################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AhdI Alw44I ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI NarI NcoI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SmlI SnaBI SpeI Sse8387I SspBI SspI Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AfaI 90 GTAC GT^AC AgeI 985 ACCGGT A^CCGGT AocI 279 CCTGAGG CC^T[ACGT]AGG AspHI 285 GAGCAC G[AT]GC[AT]^C AvaI 1550 CCCGGG C^[CT]CG[GA]G BbsI 1170 GAAGAC GAAGAC(2/6) BbvCI 1102 CCTCAGC CCTCAGC(-5/-2) BfaI 1217 CTAG C^TAG BglI 1519 GCCTGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1018 GCTGAGC GC^T[ACGT]AGC BpmI 550 CTGGAG CTGGAG(16/14) Bpu1102I 1018 GCTGAGC GC^T[ACGT]AGC BpuAI 1170 GAAGAC GAAGAC(2/6) BsaBI 229 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 985 ACCGGT [AT]^CCGG[AT] BseRI 282 GAGGAG GAGGAG(10/8) BsgI 1156 CTGCAC GTGCAG(16/14) BsiHKAI 285 GAGCAC G[AT]GC[AT]^C BsmAI 1038 GTCTC GTCTC(1/5) BsoBI 1550 CCCGGG C^[CT]CG[GA]G BspMI 311 GCAGGT ACCTGC(4/8) BsrDI 1031 CATTGC GCAATG(2/0) BsrI 929 CCAGT ACTGG(1/-1) BstEII 1321 GGTGACC G^GT[ACGT]ACC BstPI 1321 GGTGACC G^GT[ACGT]ACC BstXI 162 CCATTTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 143 AGATCC [GA]^GATC[CT] Bsu36I 279 CCTGAGG CC^T[ACGT]AGG CelII 1018 GCTGAGC GC^T[ACGT]AGC DraI 25 TTTAAA TTT^AAA DraII 1280 AGGTCCT [GA]G^G[ACGT]CC[CT] EarI 1150 CTCTTC CTCTTC(1/4) Eco81I 279 CCTGAGG CC^T[ACGT]AGG EcoNI 756 CCTTTGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1280 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoO65I 1321 GGTGACC G^GT[ACGT]ACC HincII 751 GTTGAC GT[CT]^[GA]AC HindII 751 GTTGAC GT[CT]^[GA]AC HindIII 1294 AAGCTT A^AGCTT Ksp632I 1150 CTCTTC CTCTTC(1/4) MaeI 1217 CTAG C^TAG MamI 229 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 143 AGATCC [GA]^GATC[CT] MspA1I 547 CAGCTG C[AC]G^C[GT]G NaeI 1115 GCCGGC GCC^GGC NdeI 827 CATATG CA^TATG NgoMIV 1115 GCCGGC G^CCGGC NlaIV 908 GGAGCC GG[ACGT]^[ACGT]CC PinAI 985 ACCGGT A^CCGGT PpuMI 1280 AGGTCCT [GA]G^G[AT]CC[CT] PstI 309 CTGCAG CTGCA^G PvuII 547 CAGCTG CAG^CTG RsaI 90 GTAC GT^AC SapI 1149 GCTCTTC GCTCTTC(1/4) SfaNI 94 GATGC GCATC(5/9) SgrAI 984 CACCGGTG C[GA]^CCGG[CT]G SmaI 1550 CCCGGG CCC^GGG SphI 1139 GCATGC GCATG^C SwaI 24 ATTTAAAT ATTT^AAAT TaqI 59 TCGA T^CGA TthHB8I 59 TCGA T^CGA XhoII 143 AGATCC [GA]^GATC[CT] XmaCI 1550 CCCGGG C^CCGGG XmaI 1550 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwI 144 GATCC GGATC(4/5) 159 GATCC AlwNI 180 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1036 CAGTCTCTG Aor51HI 305 AGCGCT AGC^GCT 1093 AGCGCT AspI 328 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 855 GACTTCGTC AvaII 1049 GGTCC G^G[AT]CC 1281 GGTCC BcnI 1550 CCCGG CC^[GC]GG 1551 CCGGG BmyI 285 GAGCAC G[AGT]GC[ACT]^C 1355 GGGCAC Bsp1286I 285 GAGCAC G[AGT]GC[ACT]^C 1355 GGGCAC BsrFI 985 ACCGGT [GA]^CCGG[CT] 1115 GCCGGC Cfr10I 985 ACCGGT [GA]^CCGG[CT] 1115 GCCGGC Cfr13I 1049 GGTCC G^G[ACGT]CC 1281 GGTCC Eco47III 305 AGCGCT AGC^GCT 1093 AGCGCT EcoT14I 74 CCAAGG C^C[AT][AT]GG 1398 CCTTGG HaeIII 343 GGCC GG^CC 683 GGCC MaeII 698 ACGT A^CGT 722 ACGT MslI 163 CATTTTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 717 CATGAACGTG NciI 1550 CCCGG CC^[GC]GG 1551 CCGGG NspI 708 ACATGC [GA]CATG^[CT] 1139 GCATGC PflFI 328 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 855 GACTTCGTC Sau96I 1049 GGTCC G^G[ACGT]CC 1281 GGTCC StuI 342 AGGCCT AGG^CCT 682 AGGCCT StyI 74 CCAAGG C^C[AT][AT]GG 1398 CCTTGG TspRI 351 CACTG CA[GC]TG[ACGT][ACGT]^ 1062 CAGTG Tth111I 328 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 855 GACTTCGTC ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AccII AciI AcsI AluI ApoI BbvI BsaJI BsiYI BslI BssKI BstF5I BstNI BstUI Cac8I CfoI DdeI DpnII EcoRII Fnu4HI FokI HaeII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MseI MspI MvaI MvnI MwoI NdeII NlaIII PleI Sau3AI ScrFI SfcI TfiI Tru9I TseI Tsp45I Tsp509I