################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DpnII DraI DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MaeII MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NdeI NdeII NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 14 enzymes) # ################################### AvaII 407 GGACC G^G[AT]CC BsmBI 619 GAGACG CGTCTC(1/5) BspMI 624 GCAGGT ACCTGC(4/8) BsrBI 322 CCGCTC CCGCTC(-3/-3) BsrI 267 ACTGG ACTGG(1/-1) BstF5I 416 GGATG GGATG(2/0) DraIII 14 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 309 CTCTTC CTCTTC(1/4) FokI 416 GGATG GGATG(9/13) Ksp632I 309 CTCTTC CTCTTC(1/4) NcoI 107 CCATGG C^CATGG NspI 875 GCATGT [GA]CATG^[CT] PpuMI 406 AGGACCC [GA]G^G[AT]CC[CT] SmlI 352 CTCAAG C^T[CT][GA]AG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AfaI 567 GTAC GT^AC 879 GTAC AvaI 391 CCCGAG C^[CT]CG[GA]G 400 CCCGAG BbeI 222 GGCGCC GGCGC^C 837 GGCGCC BglI 85 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 444 GCCGGCCAGGC BlpI 456 GCTGAGC GC^T[ACGT]AGC 606 GCTCAGC Bpu1102I 456 GCTGAGC GC^T[ACGT]AGC 606 GCTCAGC BseRI 349 CTCCTC GAGGAG(10/8) 121 GAGGAG BsgI 471 CTGCAC GTGCAG(16/14) 238 GTGCAG BsiEI 319 CGGCCG CG[GA][CT]^CG 773 CGGCCG BsmAI 619 GAGAC GTCTC(1/5) 666 GAGAC BsoBI 391 CCCGAG C^[CT]CG[GA]G 400 CCCGAG BssHII 585 GCGCGC G^CGCGC 819 GCGCGC CelII 456 GCTGAGC GC^T[ACGT]AGC 606 GCTCAGC DraII 74 AGGCCCC [GA]G^G[ACGT]CC[CT] 406 AGGACCC EagI 319 CGGCCG C^GGCCG 773 CGGCCG EclXI 319 CGGCCG C^GGCCG 773 CGGCCG Eco52I 319 CGGCCG C^GGCCG 773 CGGCCG EcoO109I 74 AGGCCCC [GA]G^G[ACGT]CC[CT] 406 AGGACCC HphI 627 GGTGA GGTGA(8/7) 518 TCACC KasI 222 GGCGCC G^GCGCC 837 GGCGCC NaeI 51 GCCGGC GCC^GGC 444 GCCGGC NarI 222 GGCGCC GG^CGCC 837 GGCGCC NgoMIV 51 GCCGGC G^CCGGC 444 GCCGGC NotI 318 GCGGCCGC GC^GGCCGC 772 GCGGCCGC PvuII 529 CAGCTG CAG^CTG 764 CAGCTG RsaI 567 GTAC GT^AC 879 GTAC SfaNI 367 GATGC GCATC(5/9) 417 GATGC SfcI 767 CTGTAG C^T[GA][CT]AG 870 CTACAG SfoI 222 GGCGCC GGC^GCC 837 GGCGCC TspRI 266 CACTG CA[GC]TG[ACGT][ACGT]^ 615 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AccII AciI AcyI AluI AlwNI BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BsiYI BslI BsmFI Bsp1286I BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DsaI EaeI EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI KspI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI SacII Sau96I ScrFI StyI TseI