Restriction map for
pF1KA1565
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# NO CUT ENZYMES ( 85 enzymes) #
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AatII AccI AccIII AflII AgeI
AhdI Aor51HI AscI AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BfrI BglI BlnI
BsaAI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BsrGI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV FseI
FspI HincII HindII HpaI KasI
KspI MluI MroI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PvuI RsrII SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI Tth111I XcmI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 3544 GGTACC G^GTACC
AccII 398 CGCG CG^CG
AclI 2792 AACGTT AA^CGTT
AcyI 3820 GACGCC G[GA]^CG[CT]C
AflIII 113 ACATGT A^C[GA][CT]GT
Alw44I 1685 GTGCAC G^TGCAC
ApaI 3311 GGGCCC GGGCC^C
ApaLI 1685 GTGCAC G^TGCAC
AseI 210 ATTAAT AT^TAAT
AsnI 210 ATTAAT AT^TAAT
Asp718I 3544 GGTACC G^GTACC
BalI 1085 TGGCCA TGG^CCA
BbvCI 2154 GCTGAGG CCTCAGC(-5/-2)
BglII 1164 AGATCT A^GATCT
BlpI 280 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 280 GCTCAGC GC^T[ACGT]AGC
BsaBI 2399 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3820 GACGCC G[GA]^CG[CT]C
BsaI 621 GGTCTC GGTCTC(1/5)
BsaWI 608 ACCGGA [AT]^CCGG[AT]
BspHI 2070 TCATGA T^CATGA
BspLU11I 113 ACATGT A^CATGT
BsrDI 853 GCAATG GCAATG(2/0)
BsrFI 418 GCCGGT [GA]^CCGG[CT]
BstAPI 1326 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 398 CGCG CG^CG
CelII 280 GCTCAGC GC^T[ACGT]AGC
Cfr10I 418 GCCGGT [GA]^CCGG[CT]
DraIII 515 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 3645 GAATTC G^AATTC
EcoT22I 911 ATGCAT ATGCA^T
FauI 2217 GCGGG CCCGC(4/6)
HaeII 2242 AGCGCC [GA]GCGC^[CT]
Hin1I 3820 GACGCC G[GA]^CG[CT]C
KpnI 3544 GGTACC GGTAC^C
MamI 2399 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1085 TGGCCA TGG^CCA
MscI 1085 TGGCCA TGG^CCA
MvnI 398 CGCG CG^CG
NcoI 3440 CCATGG C^CATGG
NdeI 1863 CATATG CA^TATG
NsiI 911 ATGCAT ATGCA^T
NspI 113 ACATGT [GA]CATG^[CT]
PaeR7I 670 CTCGAG C^TCGAG
PshAI 317 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 210 ATTAAT AT^TAAT
Psp1406I 2792 AACGTT AA^CGTT
RcaI 2070 TCATGA T^CATGA
SbfI 2142 CCTGCAGG CCTGCA^GG
SmaI 3476 CCCGGG CCC^GGG
Sse8387I 2142 CCTGCAGG CCTGCA^GG
XbaI 1461 TCTAGA T^CTAGA
XhoI 670 CTCGAG C^TCGAG
XmaCI 3476 CCCGGG C^CCGGG
XmaI 3476 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AocI 1254 CCTCAGG CC^T[ACGT]AGG
3805 CCTGAGG
BanI 797 GGCACC G^G[CT][GA]CC
3544 GGTACC
BclI 1215 TGATCA T^GATCA
3594 TGATCA
BsmAI 1593 GAGAC GTCTC(1/5)
622 GTCTC
BspMI 2141 ACCTGC ACCTGC(4/8)
3194 ACCTGC
Bsu36I 1254 CCTCAGG CC^T[ACGT]AGG
3805 CCTGAGG
EaeI 1085 TGGCCA [CT]^GGCC[GA]
1226 CGGCCA
Eco81I 1254 CCTCAGG CC^T[ACGT]AGG
3805 CCTGAGG
EcoNI 81 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1332 CCTGCAGCAGG
EcoT14I 983 CCTTGG C^C[AT][AT]GG
3440 CCATGG
FbaI 1215 TGATCA T^GATCA
3594 TGATCA
HgaI 444 GACGC GACGC(5/10)
3820 GACGC
MfeI 2620 CAATTG C^AATTG
3493 CAATTG
MslI 2814 CACTTTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3112 CATCAGAGTG
MunI 2620 CAATTG C^AATTG
3493 CAATTG
PflMI 2616 CCAACAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3435 CCAGACCATGG
PpuMI 76 GGGTCCC [GA]G^G[AT]CC[CT]
322 AGGTCCT
StyI 983 CCTTGG C^C[AT][AT]GG
3440 CCATGG
Van91I 2616 CCAACAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3435 CCAGACCATGG
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# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmFI BsmI BsoBI Bsp1286I BsrBI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EarI EcoO109I EcoO65I EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI