################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrI BglI BlnI BpmI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SpeI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AlwI 292 GGATC GGATC(4/5) ApaI 943 GGGCCC GGGCC^C AseI 1488 ATTAAT AT^TAAT AsnI 1488 ATTAAT AT^TAAT AspHI 1239 GAGCAC G[AT]GC[AT]^C AvaII 1174 GGTCC G^G[AT]CC BbrPI 1246 CACGTG CAC^GTG BglII 84 AGATCT A^GATCT BlpI 871 GCTGAGC GC^T[ACGT]AGC Bpu1102I 871 GCTGAGC GC^T[ACGT]AGC BseRI 565 GAGGAG GAGGAG(10/8) BsiHKAI 1239 GAGCAC G[AT]GC[AT]^C BspMI 903 ACCTGC ACCTGC(4/8) CelII 871 GCTGAGC GC^T[ACGT]AGC CfoI 370 GCGC GCG^C DraIII 1075 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 632 CCATGG C^C[GA][CT]GG EcoRI 1012 GAATTC G^AATTC EcoT14I 632 CCATGG C^C[AT][AT]GG HhaI 370 GCGC GCG^C HinP1I 370 GCGC G^CGC HindIII 977 AAGCTT A^AGCTT NcoI 632 CCATGG C^CATGG PmaCI 1246 CACGTG CAC^GTG PmlI 1246 CACGTG CAC^GTG PshBI 1488 ATTAAT AT^TAAT PstI 1747 CTGCAG CTGCA^G SapI 1135 GAAGAGC GCTCTTC(1/4) SnaBI 1502 TACGTA TAC^GTA SspI 601 AATATT AAT^ATT StyI 632 CCATGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BbsI 781 GAAGAC GAAGAC(2/6) 463 GTCTTC BcnI 25 CCGGG CC^[GC]GG 442 CCCGG BpuAI 781 GAAGAC GAAGAC(2/6) 463 GTCTTC BsaBI 79 GATATAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1582 GATTTAGATC BsrDI 468 CATTGC GCAATG(2/0) 1766 GCAATG BstF5I 273 CATCC GGATG(2/0) 768 CATCC BstYI 84 AGATCT [GA]^GATC[CT] 292 GGATCT EarI 232 GAAGAG CTCTTC(1/4) 1135 GAAGAG FokI 273 CATCC GGATG(9/13) 768 CATCC HaeIII 944 GGCC GG^CC 1114 GGCC Ksp632I 232 GAAGAG CTCTTC(1/4) 1135 GAAGAG MamI 79 GATATAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1582 GATTTAGATC MflI 84 AGATCT [GA]^GATC[CT] 292 GGATCT MslI 1242 CACTCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1246 CACGTGCATG NciI 25 CCGGG CC^[GC]GG 442 CCCGG NlaIV 636 GGTTCC GG[ACGT]^[ACGT]CC 943 GGGCCC PleI 29 GACTC GAGTC(4/5) 1027 GACTC PshAI 29 GACTCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 112 GACTTCAGTC XhoII 84 AGATCT [GA]^GATC[CT] 292 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BanII BbvI BfaI BmyI BsaAI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI HapII HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NspI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SphI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I