##################################
# NO CUT ENZYMES (  198 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaLI       AscI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaI        BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      ClaI        CpoI        DdeI        DpnII       
DraI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HindIII     
HinfI       HpaI        KasI        KpnI        KspI        
MaeII       MaeIII      MboI        MfeI        MflI        
MluI        MroI        MslI        MspA1I      MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
Sau3AI      SbfI        ScaI        SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        TfiI        Tsp45I      Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AciI          308  CCGC                 CCGC(-3/-1)
AcsI          154  GAATTT               [GA]^AATT[CT]
ApaI           23  GGGCCC               GGGCC^C
ApoI          154  GAATTT               [GA]^AATT[CT]
AseI          181  ATTAAT               AT^TAAT
AsnI          181  ATTAAT               AT^TAAT
BalI           65  TGGCCA               TGG^CCA
BanII          23  GGGCCC               G[GA]GC[CT]^C
BfaI           94  CTAG                 C^TAG
BmyI           23  GGGCCC               G[AGT]GC[ACT]^C
BsaBI         193  GATAAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI          68  CCAAGG               C^C[ACGT][ACGT]GG
BsaWI          86  ACCGGA               [AT]^CCGG[AT]
BseRI         209  CTCCTC               GAGGAG(10/8)
BsmAI          38  GAGAC                GTCTC(1/5)
Bsp1286I       23  GGGCCC               G[AGT]GC[ACT]^C
BstF5I         45  GGATG                GGATG(2/0)
DraII          23  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EaeI           65  TGGCCA               [CT]^GGCC[GA]
EcoO109I       23  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I        68  CCAAGG               C^C[AT][AT]GG
FokI           45  GGATG                GGATG(9/13)
HapII          87  CCGG                 C^CGG
HincII         82  GTCAAC               GT[CT]^[GA]AC
HindII         82  GTCAAC               GT[CT]^[GA]AC
HpaII          87  CCGG                 C^CGG
HphI           51  GGTGA                GGTGA(8/7)
MaeI           94  CTAG                 C^TAG
MamI          193  GATAAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI          65  TGGCCA               TGG^CCA
MscI           65  TGGCCA               TGG^CCA
MseI          182  TTAA                 T^TAA
MspI           87  CCGG                 C^CGG
NlaIV          23  GGGCCC               GG[ACGT]^[ACGT]CC
PshBI         181  ATTAAT               AT^TAAT
SapI          343  GCTCTTC              GCTCTTC(1/4)
StyI           68  CCAAGG               C^C[AT][AT]GG
Tru9I         182  TTAA                 T^TAA
TspRI         321  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AluI          140  AGCT                 AG^CT
              342  AGCT                
BpmI          188  CTCCAG               CTGGAG(16/14)
              349  CTCCAG              
BssKI         150  CCTGG                ^CC[ACGT]GG
              330  CCAGG               
BstNI         150  CCTGG                CC^[AT]GG
              330  CCAGG               
EcoRII        150  CCTGG                ^CC[AT]GG
              330  CCAGG               
MvaI          150  CCTGG                CC^[AT]GG
              330  CCAGG               
MwoI          310  GCCTTTGAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              334  GCAATCAAAGC         
NlaIII         18  CATG                 CATG^
              238  CATG                
ScrFI         150  CCTGG                CC^[ACGT]GG
              330  CCAGG               
Tsp509I       155  AATT                 ^AATT
              254  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

BbvI        Cfr13I      EarI        Fnu4HI      HaeIII      
ItaI        Ksp632I     MboII       MnlI        Sau96I      
TseI