Restriction map for
pF1KE0531
##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AccIII AclI AcsI AflII AgeI
AhdI Alw44I ApaLI ApoI AscI
AseI AsnI AspEI AspI AviII
AvrII BbeI BbrPI BbvCI BcgI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BseAI BsgI BsiEI BsiWI
BsmBI BspDI BspEI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco52I
EcoNI EcoRI EcoRV EcoT22I FspI
HincII HindII HindIII HpaI KasI
KspI MfeI MluI MroI MunI
NarI NdeI NotI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AatII 1108 GACGTC GACGT^C
AcyI 1108 GACGTC G[GA]^CG[CT]C
AflIII 30 ACATGT A^C[GA][CT]GT
AlwNI 40 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 926 CCTTAGG CC^T[ACGT]AGG
Aor51HI 287 AGCGCT AGC^GCT
Asp700I 687 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI 1200 GTATCC GTATCC(6/5)
BfaI 1004 CTAG C^TAG
BglI 802 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1216 CTGGAG CTGGAG(16/14)
BsaBI 906 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1108 GACGTC G[GA]^CG[CT]C
BsaI 778 GGTCTC GGTCTC(1/5)
BsaWI 179 ACCGGA [AT]^CCGG[AT]
BsmAI 779 GTCTC GTCTC(1/5)
Bsp1407I 977 TGTACA T^GTACA
BspHI 902 TCATGA T^CATGA
BspLU11I 30 ACATGT A^CATGT
BsrBI 490 GAGCGG CCGCTC(-3/-3)
BsrFI 727 GCCGGC [GA]^CCGG[CT]
BsrGI 977 TGTACA T^GTACA
BstEII 385 GGTTACC G^GT[ACGT]ACC
BstPI 385 GGTTACC G^GT[ACGT]ACC
Bsu36I 926 CCTTAGG CC^T[ACGT]AGG
Cfr10I 727 GCCGGC [GA]^CCGG[CT]
DraII 131 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 406 GACAAGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 80 CCATGG C^C[GA][CT]GG
EarI 160 CTCTTC CTCTTC(1/4)
Eco47III 287 AGCGCT AGC^GCT
Eco81I 926 CCTTAGG CC^T[ACGT]AGG
EcoO109I 131 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 385 GGTTACC G^GT[ACGT]ACC
FseI 726 GGCCGGCC GGCCGG^CC
HgaI 941 GACGC GACGC(5/10)
Hin1I 1108 GACGTC G[GA]^CG[CT]C
Ksp632I 160 CTCTTC CTCTTC(1/4)
MaeI 1004 CTAG C^TAG
MamI 906 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 119 TTAA T^TAA
NaeI 727 GCCGGC GCC^GGC
NcoI 80 CCATGG C^CATGG
NgoMIV 727 GCCGGC G^CCGGC
NheI 1003 GCTAGC G^CTAGC
NruI 252 TCGCGA TCG^CGA
PleI 658 GACTC GAGTC(4/5)
PpuMI 131 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 902 TCATGA T^CATGA
SapI 159 GCTCTTC GCTCTTC(1/4)
SfiI 801 GGCCCAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 797 CCCGGG CCC^GGG
SmlI 457 CTCAAG C^T[CT][GA]AG
SspBI 977 TGTACA T^GTACA
TaqI 971 TCGA T^CGA
Tru9I 119 TTAA T^TAA
Tsp45I 1121 GTGAC ^GT[GC]AC
Tsp509I 688 AATT ^AATT
TthHB8I 971 TCGA T^CGA
XmaCI 797 CCCGGG C^CCGGG
XmaI 797 CCCGGG C^CCGGG
XmnI 687 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 503 GGTACC G^GTACC
1193 GGTACC
AccI 448 GTAGAC GT^[AC][GT]AC
1111 GTCTAC
AccII 253 CGCG CG^CG
357 CGCG
ApaI 494 GGGCCC GGGCC^C
800 GGGCCC
Asp718I 503 GGTACC G^GTACC
1193 GGTACC
AvaI 484 CCCGAG C^[CT]CG[GA]G
797 CCCGGG
BalI 565 TGGCCA TGG^CCA
1065 TGGCCA
BamHI 279 GGATCC G^GATCC
921 GGATCC
BbsI 9 GAAGAC GAAGAC(2/6)
1015 GTCTTC
BclI 905 TGATCA T^GATCA
1163 TGATCA
BpuAI 9 GAAGAC GAAGAC(2/6)
1015 GTCTTC
BseRI 1056 CTCCTC GAGGAG(10/8)
487 GAGGAG
BsmI 528 GAATGC GAATGC(1/-1)
302 GCATTC
BsoBI 484 CCCGAG C^[CT]CG[GA]G
797 CCCGGG
BspMI 376 ACCTGC ACCTGC(4/8)
854 ACCTGC
BstUI 253 CGCG CG^CG
357 CGCG
BstYI 279 GGATCC [GA]^GATC[CT]
921 GGATCC
CfoI 288 GCGC GCG^C
1007 GCGC
FauI 201 GCGGG CCCGC(4/6)
492 GCGGG
FbaI 905 TGATCA T^GATCA
1163 TGATCA
HaeII 287 AGCGCT [GA]GCGC^[CT]
1006 AGCGCC
HhaI 288 GCGC GCG^C
1007 GCGC
HinP1I 288 GCGC G^CGC
1007 GCGC
KpnI 503 GGTACC GGTAC^C
1193 GGTACC
MaeII 323 ACGT A^CGT
1109 ACGT
MflI 279 GGATCC [GA]^GATC[CT]
921 GGATCC
MluNI 565 TGGCCA TGG^CCA
1065 TGGCCA
MscI 565 TGGCCA TGG^CCA
1065 TGGCCA
MvnI 253 CGCG CG^CG
357 CGCG
PflMI 80 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
873 CCAGAACATGG
TfiI 894 GAATC G^A[AT]TC
948 GATTC
Van91I 80 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
873 CCAGAACATGG
XhoII 279 GGATCC [GA]^GATC[CT]
921 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanI BanII BbvI BcnI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
TspRI XcmI