Restriction map for
pF1KE0343
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
Alw44I ApaLI ApoI AscI AseI
AsnI AspI AviII AvrII BamHI
BbeI BbvCI BcgI BciVI BfrI
BlnI BsaBI BsiEI BsiWI BsmBI
BsmI BspDI BspHI BspLU11I BspMI
BsrDI BssSI Bst1107I BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
EagI EclXI Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KasI
KspI MaeIII MamI MfeI MluI
MseI MunI NarI NdeI NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfoI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Tru9I Tsp45I Tsp509I
Tth111I XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 1094 GGTACC G^GTACC
AccIII 1211 TCCGGA T^CCGGA
AcyI 411 GACGCC G[GA]^CG[CT]C
AhdI 1732 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1775 AGCGCT AGC^GCT
ApaI 905 GGGCCC GGGCC^C
Asp718I 1094 GGTACC G^GTACC
AspEI 1732 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1426 GAGCTC G[AT]GC[AT]^C
BanI 1094 GGTACC G^G[CT][GA]CC
BbsI 1288 GAAGAC GAAGAC(2/6)
BclI 405 TGATCA T^GATCA
BfaI 1315 CTAG C^TAG
BglII 116 AGATCT A^GATCT
BpuAI 1288 GAAGAC GAAGAC(2/6)
BsaHI 411 GACGCC G[GA]^CG[CT]C
BsaI 1572 GGTCTC GGTCTC(1/5)
BsaWI 1211 TCCGGA [AT]^CCGG[AT]
BseAI 1211 TCCGGA T^CCGGA
BsiHKAI 1426 GAGCTC G[AT]GC[AT]^C
Bsp1407I 494 TGTACA T^GTACA
BspEI 1211 TCCGGA T^CCGGA
BsrFI 1632 GCCGGC [GA]^CCGG[CT]
BsrGI 494 TGTACA T^GTACA
BssHII 16 GCGCGC G^CGCGC
BstBI 1412 TTCGAA TT^CGAA
Cfr10I 1632 GCCGGC [GA]^CCGG[CT]
Eam1105I 1732 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1775 AGCGCT AGC^GCT
FbaI 405 TGATCA T^GATCA
Hin1I 411 GACGCC G[GA]^CG[CT]C
KpnI 1094 GGTACC GGTAC^C
MaeI 1315 CTAG C^TAG
MroI 1211 TCCGGA T^CCGGA
MslI 1552 CACACCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1632 GCCGGC GCC^GGC
NgoMIV 1632 GCCGGC G^CCGGC
NspV 1412 TTCGAA TT^CGAA
PflMI 56 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 1426 GAGCTC GAGCT^C
SapI 1428 GCTCTTC GCTCTTC(1/4)
SfuI 1412 TTCGAA TT^CGAA
SmaI 1711 CCCGGG CCC^GGG
SmlI 397 CTCAAG C^T[CT][GA]AG
SspBI 494 TGTACA T^GTACA
TfiI 1279 GATTC G^A[AT]TC
Van91I 56 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1314 TCTAGA T^CTAGA
XmaCI 1711 CCCGGG C^CCGGG
XmaI 1711 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 196 GTCTAC GT^[AC][GT]AC
232 GTCTAC
AccII 17 CGCG CG^CG
46 CGCG
AflIII 545 ACACGT A^C[GA][CT]GT
1185 ACACGT
AocI 802 CCTGAGG CC^T[ACGT]AGG
1321 CCTGAGG
Asp700I 345 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
528 GAAGGATTTC
AvaI 1339 CCCGAG C^[CT]CG[GA]G
1711 CCCGGG
BbrPI 546 CACGTG CAC^GTG
1186 CACGTG
BglI 451 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
814 GCCCCCAAGGC
BlpI 1237 GCTGAGC GC^T[ACGT]AGC
1470 GCTGAGC
Bpu1102I 1237 GCTGAGC GC^T[ACGT]AGC
1470 GCTGAGC
BsaAI 546 CACGTG [CT]AC^GT[GA]
1186 CACGTG
BsoBI 1339 CCCGAG C^[CT]CG[GA]G
1711 CCCGGG
BsrBI 824 CCGCTC CCGCTC(-3/-3)
895 GAGCGG
BstUI 17 CGCG CG^CG
46 CGCG
BstYI 116 AGATCT [GA]^GATC[CT]
393 GGATCT
Bsu36I 802 CCTGAGG CC^T[ACGT]AGG
1321 CCTGAGG
CelII 1237 GCTGAGC GC^T[ACGT]AGC
1470 GCTGAGC
DrdI 1162 GACATGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1564 GACCAGCTGGTC
EarI 282 CTCTTC CTCTTC(1/4)
1429 CTCTTC
Eco81I 802 CCTGAGG CC^T[ACGT]AGG
1321 CCTGAGG
HaeII 1063 GGCGCT [GA]GCGC^[CT]
1775 AGCGCT
HphI 508 GGTGA GGTGA(8/7)
53 TCACC
Ksp632I 282 CTCTTC CTCTTC(1/4)
1429 CTCTTC
MflI 116 AGATCT [GA]^GATC[CT]
393 GGATCT
MvnI 17 CGCG CG^CG
46 CGCG
PmaCI 546 CACGTG CAC^GTG
1186 CACGTG
PmlI 546 CACGTG CAC^GTG
1186 CACGTG
SfiI 450 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
813 GGCCCCCAAGGCC
StuI 806 AGGCCT AGG^CCT
1003 AGGCCT
TaqI 1413 TCGA T^CGA
1640 TCGA
TthHB8I 1413 TCGA T^CGA
1640 TCGA
XcmI 446 CCATGGCCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
818 CCAAGGCCGCTCTGG
XhoII 116 AGATCT [GA]^GATC[CT]
393 GGATCT
XmnI 345 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
528 GAAGGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BalI BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI MaeII MboI
MboII MluNI MnlI MscI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI TspRI