################################## # NO CUT ENZYMES ( 191 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeIII HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MboI MfeI MflI MluI MluNI MscI MslI MspA1I MunI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccIII 254 TCCGGA T^CCGGA AcyI 11 GGCGTC G[GA]^CG[CT]C BbvCI 124 GCTGAGG CCTCAGC(-5/-2) BsaHI 11 GGCGTC G[GA]^CG[CT]C BsaI 103 GGTCTC GGTCTC(1/5) BsaWI 254 TCCGGA [AT]^CCGG[AT] BseAI 254 TCCGGA T^CCGGA BsmAI 104 GTCTC GTCTC(1/5) BspEI 254 TCCGGA T^CCGGA BspMI 48 GCAGGT ACCTGC(4/8) BssKI 308 CCTGG ^CC[ACGT]GG BstF5I 211 GGATG GGATG(2/0) BstNI 308 CCTGG CC^[AT]GG DdeI 125 CTGAG C^T[ACGT]AG DraI 274 TTTAAA TTT^AAA DraII 50 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoO109I 50 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoRII 308 CCTGG ^CC[AT]GG EcoT14I 99 CCTTGG C^C[AT][AT]GG FokI 211 GGATG GGATG(9/13) HaeII 121 GGCGCT [GA]GCGC^[CT] HapII 255 CCGG C^CGG HgaI 12 GCGTC GACGC(5/10) Hin1I 11 GGCGTC G[GA]^CG[CT]C HpaII 255 CCGG C^CGG MboII 298 GAAGA GAAGA(8/7) MroI 254 TCCGGA T^CCGGA MseI 275 TTAA T^TAA MspI 255 CCGG C^CGG MvaI 308 CCTGG CC^[AT]GG PpuMI 50 AGGTCCC [GA]G^G[AT]CC[CT] PstI 131 CTGCAG CTGCA^G ScrFI 308 CCTGG CC^[ACGT]GG StyI 99 CCTTGG C^C[AT][AT]GG Tru9I 275 TTAA T^TAA Tsp45I 14 GTCAC ^GT[GC]AC Tsp509I 284 AATT ^AATT ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccII 37 CGCG CG^CG 77 CGCG AluI 65 AGCT AG^CT 135 AGCT AvaI 54 CCCGAG C^[CT]CG[GA]G 106 CTCGGG AvaII 51 GGTCC G^G[AT]CC 168 GGACC BsaJI 55 CCGAGG C^C[ACGT][ACGT]GG 99 CCTTGG BsoBI 54 CCCGAG C^[CT]CG[GA]G 106 CTCGGG BstUI 37 CGCG CG^CG 77 CGCG Cac8I 8 GCTGGC GC[ACGT]^[ACGT]GC 32 GCGGGC Cfr13I 51 GGTCC G^G[ACGT]CC 168 GGACC HphI 144 GGTGA GGTGA(8/7) 15 TCACC MaeIII 14 GTCAC ^GT[ACGT]AC 304 GTTAC MvnI 37 CGCG CG^CG 77 CGCG NlaIII 141 CATG CATG^ 181 CATG NlaIV 51 GGTCCC GG[ACGT]^[ACGT]CC 167 GGGACC Sau96I 51 GGTCC G^G[ACGT]CC 168 GGACC SfcI 116 CTGTAG C^T[GA][CT]AG 131 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AciI BbvI BsmFI BsrI CfoI FauI Fnu4HI HhaI HinP1I ItaI MnlI MwoI TseI TspRI