NlaIV 492 GGATCC GG[ACGT]^[ACGT]CC
NspV 149 TTCGAA TT^CGAA
PleI 87 GACTC GAGTC(4/5)
SfuI 149 TTCGAA TT^CGAA
SmlI 236 CTCAAG C^T[CT][GA]AG
StyI 587 CCAAGG C^C[AT][AT]GG
TaqI 150 TCGA T^CGA
TfiI 457 GAATC G^A[AT]TC
Tru9I 611 TTAA T^TAA
Tsp509I 246 AATT ^AATT
TthHB8I 150 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Asp700I 121 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
142 GAACCTCTTC
AspHI 313 GAGCTC G[AT]GC[AT]^C
341 GAGCTC
BanII 313 GAGCTC G[GA]GC[CT]^C
341 GAGCTC
BmyI 313 GAGCTC G[AGT]GC[ACT]^C
341 GAGCTC
BseRI 325 GAGGAG GAGGAG(10/8)
336 GAGGAG
BsiHKAI 313 GAGCTC G[AT]GC[AT]^C
341 GAGCTC
BsmAI 85 GAGAC GTCTC(1/5)
328 GAGAC
Bsp1286I 313 GAGCTC G[AGT]GC[ACT]^C
341 GAGCTC
Bsp1407I 368 TGTACA T^GTACA
593 TGTACA
BsrGI 368 TGTACA T^GTACA
593 TGTACA
BsrI 222 ACTGG ACTGG(1/-1)
453 ACTGG
CfoI 39 GCGC GCG^C
198 GCGC
Cfr13I 362 GGTCC G^G[ACGT]CC
523 GGGCC
FauI 50 GCGGG CCCGC(4/6)
489 GCGGG
HgaI 72 GACGC GACGC(5/10)
100 GCGTC
HhaI 39 GCGC GCG^C
198 GCGC
HinP1I 39 GCGC G^CGC
198 GCGC
HinfI 87 GACTC G^A[ACGT]TC
457 GAATC
NlaIII 132 CATG CATG^
472 CATG
SacI 313 GAGCTC GAGCT^C
341 GAGCTC
Sau96I 362 GGTCC G^G[ACGT]CC
523 GGGCC
SspBI 368 TGTACA T^GTACA
593 TGTACA
XmnI 121 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
142 GAACCTCTTC
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BfaI BsiYI BslI BstYI Cac8I
DdeI DpnII Fnu4HI HaeIII HphI
ItaI MaeI MboI MboII MflI
MnlI MslI MwoI NdeII RsaI
Sau3AI TseI XhoII