Restriction map for
pF1KE1067
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII Acc65I AccI AclI AflII
AflIII AgeI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AviII AvrII BbeI BbrPI
BbsI BcgI BclI BcnI BfrI
BlnI BpuAI BsaBI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspLU11I BsrDI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Cfr10I CpoI
DraIII EagI EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MslI MunI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacII SalI SbfI ScaI SexAI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccIII 1144 TCCGGA T^CCGGA
AcsI 694 GAATTC [GA]^AATT[CT]
AcyI 70 GACGCC G[GA]^CG[CT]C
AhdI 1429 GACTCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1480 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1253 CCTTAGG CC^T[ACGT]AGG
ApoI 694 GAATTC [GA]^AATT[CT]
Asp700I 378 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1429 GACTCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 543 TGGCCA TGG^CCA
BamHI 50 GGATCC G^GATCC
BanI 406 GGTGCC G^G[CT][GA]CC
BbvCI 967 GCTGAGG CCTCAGC(-5/-2)
BciVI 170 GGATAC GTATCC(6/5)
BglII 380 AGATCT A^GATCT
BlpI 510 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 510 GCTGAGC GC^T[ACGT]AGC
BsaAI 106 TACGTA [CT]AC^GT[GA]
BsaHI 70 GACGCC G[GA]^CG[CT]C
BsaI 587 GAGACC GGTCTC(1/5)
BsaWI 1144 TCCGGA [AT]^CCGG[AT]
BseAI 1144 TCCGGA T^CCGGA
BspDI 652 ATCGAT AT^CGAT
BspEI 1144 TCCGGA T^CCGGA
BspHI 360 TCATGA T^CATGA
BsrBI 1140 CCGCTC CCGCTC(-3/-3)
BssSI 1431 CTCGTG CACGAG(-5/-1)
BstAPI 39 GCACGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1253 CCTTAGG CC^T[ACGT]AGG
CelII 510 GCTGAGC GC^T[ACGT]AGC
ClaI 652 ATCGAT AT^CGAT
DrdI 852 GACACCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1429 GACTCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 450 GAAGAG CTCTTC(1/4)
Eco81I 1253 CCTTAGG CC^T[ACGT]AGG
EcoRI 694 GAATTC G^AATTC
HaeII 1338 GGCGCT [GA]GCGC^[CT]
HgaI 70 GACGC GACGC(5/10)
Hin1I 70 GACGCC G[GA]^CG[CT]C
HindIII 268 AAGCTT A^AGCTT
Ksp632I 450 GAAGAG CTCTTC(1/4)
MluNI 543 TGGCCA TGG^CCA
MroI 1144 TCCGGA T^CCGGA
MscI 543 TGGCCA TGG^CCA
NcoI 1103 CCATGG C^CATGG
NspI 1031 ACATGC [GA]CATG^[CT]
PaeR7I 398 CTCGAG C^TCGAG
RcaI 360 TCATGA T^CATGA
SacI 1357 GAGCTC GAGCT^C
SapI 450 GAAGAGC GCTCTTC(1/4)
SfiI 621 GGCCTCCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 398 CTCGAG C^T[CT][GA]AG
SnaBI 106 TACGTA TAC^GTA
Tsp509I 695 AATT ^AATT
XbaI 717 TCTAGA T^CTAGA
XcmI 134 CCAACAGCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 398 CTCGAG C^TCGAG
XmnI 378 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
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AspHI 1159 GTGCTC G[AT]GC[AT]^C
1357 GAGCTC
AvaI 398 CTCGAG C^[CT]CG[GA]G
1306 CCCGAG
BfaI 5 CTAG C^TAG
718 CTAG
BglI 613 GCCGAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
622 GCCTCCACGGC
BsiHKAI 1159 GTGCTC G[AT]GC[AT]^C
1357 GAGCTC
BsmFI 667 GTCCC GGGAC(10/14)
1437 GTCCC
BsmI 534 GAATGC GAATGC(1/-1)
1197 GCATTC
BsoBI 398 CTCGAG C^[CT]CG[GA]G
1306 CCCGAG
BstXI 546 CCAGGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
600 CCAGCTCACTGG
DraI 184 TTTAAA TTT^AAA
436 TTTAAA
DsaI 626 CCACGG C^C[GA][CT]GG
1103 CCATGG
EaeI 543 TGGCCA [CT]^GGCC[GA]
629 CGGCCA
EcoNI 794 CCTCACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1253 CCTTAGGTAGG
HapII 168 CCGG C^CGG
1145 CCGG
HpaII 168 CCGG C^CGG
1145 CCGG
MaeI 5 CTAG C^TAG
718 CTAG
MseI 185 TTAA T^TAA
437 TTAA
MspA1I 856 CCGCTG C[AC]G^C[GT]G
1207 CCGCTG
MspI 168 CCGG C^CGG
1145 CCGG
StuI 488 AGGCCT AGG^CCT
837 AGGCCT
Tru9I 185 TTAA T^TAA
437 TTAA
Tsp45I 709 GTGAC ^GT[GC]AC
1166 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BanII BbvI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MvaI MvnI MwoI
NdeII NlaIII NlaIV PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
TseI TspRI TthHB8I XhoII